The Combined Effect of Shallot Extract by Vacuum Rotary Evaporator Technique with Common Antibiotics Against Multi-Drug-Resistant Bacteria Using the Checkerboard Technique

Document Type: Research Paper

Authors

1 Department of Medical Laboratory Sciences, Varastegan Institute of Medical Sciences, Mashhad, Iran.

2 department of nutrition sciences, varastegan institute for medical sciences, Mashhad, Iran

10.22038/jnfh.2020.47566.1257

Abstract

Introduction: Allium hirtifolium (Persian shallot) belongs to the Alliaceae family. Recently, the ethanolic extract of Persian shallot was shown to have significant activity against some of the important clinical pathogens. This study has been assessed the in vitro antibacterial potency of ethanolic extracts of Persian shallot (Iranian Moosir) combined with common antibiotics against five clinically important antibiotic-resistant pathogens. Methods: Antibacterial activities determined through disk diffusion. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated by the broth microdilution method for the extract and selected antibiotics. Finally, the checkerboard technique applied for evaluation of the combined effect of the extract and antibiotics. Results: The MICs of the extract and antibiotics ranged from 4 to 16 mg/mL and 4 to 128 µg/mL, respectively. The MBCs of the extract and antibiotics varied from 8 to 16 mg/mL and 8 to 128 µg/mL, respectively. The results of the checkerboard technique showed that Amikacin and Trimethoprim/sulfamethoxazole have synergistic effects, but Levofloxacin, Imipenem, and Vancomycin have antagonistic impacts in combination with the extract on all isolates. ConclusionsIt is suggested by the findings of this study which the in vitro application of Persian shallot extract combined with Amikacin and Trimethoprim/sulfamethoxazole. The mentioned combinations can inhibit the growth of five clinically significant antibiotic-resistant pathogens effectively.

Keywords


1. Spellberg B, Gilbert DN. The future of antibiotics and resistance: a tribute to a career of leadership by John Bartlett. Clinical infectious diseases. 2014;59(suppl_2):S71-S5.
2. Ventola CL. The Antibiotic Resistance Crisis: Part 1: Causes and Threats. Pharmacy and Therapeutics. 2015;40(4):277-83.
3. Control CfD, Prevention. Office of Infectious Disease. Antibiotic resistance threats in the United States, 2013. April 2013. Accessed January. 2015;28.
4. Gould IM, Bal AM. New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013;4(2):185-91.
5. Rios J, Recio M. Medicinal plants and antimicrobial activity. Journal of ethnopharmacology. 2005;100(1-2):80-4.
6. Ismail S, Jalilian FA, Talebpour AH, Zargar M, Shameli K, Sekawi Z, et al. Chemical Composition and Antibacterial and Cytotoxic Activities of Allium hirtifolium Boiss. BioMed Research International. 2013;2013:696835.
7. Ali SE, Chehri K, Karimi N, Karimi I. Computational approaches to the in vitro antibacterial activity of Allium hirtifolium Boiss against gentamicin-resistant Escherichia coli: focus on ribosome recycling factor. In Silico Pharmacology. 2017;5:7.
8. Ebrahimi R, Zamani Z, Kashi A. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Scientia Horticulturae. 2009;119(4):345-51.
9. Behbahani BA, Fooladi AAI. Evaluation of phytochemical analysis and antimicrobial activities Allium essential oil against the growth of some microbial pathogens. Microbial pathogenesis. 2018;114:299-303.
10. Asgarpanah J, Ghanizadeh B. Pharmacologic and medicinal properties of Allium hirtifolium Boiss. African Journal of Pharmacy and Pharmacology. 2012;6(25):1809-14.
11. Ghahremani-majd H, Dashti F, Dastan D, Mumivand H, Hadian J, Esna-Ashari M. Antioxidant and antimicrobial activities of Iranian mooseer (Allium hirtifolium Boiss) populations. Horticulture, Environment, and Biotechnology. 2012;53(2):116-22.
12. Diba A, Alizadeh F. In vitro and in vivo antifungal activity of Allium hirtifolium and Allium sativum. Avicenna Journal of Phytomedicine. 2018;8(5):465-74.
13. Soroush S, Taherikalani M, Asadollahi P, Asadollahi K, Taran M, Emaneini M, et al. In vitro antimicrobial activity of Persian shallot (Allium hirtifolium). Roum Arch Microbiol Immunol. 2012;71(2):70-4.
14. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. CLSI supplement M100. Wayne PCaLSI.
15. Mandal S, Mandal M, Pal N. Evaluation of combination effect of ciprofloxacin and cefazolin against Salmonella enteric serovar typhi isolates by in vitro methods. Calicut Med J. 2004;2(2):e2.
16. Akinyele TA, Igbinosa EO, Akinpelu DA, Okoh AI. In vitro assessment of the synergism between extracts of Cocos nucifera husk and some standard antibiotics. Asian Pacific Journal of Tropical Biomedicine. 2017;7(4):306-13.
17. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis. 2016;6(2):71-9.
18. Moussaoui F, Alaoui T. Evaluation of antibacterial activity and synergistic effect between antibiotic and the essential oils of some medicinal plants. Asian Pacific Journal of Tropical Biomedicine. 2016;6(1):32-7.
19. Fateh R, Nasiri Kashani MJ, Motevallian M, Falahati M, Yazdanparast A. In vitro antifungal activity of Allium hirtifolium in comparison with miconazole. Medical Journal of The Islamic Republic of Iran (MJIRI). 2010;24(1):17-22.
20. Sharifi-Rad J, Mnayer D, Tabanelli G, Stojanović-Radić Z, Sharifi-Rad M, Yousaf Z, et al. Plants of the genus Allium as antibacterial agents: From tradition to pharmacy. Cellular and Molecular Biology. 2016;62(9):57-68.
21. Moore D. Antibiotic Classification & Mechanism. Retrieved August. 2015;24.
22. Karunanidhi A, Ghaznavi-Rad E, Hamat RA, Pichika MR, Lung LTT, Mohd Fauzi F, et al. Antibacterial and Antibiofilm Activities of Nonpolar Extracts of Allium stipitatum Regel. against Multidrug Resistant Bacteria. BioMed research international. 2018;2018.
23. Safari E, Amiri M, Bahador A, Amiri M, Esmaeili D. The study of antibacterial effects of alcoholic extracts of Arum maculatum, Allium hirtifolium and Teucrium polium against nosocomial resistance bacteria. Int J Curr Microbiol App Sci. 2014;3(2):601-5.
24. Amin M, Kapadnis B. Heat stable antimicrobial activity of Allium ascalonicum against bacteria and fungi. 2005.
25. Mikaili P, Maadirad S, Moloudizargari M, Aghajanshakeri S, Sarahroodi S. Therapeutic Uses and Pharmacological Properties of Garlic, Shallot, and Their Biologically Active Compounds. Iranian Journal of Basic Medical Sciences. 2013;16(10):1031-48.