Evaluation of the Effect of the Film Prepared on the Basis of Polylactic Acid Tragacanth Gum Containing Origanum Vulgare Essential Oil on the Shelf Life and Characteristics of Feta Cheese

Document Type : Research Paper

Authors

1 Master's Student in Food Hygiene and Quality Control, Amol University of Special Modern Technologies, Amol, Iran.

2 Assistant Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

Abstract

Introduction: Chemical preservatives are widely used to increase the shelf life of food products. But due to their disadvantages, today the main research is in the field of   using natural preservatives. This study was carried out to prepare a film based on polylactic acid and tragacanth gum containing Origanum vulgare essential oil (OVEO) on the microbial, chemical and sensorial properties of feta cheese. Method: OVEO was extracted by steam distillation and its composition was determined by gas chromatography-mass spectrometry (GC-MS). The most important parameters of PLA films were determined as thickness, turbidity and humidity. The antibacterial effects of films were evaluated against four common pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium and Escherichia coli O157:H7) by disk diffusion method. Results: The results of physical properties showed no significant difference in film turbidity when adding different OVEO concentrations (p>0.05). In terms of moisture and turbidity, there was a significant difference between films containing different concentration of OVEO and pure film (p<0.05). The results of the antimicrobial activity showed that the Staphylococcus aureus (10 mm), E. coli (6 mm), Listeria and Salmonella (1 mm), were the most sensitive organisms, respectively. The most sensitive and largest diameter of the inhibition zone was in Aspergillus (13 mm). Conclusion: Based on these findings, the film based on polylactic acid and tragacanth gum containing OVEO can be an effective natural preservative with the least adverse effects on the properties of different products and may have a wide application in food industry. 

