The Assessment the Quality of Probiotic Yogurt Using the Rosmarinus Officinalis Essential Oil

Document Type: Research Paper

Authors

1 Department of Food Research, Standards Organization, Tehran, Iran.

2 Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction: Antimicrobial compounds have recently attracted the attention of researchers across the world. Essential oils (EOs) could be used as potent herbal antimicrobial materials and natural antioxidants in the food industry. The present study aimed to assess the effects of Rosmarinus officinalis essential oil (REO) with variable phenol contents on the properties of probiotic yogurt containing Bifidobacterium bifidumMethods: Yogurt was prepared by adding Lactobacillus bulgaricus and Streptococcus thermophilus as the starter and the Bifidobacterium bifidum as the probiotic strains. REO was also added at the three concentrations of 1%, 2%, and 3%. Titratable acidity, pH, syneresis, and rheological, microbial, and sensory properties were evaluated at three storage times (7, 14, and 21 days). The total phenol content was approximately 8.93±0.11 milligrams of gallic acid. Results: The bacterial activity significantly declined in all the samples during 20 days of storage (P<0.05), and the addition of REO decreased the lowering rate of bacterial growth in the probiotic and starter strains over 21 days. Moreover, refrigerated storage decreased the pH and viscosity (P<0.05), which increasing increased titratable acidity and syneresis (P<0.05). The probiotic yogurt with REO exhibited thixotropic non-Newtonian behavior, and the samples containing 1% REO had the highest scores of flavor, odor, texture, and overall acceptability. Conclusion: According to the results, high levels of phenolic compounds contributed to the biological, physicochemical, and rheological properties of probiotic yogurt, as well as its sensory scores.

