Effects of Oregano Methanolic Extract on the Chemical, Microbial, and Sensory Properties of Yogurt

Document Type : Research Paper

Authors

Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

Abstract

Introduction: The demand for antimicrobial compound alternatives to replace synthetic additives is on the rise. In food commodities, use of synthetic antimicrobials based on herbal extracts is attracted attention. The present study aimed to assess the chemical, microbiological, and sensory properties of the yogurt samples treated with oregano extract (0%, 0.75%, 1%, and 1.5%) during storage for 30 days at refrigerated temperature. Methods: The in-vitro antibacterial effect of oregano extract was evaluated using agar disk-diffusion assay. Natural yogurt was prepared using a combination mixture of whole milk and 4% skimmed milk powder. Various concentrations of the oregano extract (0%, 0.75%, 1%, and 1.5%) were added to the samples. During 30 days of refrigerated storage, various parameters were evaluated, including titratable acidity, pH, mold and yeast counts, total coliforms, and sensory attributes (overall liking, appearance, and aroma). Results: The descending order of the in-vitro antibacterial effect of the oregano extract was as follows: Listeria monocytogenes>Staphylococcus aureus>Bacillus subtilis>Bacillus cereus>Salmonella typhimurium>Escherichia coli O157:H7. Differences were observed in the counts of mold and yeast in the samples over time. Accordingly, bioactive yogurt had lower mold and yeast counts (1-1.5 log CFU/g) after 30 days of storage compared to the control (P<0.05). In addition, significant differences were observed in the hedonic scores of aroma and appearance between the samples containing the oregano extract compared to the control group (P<0.05). Conclusion: According to the results, oregano extract could be used as a natural compound to improve the shelf life of yogurt at refrigerated temperatures for 30 days. Furthermore, oregano extract is considered to be an effective inhibitory compound against L. monocytogenes, S. aureus, B. subtilis, B. cereus, S. typhimurium, and E. coli O157:H7.

