The Effect of Endurance Training with Vitamin D on Bone Resorption Markers in Rats Exposed to Oxidative Damage Induced by H2O2

Document Type: Research Paper


1 PhDof exercise physiology, Shoushtar Branch, Islamic Azad University (I.A.U), Shoushtar, Iran

2 Professor of department of Exercise Physiology, Central Tehran Branch, Islamic Azad University (I.A.U), Tehran, Iran.

3 Associate Professor of neurosciences research center, Baqiyatallah University of medical sciences, Tehran, Iran.

4 Assistant professor of department of Physical education & sport sciences , Masjed-Soleiman Branch, Islamic Azad University (I.A.U), Masjed-Soleiman, Iran


Introduction:studies have shown that the increase of active oxygen species is associated with damage to bone cells and the role of exercise and vitamin D on bone health has been proven;the aim of this study was to investigate the interactive effects of aerobic exercise with vitamin D supplements on bone resorption markers in rats exposed to oxidative damage induced by H2O2.
Methods: Sixty male adult male Wistar rats were randomly divided into 10 groups of six rats including:1)1 mmol/kg H2O2, 2)2 mmol/kg H2O2, 3)1mmol/kg H2O2+vitamin D,4) 1mmol/kg H2O2+aerobic training,5)1mmol/kg H2O2+Vitamin D+aerobic training,6)2 mmol/kg H2O2+Vitamin D,7)2mmol/kg H2O2+aerobic training,8)2mmol/kg H2O2+Vitamin D+aerobic training;9)Sham (D-methyl- Sulfoxide+normal saline); and(10)control and for 8 weeks were under the protocol of intervention.The levels of TRACP/5B and NTX were measured by ELISA method. Two-way and one-way ANOVA with Tukey’s post hoc test was used to analysis of data (p≤0.05).
Results: 1and 2 mmol/kg H2O2 had no significant effect on NTX and TRACP/5B levels(p≥0/05). Eight weeks of aerobic training increased of NTX levels in rats exposed to 2mmol/kg H2O2 (p≤0/05).Eight weeks of Vitamine D had no significant effect on NTX and TRACP/5B levels (p≥0/05).Also eight weeks of aerobic training along with Vitamin D had no interactive effects on increase of NTX and TRACP/5B levels in rats exposed to 1and 2mmol/kg H2O2(p≥0/05).
Conclusion: It seems that doses of 1 and 2mg/kg H2O2 have not significant effect on changes in bone resorption markers,but aerobic exercise had a significant effect on NTX increase in rats exposed to oxidative damage induced by 2mg/kg H2O2.


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Articles in Press, Accepted Manuscript
Available Online from 06 August 2019
  • Receive Date: 11 June 2019
  • Accept Date: 06 August 2019