The Effect of Moderate Intensity Endurance Training with Genistein on Brain- Derived Neurotrophic Factor and Tumor Necrosis Factor-α in Diabetic Rats

Document Type : Research Paper

Authors

1 Department of Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

2 Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran

3 Department of Sport Sciences, Faculty of Literature and Human Sciences, Persian Gulf University, Boushehr, Iran

Abstract

Introduction: As physical education and nutrition are two effective factors in management of diabetes, the purpose of this study was to investigate the effect of moderate intensity endurance training and genistein on serum levels of brain-derived neurotrophic factor (BDNF) and tumor necrosis factor-α (TNF-α) in diabetic rats. Methods: In this experimental study, 40 diabetic rats were selected and divided into five groups: (1) control; (2) moderate intensity endurance training; (3) genistein consumption; (4) moderate intensity endurance training with genistein and (5) sham. Rats in groups 2 and 4 ran on treadmill for eight weeks, three sessions per week, and each session for 60 minutes at speeds of 10- 17 m/min, and rats in groups 3 and 4 received daily 30 mg/kg genistein intraperitoneal injection. Kolmogorov-Smirnov test, independent sample t- test and two-way ANOVA were used to analyze the findings (p≤0.05).
Results: Endurance training had a significant effect on increasing BDNF (P=0.002 and effect size, 0.34) and reducing TNF-α (p = 0.003 and effect size, 0.31), genistein consumption had a significant effect on increasing BDNF (p = 0.01 and effect size, 0.24) and reducing TNF-α (p = 0.001 and effect size, 0.37); also, endurance training with genistein consumption had interactive effects on increasing BDNF (p = 0.04 and effect size, 0.15) and decreasing TNF-α (P = 0.019 and effect size, 0.20). Conclusion: It seems that moderate intensity training and genistein consumption alone can improve the BDNF and TNF-α in diabetic rats; nevertheless simultaneous consumption of genistein can accelerate the effects of moderate intensity training on improvement of BDNF and TNF-α in diabetic rats.

