Measurement of Heavy Metals in Bread with an Emphasis on the Risk Assessment of Aluminum

Document Type : Research Paper

Authors

1 PhD Student of Animal and Poultry Health and Nutrition, Department of Basic Sciences, Toxicology and Animal Poisoning Research Center, Faculty of Veterinary Medicine, University of Tehran (FVM-UT), Tehran, Iran.

2 Professor of Toxicology, Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran (FVM-UT), Tehran, Iran.

3 PhD Student of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran (FVM-UT), Tehran, Iran.

Abstract

Introduction: Humans are exposed to aluminum (Al) and other heavy metals through various sources. Scientists have long investigated the effects of Al and heavy metals on human health, reporting a correlation between Al concentrations and health issues such as Alzheimer’s diseases and cancer. Therefore, a risk analysis study is required to assess the risk of non-cancerous diseases. The present study aimed to measure heavy metals in bread with an emphasis on the risk assessment of Al. Methods: Various types of flatbread, cakes, and muffins were randomly collected in Tehran, Iran. The samples were prepared based on the modified AOAC official method. Subsequently, test solutions were analyzed for Al, Pb, Hg, Ni, As, Cd, Co, Cr, and Cu via inductively-coupled plasma/optical emission spectrophotometry (ICP-OES). Significant differences between the bread sample groups were determined using one-way analysis of variance (ANOVA) and Duncan’s least square difference (LSD) test. Results: We calculated the concentration, daily intake (DI), national theoretical maximum daily intake (NTMDI), and the hazard quotient (HQ) of Al in Iran. The lowest concentration of Al was observed in Sangak bread, and the highest level was detected in Taftan bread. In addition, the highest Al concentration was observed in cakes (mean: 40.44). The DI of Al was estimated at 0.26 mg/kg, and the NTMDI for adults was 0.005. The HQ of Al in all the bread samples was less than one. Conclusion: According to the results, the mean acceptable daily intake of Al was 92% of the provisional tolerable daily intake, and the HQ was less than one in the studied bread samples. Therefore, no risk of non-cancer diseases was observed due to the consumption of the bread samples.

Keywords

References

  1. Hadian Z, S. Reihani SF, Tan TC, Mousavi Khaneghah A. A study on sodium chloride content in traditional Iranian bread as an approach to control and prevent non-communicable diseases. Int J Environ Anal Chem. 2021 7:1-2.
  2. Naghipour D, Amouei A, Nazmara Sh, A Comparative Evaluation of Heavy Metals in the Different Breads in Iran: A Case Study of Rasht City. Health Scope. 2014;3(4): e18175
  3. Kamalabadi M, Kamankesh M, Mohammadi A, Hadian Z, Ferdowsi R. Contamination and daily intake of polycyclic aromatic hydrocarbons in Iranian bread samples. Polycycl Aromat Compd. 2020;(40)4: 1187-95
  4. Stadler D, Lambertini F, Bueschl C, Wiesenberger G, Hametner C, Schwartz-Zimmermann H, et al. Untargeted LC–MS based 13C labelling provides a full mass balance of deoxynivalenol and its degradation products formed during baking of crackers, biscuits and bread. Food Chem. 2019 1; 279:303-11.
  5. Moret S, Conchione C, Srbinovska A, Lucci P. Microwave-based technique for fast and reliable extraction of organic contaminants from food, with a special focus on hydrocarbon contaminants. Foods. 2019; 8(10):503.
  6. Akpambang VO, Onifade AP. Trace Metals Contamination in Bread Ingredients and Bread from Bakeries in Nigeria. Asian J Appl Chem Res. 2020 23:26-37.
  7. Crisponi G, Fanni D, Gerosa C, Nemolato S, Nurchi VM, Crespo-Alonso M, et al. The meaning of aluminium exposure on human health and aluminium-related diseases. Biomol Concepts. 2013; 4(1):77-87
  8. Yokel RA, Hicks CL, Florence RL. Aluminum bioavailability from basic sodium aluminum phosphate, an approved food additive emulsifying agent, incorporated in cheese. FoodChem Toxicol. 2008; 46(6):2261-6.
  9. Cao P, Liu AD, Yang DJ, Liang J, Wang XD, Xu HB, et al. Assessment of dietary exposure of young Chinese children to aluminium residues. Food Addit Contam: Part A. 2019; 36(4):582-91.
  10. Xu XL, Jiang QJ. Determination and evaluation of aluminum contents in flour products. InApplied Mechanics and Materials. 2012; 138:1022-27. Trans Tech Publications Ltd.
  11. Ghoreishy F, Salehi M, Fallahzade J. Cadmium and lead in rice grains and wheat breads in Isfahan (Iran) and human health risk assessment. Human and Ecological Risk Assessment. 2019; 25(4):924-34.
  12. Nejabat M, Kahe H, Shirani K, Ghorbannezhad P, Hadizadeh F, Karimi Gh. Health risk assessment of heavy metals via dietary intake of wheat in Golestan Province, Iran. Hum Ecol Risk Assess. 2017; 23(5): 1193-201.
  13. Khaniki GR, Yunesian M, Mahvi AH, Nazmara S. Trace metal contaminants in Iranian flat breads. J Agric Soc Sci. 2005; 1(4):301-3.
  14. European Food Safety Authority (EFSA). Safety and efficacy of chromium methionine (Availa® Cr) as feed additive for all species. EFSA Journal. 2009; 7(4):1043.
  15. US Food and Drug, Toxicological Principles for the Safety Assessment of Direct Food Additives and Color Additives Used in Food. Redbook II. Draft Guidance, Chapter 2. 1993.
  16. Institute of Standards and Industrial Research of Iran Food & Feed-Maximum limit of heavy metals. ISIRI no 12968. 1st Edition, Karaj: ISIRI; 12968 [in Persian].
  17. Abdi F, Atarodi Kashani Z, Mirmiran P, Esteki T. Surveying global and Iranian food consumption patterns: A review of the literature.  Journal of Fasa University of Medical Sciences. 2015; 5(2): 159-67.
  18. Ghosouri S, Haghighi A. Iranian family bread consumption during 1386-1392. Statistics. 2015; 3(4):46-52.
  19. Ma N, Liu ZP, Yang DJ, Liang J, Zhu JH, Xu HB, et al. Risk assessment of dietary exposure to aluminum in the Chinese population. Food Addit Contam: Part A. 2016; 33(10):1557-62.
  20. Wong WW, Chung SW, Kwong KP, Yin Ho Y, Xiao Y. Dietary exposure to aluminium of the Hong Kong population. Food Addit Contam. 2010; 27(4):457-63.
  21. Mohaghegh F, Hamta A, Shariatzade M A. The study of cancer incidence and cancer registration in Markazi Province between 2001-2006 and comparison with national statistics, Iran (Persian). J Arak Uni Med Sci. 2008; 11(2):84-93.
  22. Technical Report. Dietary exposure to aluminum-containing food additives European Food Safety Authority. European Food Safety Authority. Parma, Italy. 2013.
  23. MS Q, MS S, AI S. Health risk assessment after exposure to aluminium in drinking water between two different villages. Water Resour Prot. 2011; 3(4): 268-74.

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History

  • Receive Date: 22 October 2021
  • Revise Date: 30 November 2021
  • Accept Date: 12 December 2021

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