Keywords


1. Shahbazi Y, Shavisi N. Effects of Oregano Methanolic Extract on the Chemical, Microbial, and Sensory Properties of Yogurt. Journal of Nutrition Fasting and Health. 2019; 7(3): 138-45.
2.    Ghorban Shiroodi S, Mohammadifar MA, Ghorbani Gorji E, Ezzatpanah H, Zohouri N. Influence of gum tragacanth on the physicochemical and rheological properties of kashk. J Dairy Res. 2012; 79(1): 93-101.
3.    Vanegas-Azuero AM, Gutiérrez LF. Physicochemical and sensory properties of yogurts containing sacha inchi (Plukenetia volubilis L.) seeds and β-glucans from Ganoderma lucidum. J Dairy Sci. 2018; 101(2): 1020-33.
4.    Roesch R, Juneja M, Monagle C, Corredig M. Aggregation of soy/milk mixes during acidification. Food Res Int. 2004; 37(3): 209-15.
5.    Singh M, Kim S, Liu SX. Effect of Purified Oat β‐Glucan on Fermentation of Set‐Style Yogurt Mix. J Food Sci. 2012; 77(8): E195-201.
6.    Williams EB, Hooper B, Spiro A, Stanner S. The contribution of yogurt to nutrient intakes across the life course. Nutr Bull. 2015;40(1): 9-32.
7.    Guarner F, Khan AG, Garisch J, Eliakim R, Gangl A, Thomson A, et al. Probiotics and prebiotics. World Gastroenterology Organisation Global Guidelines; 2011.
8.    Lee YK, Salminen S. Handbook of probiotics and prebiotics. Hoboken: John Wiley & Sons; 2009.
9.    Mitsuoka T, Kaneuchi C. Ecology of the bifidobacteria. Am J Clin Nutr. 1977; 30(11): 1799-810.
10. Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C. Review article: bifidobacteria as probiotic agents–physiological effects and clinical benefits. Aliment Pharmacol Ther. 2005; 22(6): 495-512.
11. Donkor O, Henriksson A, Vasiljevic T, Shah NP. Effect of acidification on the activity of probiotics in yoghurt during cold storage. Int Dairy J. 2006; 16(10): 1181-9.
12. Dave RI, Shah NP. Effectiveness of ascorbic acid as an oxygen scavenger in improving viability of probiotic bacteria in yoghurts made with commercial starter cultures. Int Dairy J. 1997; 7(6-7):435-43.
13. Güler Z, Gürsoy-Balcı AC. Evaluation of volatile compounds and free fatty acids in set types yogurts made of ewes’, goats’ milk and their mixture using two different commercial starter cultures during refrigerated storage. Food Chem. 2011; 127(3): 1065-71.
14. Ghalem BR, Zouaoui B. Evaluation of the quality of steamed yogurt treated by Lavandula and Chamaemelum species essential oils. J  Med Plant Res. 2013; 7(42): 3121-6.
15. Da Silva DF, Tenório Junior NN, Gomes RG, dos Santos Pozza MS, Britten M, Matumoto-Pintro PT. Physical, microbiological and rheological properties of probiotic yogurt supplemented with grape extract. J Food Sci Technol. 2017; 54(6): 1608-15.
16. Al-Otaibi M, El.Demerdash H. Improvement of the quality and shelf life of concentrated yoghurt (labneh) by the addition of some essential oils. Afr J Microbiol Res. 2008; 2(7): 156-61.
17. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol. 2004; 94(3): 223-53.
18. El–Nawawy M, El–Kenany Y, Abd El Ghaffar E. Effect of some herb plants on the use of yoghurt culture. Paper presented at: Annals of Agriculture Science, 7th conference of agriculture research, Ain Shams university of Cairo, Egypt; 15–17 December 2008.
19. Golestan L, Seyedyousefi L, Kaboosi H, Safari H. Effect of Mentha spicata L. and M entha aquatica L. essential oils on the microbiological properties of fermented dairy product, kashk. Int J Food Sci Technol. 2016; 51(3): 581-7.
20. Yangilar F, Yildiz PO. Effects of using combined essential oils on quality parameters of bio‐yogurt: Yangilar and Yildiz. J Food Process  Preserv. 2017; 42(2): e13332.
21. Genena AK, Hense H, Smânia Junior A, Souza SM. Rosemary (Rosmarinus officinalis): a study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbon dioxide. Food Sci Technol. 2008; 28(2): 463-9.
22. Hussain AI, Anwar F, Chatha SAS, Jabbar A, Mahboob S, Nigam PS. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities. Braz J Microbiol. 2010; 41(4): 1070-8.
23. AOAC. Official methods of analysis, 16th ed. Association of official analytical chemists, arlington, va, USA. 1995.
24. Amatayakul T. The improvement of physical properties of yoghurts by varying casein/whey protein ratio and EPS-producing starter cultures, Victoria University; 2005.
25. Karsheva M, Paskov V, Tropcheva R, Georgieva R, Danova S. Physicochemical parameters and rheological properties of yoghurts during the storage. Journal of Chemical Technology and Metallurgy. 2013; 48(5): 483-8.
26. Vinderola CG, Reinheimer JA. Culture media for the enumeration of Bifidobacterium bifidum and Lactobacillus acidophilus in the presence of yoghurt bacteria. Int Dairy J. 1999; 9(8): 497-505.
27. Eissa EA, Yagoub AEA, Babiker EE, Mohamed Ahmed IA. Physicochemical, microbiological and sensory characteristics of yoghurt produced from camel milk during storage. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2011; 10(6): 2305-13.
28. Bajalan I, Rouzbahani R, Ghasemi Pirbalouti A, Maggi F. Quali-quantitative variation of essential oil from Iranian rosemary (Rosmarinus officinalis L.) accessions according to environmental factors. Journal of Essential Oil Research. 2018; 30(1): 16-24.