Keywords

Main Subjects


  1. Angelopoulou A, Alexandraki V, Georgalaki M, Anastasiou R, Manolopoulou E, Tsakalidou E, et al. Production of probiotic Feta cheese using Propionibacterium freudenreichii subsp. shermanii as adjunct. International Dairy Journal. 2017;1(66):135-9.
  2. Costa MJ, Maciel LC, Teixeira JA, Vicente AA, Cerqueira MA. Use of edible films and coatings in cheese preservation: Opportunities and challenges. Food Research International. 2018;1(107):84-92.
  3. Azizian A, Khanzadi S, Hashemi M, Azizzadeh M. Inhibitory Effect of Nano-gel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia Coli O157:H7 Inoculated in Beef at Cold Storage Condition. Journal of Nutrition, Fasting and Health. 2019;7(2):103-9.
  4. Khanzadi S, Azizian A, Hashemi M, Azizzadeh M. Chemical Composition and Antibacterial Activity of the Emulsion and Nano-emulsion of Ziziphora clinopodioides Essential Oil against Escherichia Coli O157:H7. Journal of Human, Environment and Health Promotion. 2019;5(2):94-7.
  5. Busatta C, Mossi AJ, Rodrigues M.RA, Cansian, RL and Oliveira, JV D. Braz J 2007;1(38):610-6.
  6. Jin TZ. Current state of the art and recent innovations for antimicrobial food packaging. Microbial control and food preservation: Theory and Practice. 2017;1(1):349-72.
  7. War JM, Fazili MA, Mushtaq W, Wani AH, Bhat MY. Role of nanotechnology in crop improvement. Nanobiotechnology in Agriculture: An Approach Towards Sustainability. 2020;1(1):63-97.
  8. Khodaei D, Oltrogge K, Hamidi-Esfahani Z. Preparation and characterization of blended edible films manufactured using gelatin, tragacanth gum and, Persian gum. Lwt. 2020;1(117):108617.
  9. Banuree SA, Noori N, Gandomi H, Khanjari A, Karabagias IK, Faraki A, Ghadami F, Azizian A, Banuree SZ. Effect of Stevia rebaudiana aqueous extract and microencapsulation on the survivability of Bifidobacterium bifidum Bb-12 and Lactobacillus acidophilus La-5 in functional ice cream. International Journal of Food Science & Technology. 2022;57(12):7615-21.
  10. Yadav A, Kumar N, Upadhyay A, Sethi S, Singh A. Edible coating as postharvest management strategy for shelf life extension of fresh tomato (Solanum lycopersicum L.): An overview. Journal of Food Science. 2022;87(6):2256-90.
  11. Lombrea A, Antal D, Ardelean F, Avram S, Pavel IZ, Vlaia L, Mut AM, Diaconeasa Z, Dehelean CA, Soica C, Danciu C. A recent insight regarding the phytochemistry and bioactivity of Origanum vulgare L. essential oil. International journal of molecular sciences. 2020;21(24):9653.
  12. Llana-Ruiz-Cabello M, Pichardo S, Baños A, Núñez C, Bermúdez JM, Guillamón E, Aucejo S, Cameán AM. Characterisation and evaluation of PLA films containing an extract of Allium spp. to be used in the packaging of ready-to-eat salads under controlled atmospheres. LWT-Food Science and Technology. 2015;64(2):1354-61.
  13. Kadkhodai R, Emadzade B, Koochaki A. Evaluation Rheological Behavior of Tragacanth Gum Blend WITH QodoumeShirazi, Farsi and Locust Bean Gums. Journal of Food Science and Technology (Iran). 2016;14(63):141-29.
  14. Mostafavi FS, Kadkhodaee R, Emadzade B, Koochaki A. Preparation and characterization of tragacanth-locust bean gum edible blend films. Carbohydrate polymers. 2016;30(139):20-7.
  15. Rezaeigolestani M, Misaghi A, Khanjari A, Basti AA, Abdulkhani A, Fayazfar Antimicrobial evaluation of novel poly-lactic acid-based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages. International Journal of Food Microbiology. 2017;2(260):1-10.
  16. Bahrami A, Mokarram RR, Khiabani MS, Ghanbarzadeh B, Salehi R. Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles. International Journal of Biological Macromolecules. 2019;15(129):1103-12.
  17. Leelaphiwat P, Pechprankan C, Siripho P, Bumbudsanpharoke N, Harnkarnsujarit N. Effects of nisin and EDTA on morphology and properties of thermoplastic starch and PBAT biodegradable films for meat packaging. Food Chemistry. 2022;1(369):130956.
  18. Hajlaoui H, Snoussi M, Ben Jannet H, Mighri Z, Bakhrouf A. Comparison of chemical composition and antimicrobial activities of Mentha longifolia L.SSP. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid). Annals of Microbiology. 2008;58(10):513-520.
  19. Pourhosseini SH, Mirjalili MH, Nejad Ebrahimi S, Sonboli A. Essential oil quantity and quality of different plant organs from Perovskia abrotanoides Karel in natural habitat of North Khorasan province. Plant Productions. 2018;40(4):53-62.
  20. Janani N, Zare EN, Salimi F, Makvandi P. Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging. Carbohydrate polymers. 2020;1(247):116678.
  21. Azadi A, Rafieian F, Sami M, Rezaei A. Fabrication, characterization and antimicrobial activity of chitosan/tragacanth gum/polyvinyl alcohol composite films incorporated with cinnamon essential oil nanoemulsion. International Journal of Biological Macromolecules. 2023;1(245):125225.
  22. Ojagh SM, Razavi SH, Rezaei M, Hosseini SM, Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food chemistry. 2010;1(12):161-6.
  23. Talebi F, Misaghi A, Khanjari A, Kamkar A, Gandomi H, Saeedi M, Evaluation of antimicrobial activity of poly Lactic Acid (PLA) films containing cellulose nanoparticle and Bunium Persicum and Mentha pepperita essential oils (EOs). Iran J Vet Med. 2017;1(11):289-98.
  24. Ranjbar S, Movahed S, Ahmadi Chenarbin H. The effect of oregano essential oil on the sensory characteristics and shelf life of hamburgers, Food Sciences and Industries. 2016;14(69):33-45.
  25. Khanjari A, Bahonar A, Noori N, Siahkalmahaleh MR, Rezaeigolestani M, Asgharian Z, Khanjari J. In vitro antibacterial activity of Pimpinella animus essential oil and its influence on microbial, chemical, and sensorial properties of minced beef during refrigerated storage. Journal of Food Safety. 2019;39(4):126-26.