Keywords


  1. Shahbazi Y. Ziziphora clinopodioides essential oil and nisin as potential antimicrobial agents againstEscherichia coliO157:H7 in doogh (Iranian yoghurt drink). J Pathog. 2015; 2015: 176024.
  2. Shahbazi Y, Shavisi N. Fate of Listeria monocytogenesduring ripening of Iranian traditional koozeh cheese made from raw ewe's milk. J Food Qual Hazards Control. 2018; 5(3): 109-15.
  3. Fazilah NF, Ariff AB, Khayat ME, Rios-Solis L, Halim M. Influence of probiotics, prebiotics, synbiotics and bioactive phytochemicals on the formulation of functional yogurt. J Funct Foods. 2018; 48: 387-99.
  4. Zhao L, Feng R, Ren F, Mao X. Addition of buttermilk improves the flavor and volatile compound profiles of low-fat yogurt. LWT. 2018; 98: 9-17.
  5. Mudgil P, Jumah B, Ahmad M, Hamed F, Maqsood S. Rheological, micro-structural and sensorial properties of camel milk yogurt as influenced by gelatin. LWT. 2018; 98: 646-53.
  6. Aryana KJ, Olson DW. A 100-year review: Yogurt and other cultured dairy products. J Dairy Sci. 2017; 100(12): 9987-10013.
  7. Parvez S, Malik KA, Ah Kang S, Kim HY. Probiotics and their fermented food products are beneficial for health. J Appl Microbiol. 2006; 100(6): 1171-85.
  8. Sharma R, Bhaskar B, Sanodiya BS, Thakur GS, Jaiswal P, Yadav N, et al. Probiotic efficacy and potential of Streptococcus thermophiles modulating human health: A synoptic review. IOSR J Pharm Biol Sci. 2014; 9(3): 52-8.
  9. Kaminarides S, Stamou P, Massouras T. Comparison of the characteristics of set type yoghurt made from ovine milk of different fat content. Int J Food Sci Technol. 2007; 42(9): 1019-28.
  10. Shahbazi Y. Effects of Ziziphora clinopodioides essential oil and nisin on the microbiological properties of milk. Pharm Sci. 2016; 22(4): 272-8.
  11. Shahbazi Y. Application of carboxymethyl cellulose and chitosan coatings containing Mentha spicata essential oil in fresh strawberries. Int J Biol Macromol. 2018; 112: 264-72.
  12. Van Haute S, Raes K, Van Der Meeren P, Sampers I. The effect of cinnamon, oregano and thyme essential oils in marinade on the microbial shelf life of fish and meat products. Food Control. 2016; 68: 30-9.
  13. Shekarforoush SS, Basiri S, Ebrahimnejad H, Hosseinzadeh S. Effect of chitosan on spoilage bacteria, Escherichiacoli and Listeria monocytogenes in cured chicken meat. Int J Biol Macromol. 2015; 76: 303-9.
  14. Dutra TV, Castro JC, Menezes JL, Ramos TR, do Prado IN, Junior MM, et al. Bioactivity of oregano (Origanum vulgare) essential oil against Alicyclobacillus spp. Ind Crops Prod. 2019; 129: 345-9.
  15. Camo J, Lorés A, Djenane D, Beltrán JA, Roncalés P. Display life of beef packaged with an antioxidant active film as a function of the concentration of oregano extract. Meat Sci. 2011; 88(1): 174-8.
  16. Shahbazi Y, Shavisi N. Effects of oregano extract on the inhibition of selected pathogens in raw beef meat. J Fasting Health. 2017; 6(1): 15-22.
  17. Shahbazi Y, Shavisi N, Modarresi M, Karami N. Chemical composition, antibacterial and antioxidant activities of essential oils from the aerial parts of Ferulago angulata(Schlecht.) boiss and Ferulago bernardiiTomk. & M. Pimen from different prts of Iran. Journal of Essential Oil Bearing Plants. 2016; 19(7): 1627-38.
  18. Pang Z, Xu R, Luo T, Che X, Bansal N, Liu X. Physiochemical properties of modified starch under yogurt manufacturing conditions and its relation to the properties of yogurt. J Food Eng. 2019; 245: 11-7.
  19. AOAC. Official methods of analysis, 16th ed. Association of official analytical chemists, arlington, va, USA.  1995.
  20. Jay JM, Loessner MJ, Golden DA. Modern food microbiology, 7th ed.,. New York, NY: Springer Science Business Media, Inc,; 2005.
  21. Gyawali R, Ibrahim SA. Natural products as antimicrobial agents. Food Control. 2014; 46: 412-29.
  22. Burt S. Essential oils: Their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol. 2004; 94(3): 223-53.
  23. Klančnik A, Piskernik S, Jeršek B, Možina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods. 2010; 81(2): 121-6.
  24. Lv F, Liang H, Yuan Q, Li C. In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms. Food Res Int 2011; 44(9): 3057-64.
  25. Gilles M, Zhao J, An M, Agboola S. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chem. 2010; 119(2): 731-7.
  26. Teixeira B, Marques A, Ramos C, Batista I, Serrano C, Matos O, et al. European pennyroyal (Mentha pulegium) from portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Ind Crops Prod. 2012; 36(1): 81-7.
  27. El-Ziney M, Abdel-Salam AM. Chemical and anti-bacterial characterization of aqueous extracts of oregano, marjoram, sage and licorice and their application in milk and labneh. J Food Agric Environ. 2007; 6(1): 39-42.
  28. Tabasco R, Sánchez-Patán F, Monagas M, Bartolomé B, Moreno-Arribas MV, Peláez C, et al. Effect of grape polyphenols on Lactic acid bacteria and Bifidobacteriagrowth: Resistance and metabolism. Food Microbiol. 2011; 28(7): 1345-52.
  29. Ghorbanzade T, Jafari SM, Akhavan S, Hadavi R. Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food Chem. 2017; 216: 146-52.
  30. Fernandez-Saiz P, Soler C, Lagaron JM, Ocio MJ. Effects of chitosan films on the growth of Listeria monocytogenes, Staphylococcus aureus and Salmonellaspp. In laboratory media and in fish soup. Int J Food Microbiol. 2010; 137(2-3): 287-94.
  31. Salwa A, Galal EA, Neimat E. Carrot yoghurt: Sensory, chemical, microbiological properties and consumer acceptance. Pak J Nutr. 2004; 3(6): 322-30.
  32. Shahbazi Y, Karami N, Shavisi N. Effect of Mentha spicataessential oil on chemical, microbial, and sensory properties of minced camel meat during refrigerated storage. J Food Saf. 2017; 38(1): 1-7.