29. Jaimand K, Rezaee MB, Homami S. Comparison Extraction Methods of Essential oils of Rosmarinus officinalis L. in Iran By Microwave Assisted Water Distillation; Water Distillation and Steam Distillation. Journal of Medicinal Plants and By-products. 2018; 7(1): 9-14.
30. Jalali-Heravi M, Moazeni RS, Sereshti H. Analysis of Iranian rosemary essential oil: Application of gas chromatography–mass spectrometry combined with chemometrics. J Chromatogr A. 2011; 1218(18): 2569-76.
31. Sarabi-Jamab M, Niazmand R. Effect of essential oil of Mentha piperita and Ziziphora clinopodioides on Lactobacillus acidophilus activity as bioyoghurt starter culture. American-Eurasian Journal of Agricultural and Environmental Science. 2009; 6(2): 129-31.
32. Jimborean MA, Salanta LC, Tofana M, Pop CR, Rotar AM, Fetti V. Use of essential oils from citrus sinensis in the development of new type of yogurt. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Food Science and Technology. 2016; 73(1): 24-27.
33. Sun-Waterhouse D, Zhou J, Wadhwa SS. Drinking yoghurts with berry polyphenols added before and after fermentation. Food Control. 2013; 32(2): 450-60.
34. Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala T, et al. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int J Food Microbiol. 2000; 56(1): 3-12.
35. Singh HP, Batish DR, Kaur S, Arora K, Kohli RK. α-Pinene Inhibits Growth and Induces Oxidative Stress in Roots. Ann Bot. 2006; 98(6): 1261-9.
36. Illupapalayam VV, Smith SC, Gamlath S. Consumer acceptability and antioxidant potential of probiotic-yogurt with spices. LWT - Food science and technology. 2014; 55(1): 255-62.
37. Feniman Moritz CM, Mores Rall VL, Saeki MJ, Junior AF. Inhibitory effect of essential oils against Lactobacillus rhamnosus and starter culture in fermented milk during its shelf-life period. Braz J Microbiol. 2012; 43(3): 1147-56.
38. Joung JY, Lee JY, Ha YS, Shin YK, Kim Y, Kim SH, et al. Enhanced Microbial, Functional and Sensory Properties of Herbal Yogurt Fermented with Korean Traditional Plant Extracts. Korean J Food Sci Anim Resour. 2016; 36(1): 90-9.
39. Robinson RK. Yogurt types and manufacture. London, UK: Academic Press; 2002.
40. Shahdadi F, Mirzaie H, Kashaninejad M, Khomeiri M, Ziaiifar AM, Akbarian A. Effects of various essential oils on chemical and sensory characteristics and activity of probiotic bacteria in drinking yoghurt. Agricultural Communications. 2015; 3(1): 16-21.
41. Matumoto-Pintro P, Rabiey L, Robitaille G, Britten M. Use of modified whey protein in yoghurt formulations. Int Dairy J. 2011; 21(1): 21-6.
42. Gürsoy A, Durlu-Özkaya F, Yildiz F, Aslim B. Set type yoghurt production by exopolysaccharide producing turkish origin domestic strains of Streptococcus thermophilus (W22) and Lactobacillus delbrueckii ssp. bulgaricus (B3). Kafkas Universitesi Veteriner Facultesi Dergisi. 2010; 16: S81-6.
43. Lucey JA, Singh H. Formation and physical properties of acid milk gels: a review. Food Res Int. 1997; 30(7): 529-42.
44. Panesar PS, Shinde C. Effect of Storage on Syneresis, pH, Lactobacillus acidophilus Count, Bifidobacterium bifidum Count of Aloe vera Fortified Probiotic Yoghurt. Current Research in Dairy Sciences. 2012; 4(1): 17-23.
45. Han J, Britten M, St-Gelais D, Champagne CP, Fustier P, Salmieri S, et al. Polyphenolic compounds as functional ingredients in cheese. Food Chem. 2011; 124(4): 1589-94.
46. Pereira EPR, Cavalcanti RN, Esmerino EA, Silva R, Guerreiro LRM, Cunha RL, et al. Effect of incorporation of antioxidants on the chemical, rheological, and sensory properties of probiotic petit suisse cheese. J Dairy Sci. 2016; 99(3): 1762-72.
47. Bourne M. Food texture and viscosity: concept and measurement. 2nd Edition. Amsterdam: Elsevier Science & Technology Books; 2002.
48. Cerretani L, Salvador MD, Bendini A, Fregapane G. Relationship between sensory evaluation performed by Italian and Spanish official panels and volatile and phenolic profiles of virgin olive oils. Chem Percept. 2008; 1(4): 258-67.
49. Tarakci Z. Influence of kiwi marmalade on the rheology characteristics, color values and sensorial acceptability of fruit yogurt. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(2): 173-8.
50. Nazaria M, Ghanbarzadeh B, Samadi Kafil H, Zeinalie K, Hamishehkar H. Garlic essential oil nanophytosomes as a natural food preservative: Its application in yogurt as food model.Colloid  Interface Sci Commun. 2019; 30: 20-9.
51. Feniman Moritz CM,  Mores Rall VL, Júri Saeki M, Júnior AF. Assessment of antimicrobial activity of cinnamon (Cinnamomum zeylanicum) essential oil in yogurt. Acta Scientiarum. Technology. 2015; 37(1): 12-26.
52. Mahmoudi R, Gajarbeygi P, Mahmodzadeh F, Hassanzadeh M, Kiyania R, Asadi Nadari MR. Quality of yogurt blended with thymus kotschyanus essential oil. Malaysian Journal of Science. 2014; 33(2): 176-82.
53. Ghalem BR, Zouaoui B. Evaluation of the quality of steamed yogurt treated by Lavandula and Chamaemelum species essential oils. Journal of Medicinal Plants Research. 2013: 7(42): 3129-34.
54. Singh G, Singh Kapoor IP, Singh P. Effect of volatile oil and oleoresin of anise on the shelf life of yogurt. J Food Process Preserv. 2011; 35(6): 778-83.