The Effects of Alginate Coatings Containing Zataria multiflora Boiss Essential Oil in the Forms of Coarse Emulsion and Nano-emulsion on Inoculated Escherichia coli O157: H7 in Beef Fillets

Document Type: Research Paper

Authors

1 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

The present study aimed to compare the inhibitory effects of alginate coatings containing Zataria multiflora Boiss essential oil (ZMEO) in the forms of coarse emulsion and nano-emulsion on the growth of inoculated Escherichia coliO157: H7in beef fillet during 16 days of refrigeration at the temperature of 4 . Alginate solutions (3%) with various concentrations of ZMEO (0.25%, 0.5%, and 1%) were prepared, and the coarse emulsion and nano-emulsion forms were also prepared. The beef fillets were inoculated with E. coliO157: H7(1.5×105 log CFU/g) and immersed in various alginate treatments, and the bacterial count was performed during refrigeration on days zero, four, eight, 12, and 16.The obtained results indicated that the alginate coating containing ZMEO in both forms (coarse/nano-emulsion) was a proper candidate to control E. coliO157: H7at the temperature of 4°C. However, the antibacterial effects were more significant on the samples treated by the nano-emulsion form compared to the coarse emulsion form and controls. In addition, the lowest bacterial growth was observed in the samples coated with the alginate nano-emulsion containing 1% ZMEO (5.3±0.24 log CFU/g) at the end of storage. Therefore, it could be concluded that the use of alginate coatings containing ZMEO (particularly in the nano-emulsion form) could effectively decrease the growth of E. coliO157: H7 during storage, and this natural additive could be applied in the food industry, especially the meat industry.

Keywords


1. Ukuku DO, Zhang H, Huang L. Growth parameters of Escherichia coli O157: H7, Salmonella spp., Listeria monocytogenes, and aerobic mesophilic bacteria of apple cider amended with nisin–EDTA. Foodborne Pathog Dis. 2009; 6(4): 487-94.

2. Raeisi M, Tajik H, Aliakbarlu J, Valipour S. Effect of carboxymethyl cellulose edible coating containing Zataria multiflora essential oil and grape seed extract on chemical attributes of rainbow trout meat. Vet Res Forum. 2014; 5(2): 89-93.

3. Song Y, Liu L, Shen H, You J, Luo Y. Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control. 2011; 22(3-4): 608-15.

4. Berenji Ardest S, Azizi MH, Zohourian G, Hadian Z, Amiri Z. Evaluation of Mechanical and Physical Properties of Calcium-Alginate Edible Films. Quarterly Iranian Journal of Food Science and Technology. 2011; 7(4): 55-64. (Persian)

5. Palou L, Ali A, Fallik E, Romanazzi G. GRAS, plant-and animal-derived compounds as alternatives to conventional fungicides for the control of postharvest diseases of fresh horticultural produce. Postharvest Biol Technol. 2016; 122: 41-52.

6. Rhim JW. Physical and mechanical properties of water resistant sodium alginate films. LWT-Food science and technology. 2004; 37(3): 323-30.

7. Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss.(Shirazi thyme)—an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol. 2013; 145(3): 686-98.

8. Shah P, Bhalodia D, Shelat PK. Nanoemulsion: a pharmaceutical review. Systematic Reviews in Pharmacy. 2010; 1(1): 24-32.

9. Solans C, Izquierdo PJ, Nolla J, Azemar N, Garcia-Celma MJ. Nano-emulsions. Curr Opin Colloid Interface Sci. 2005; 10(3-4): 102-10.

10. Jerobin J, Makwana P, Kumar RSS, Sundaramoorthy R, Mukherjee A, Chandrasekaran N. Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro. Int J Nanomedicine. 2015; 10(Suppl 1): 77-86.

11. McLay JC, Kennedy MJ, O'Rourke AL, Elliot RM, Simmonds RS. Inhibition of bacterial foodborne pathogens by the lactoperoxidase system in combination with monolaurin. I J Food Microbiol. 2002; 73(1): 1-9.

12. Heydari R, Bavandi S, Javadian SR. Effect of sodium alginate coating enriched with horsemint (M entha longifolia) essential oil on the quality of bighead carp fillets during storage at 4° C. Food Sci Nutr. 2015; 3(3): 188-94.

13. Yener FY, Korel F, Yemenicioğlu A. Antimicrobial activity of lactoperoxidase system incorporated into cross‐linked alginate films. J Food Sci. 2009; 74(2): M73-9.