Document Type: Research Paper
PhDof exercise physiology, Shoushtar Branch, Islamic Azad University (I.A.U), Shoushtar, Iran
Professor of department of Exercise Physiology, Central Tehran Branch, Islamic Azad University (I.A.U), Tehran, Iran.
Associate Professor of neurosciences research center, Baqiyatallah University of medical sciences, Tehran, Iran.
Assistant professor of department of Physical education &amp; sport sciences , Masjed-Soleiman Branch, Islamic Azad University (I.A.U), Masjed-Soleiman, Iran
Introduction:studies have shown that the increase of active oxygen species is associated with damage to bone cells and the role of exercise and vitamin D on bone health has been proven;the aim of this study was to investigate the interactive effects of aerobic exercise with vitamin D supplements on bone resorption markers in rats exposed to oxidative damage induced by H2O2.
Methods: Sixty male adult male Wistar rats were randomly divided into 10 groups of six rats including:1)1 mmol/kg H2O2, 2)2 mmol/kg H2O2, 3)1mmol/kg H2O2+vitamin D,4) 1mmol/kg H2O2+aerobic training,5)1mmol/kg H2O2+Vitamin D+aerobic training,6)2 mmol/kg H2O2+Vitamin D,7)2mmol/kg H2O2+aerobic training,8)2mmol/kg H2O2+Vitamin D+aerobic training;9)Sham (D-methyl- Sulfoxide+normal saline); and(10)control and for 8 weeks were under the protocol of intervention.The levels of TRACP/5B and NTX were measured by ELISA method. Two-way and one-way ANOVA with Tukey’s post hoc test was used to analysis of data (p≤0.05).
Results: 1and 2 mmol/kg H2O2 had no significant effect on NTX and TRACP/5B levels(p≥0/05). Eight weeks of aerobic training increased of NTX levels in rats exposed to 2mmol/kg H2O2 (p≤0/05).Eight weeks of Vitamine D had no significant effect on NTX and TRACP/5B levels (p≥0/05).Also eight weeks of aerobic training along with Vitamin D had no interactive effects on increase of NTX and TRACP/5B levels in rats exposed to 1and 2mmol/kg H2O2(p≥0/05).
Conclusion: It seems that doses of 1 and 2mg/kg H2O2 have not significant effect on changes in bone resorption markers,but aerobic exercise had a significant effect on NTX increase in rats exposed to oxidative damage induced by 2mg/kg H2O2.