Keywords

References

1.         Lohrasebi M, Edalatmanesh MA, Hosseini SA, Farkhaie F, Salehi M, Kazemi N, et al. Lipid lowering effects of coriandrum sativum extract and endurance training in streptozotocin induced diabetic rats. Rep Health Care. 2016;2(2):31-40.
2.         Salehi OR, Hosseini SA. The Effects of Endurance Trainings on Serum BDNF and Insulin Levels in Streptozotocin-Induced Diabetic Rats. Shefaye Khatam. 2017; 5(2):52-61
3.         Eslami R, Sorkhkamanzadeh G, Kazemi A-R, Gharakhanlou R, Banaifar A-a. Effect of 6-Week Endurance Training on BDNF Expression in Motor Root of Spinal Cord in Rats with Diabetic Neuropathy. J Mazandaran Univ Med Sci. 2015;25(124):94-106.
4.         Banitalebi E, Razavi T, Norian M, Bagheri L. The effect of combined aerobic exercise training and green tea extract on serum TNF-α and IL-6 levels in obese women with type 2 diabetes. Daneshvar Med J. 2016; 11(123): 11-20.
5.         Eskandari M. The Effect of three months aerobic exercise with moderate intensity on serum BDNF and TNF-α in women with metabolic syndrome. Mashhad Med Univ Med Sci. 2016;59(4):242-51. 
6.         Hosseini S, Azarbayjani M. The effect of aqua extract of saffron with resistance training on glycemic indexes of streptozotocin induced diabetic rats. Armaghane Danesh. 2013;18(4):284-94.
7.         Salimi Avansar M. The Effects of Eight Weeks Interval Training and Curcumin Consumption on TNF-α and BDNF Levels in Men with Metabolic Syndrome. J Ardabil Univ Med Sci. 2017;17(3):299-310.
8.         Mostafavi H. The effect of six months aerobic exercise with moderate intensity on BDNF, IL-6, and short-term memory in 50-65 years old women with syndrome metabolic. Yafte. 2017;19(4): 88-101.
9.         Farkhaie F, Hosseini S, Edalat Manesh M. The effect of endurance training with Niglla Sativa extract on lipid profile of diabetic rats. Jahesh J. 2013;14:11-8.
10.       Fallah Huseini H, Fakhrzadeh H, Dastpak A, Azarabadi M, Mohtashami Tokabny R. Review of antihyperlipedemic herbal medicine. J Med Plants. 2005;3(15):9-20.
11.       Azizpour M, Hoveida R, Ahmadyasl N. Evaluating the effect of genistein on the amount of BDNF protein in the spinal cord of diabetic ovariectomized rats. Health Sci. 2016;5(4):228-31.
12.       Yanagisawa M, Sugiya M, Iijima H, Nakagome I, Hirono S, Tsuda T. Genistein and daidzein, typical soy isoflavones, inhibit TNF‐α‐mediated downregulation of adiponectin expression via different mechanisms in 3 T 3‐L 1 adipocytes. Molecul Nutr Food Res. 2012;56(12):1783-93.
13.       Hashemi HS, Hosseini SA. The effect of moderate intensity endurance training and lipid lowering genistein in Streptozotocin induced diabetic rats. J Shahrekord Uuniv Med Sci. 2017;19(1):10-23.
14.       Hosseini SA, Ahmadi M, Sharifi A, Shadmehri S, Zar A. The effect of resistance training and genistein on leptin and lipid profile of streptozotocin-induced diabetic rats. Feyz J. 2018;22(3):248-57.
15.       Hosseini SA, Shojae T, SA. H. The Effect of Cinnamon on Glycemic Index and Insulin Resistance of Streptozotosin Induced Diabetic Rats. Lorestan Univ Med Sci J. 2005;16(4):70-8.
16.       Shojae T, Hosseini S. Reviwe the effect of endurance training on glycemic indexes of streptozotosin induced diabetic rats. Jahesh J. 2013;15:39-46.
17.       Rahmati M, Khazani A, Gharakhanlou R, Movaheddin M, Manaheji H. Chronic effects of moderate intensity endurance training on neuropathic pain symptoms in diabetic rats. Physiol Pharmacol. 2013;16(4):435-45.
18.       Šošic-Jurjević B, Filipović B, Ajdžanović V, Brkić D, Ristić N, Stojanoski MM, et al. A Brife cmmunication: subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle- aged rats. Ex Biol Med. 2007;232(9):1222-7.
19.       Bałkowiec-Iskra E, Vermehren-Schmaedick A, Balkowiec A. Tumor necrosis factor-α increases brain-derived neurotrophic factor expression in trigeminal ganglion neurons in an activity-dependent manner. Neurosci. 2011;180:322-33.
20.       Farzanegi P, MohammadZadeh M. Interactive effect of exercise training with ω-3 supplementation on resting levels of TNF-α and IL-10 in Karat Men. J Hormozgan Univ Med Sci. 2014;18(1):26-34.
21.       Lee SS, Yoo JH, Kang S, Woo JH, Shin KO, Kim KB, et al. The effects of 12 weeks regular aerobic exercise on brain-derived neurotrophic factor and inflammatory factors in juvenile obesity and type 2 diabetes mellitus. J Phys Ther Sci. 2014;26(8):1199-204.
22.       Taheri Chadorneshin H, Afzalpour M, Abtahi H, Foadoddini M. Effect of Intense Exercise Training on Hydrogen Peroxide, Tumor Necrosis Factor-Alpha and the Selected Neurotrophins in Rat’s Brain. Horizon Med Sci. 2015;20(4):231-6.
23.       Arslan M, Ipekci SH, Kebapcilar L, Dogan Dede N, Kurban S, Erbay E, et al. Effect of aerobic exercise training on MDA and TNF-α levels in patients with type 2 diabetes mellitus. Int Scholarly Res Not. 2014; 1-5.
24.       Foadoddini M, Afzalpour ME, TaheriChadorneshin H, Abtahi-Eivary S-H. Effect of Intensive Exercise Training and Vitamin E Supplementation on the Content of Rat Brain Neurotrophic Factors. Iranian Red Crescent Med J. 2018;20(2):e57298. 
25.       Yousefi H, Alihemmati A, Karimi P, Alipour MR, Habibi P, Ahmadiasl N. Effect of genistein on expression of pancreatic SIRT1, inflammatory cytokines and histological changes in ovariectomized diabetic rat. Iranian J Basic Med Sci. 2017;20(4):423.
26.       Pan M, Han H, Zhong C, Geng Q. Effects of genistein and daidzein on hippocampus neuronal cell proliferation and BDNF expression in H19-7 neural cell line. J Nutr Health Aging. 2012;16(4):389-94.
27.       Habibi P, Babri S, Ahmadiasl N, Yousefi H. Effects of genistein and swimming exercise on spatial memory and expression of microRNA 132, BDNF, and IGF-1 genes in the hippocampus of ovariectomized rats. Iranian J Basic Med Sci. 2017;20(8):856.
28.       Imani H, Tabibi H, Atabak S, Rahmani L, Hedayati M, Neyestani T, et al. Effects of soy consumption on serum lipid and apoprotein levels in peritoneal dialysis patients. Iranian J Nutr Sci Food Technol. 2007;2(3):33-44.
29.       Yousefinejad A, Siassi F, Javanbakht MH, Mohammadi H, Ghaedi E, Zarei M, et al. Effect of Genistein and L-carnitine and Their Combination on Lipid Profile and Inflammatory Cytokines in Experimental Nephrotic Syndrome. Rep Biochem Molecul Biol. 2018;7(1):1-8.
30.       Jourkesh M, Choobineh S, Soori R, Ravasi AA. Effect of combined endurance-resistance training and soy extract supplementation on expression of eNOS gene in ovariectomized rats. Arch Med Sci Atheroscler Dis. 2017; 2: 76–81
31.       Orsatti FL, Nahas EAP, Nahas-Neto J, Maesta N, Orsatti CL, Fernandes CE. Effects of resistance training and soy isoflavone on body composition in postmenopausal women. Obstet Gynecol Int. 2010;1-8.
32.       Chilibeck PD, Vatanparast H, Pierson R, Case A, Olatunbosun O, Whiting SJ, et al. Effect of exercise training combined with isoflavone supplementation on bone and lipids in postmenopausal women: a randomized clinical trial. J Bone Miner Res. 2013;28(4):780-93.
33.       Giolo J, Costa J, da Cunha-Junior J, Pajuaba A, Taketomi E, de Souza A, et al. The Effects of Isoflavone Supplementation Plus Combined Exercise on Lipid Levels, and Inflammatory and Oxidative Stress Markers in Postmenopausal Women. Nutr J. 2018;10(4):424.
34.       Bhattamisra S, Mohapatra L, Panda B, Parida S. Effect of isoflavone rich soya seed extract on glucose utilization and endurance capacity in diabetic rat. Diabetol Croat. 2013;42(2):42-53.
Journal of Nutrition,Fasting and Health
Volume 7, Issue 1
March 2019
Pages 44-51

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History

  • Receive Date: 28 December 2018
  • Revise Date: 13 March 2019
  • Accept Date: 13 March 2019

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