Effects of Green Tea Extract on Ox-LDL and Homocysteine Levels after Resistance Exercise in Obese Men

Document Type : Research Paper

Authors

Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran.

Abstract

Introduction: Green tea is a well-known source of polyphenol catechins, which possess strong antioxidant properties. However, the impact of green tea polyphenol catechins on biological markers of atherosclerosis, namely oxidized low-density lipoprotein (ox-LDL) and homocysteine (Hcy), following resistance exercise (RE), has not been studied in obese individuals. Methods: In this study, ten obese untrained men (age 43-45 y, BMI 32-33) participated voluntarily. They were randomly assigned to receive either green tea extract (GTE) capsules (two capsules of 500 mg per day) or placebo (PL) capsules (two capsules of 500 mg per day maltodextrin) in a double-blind, placebo-controlled crossover design. The supplementation period lasted for two weeks, followed by a two-week washout period. Afterward, the participants performed a RE protocol at 75% of their one-repetition maximum (1RM). Blood samples were collected before and after the RE session to measure the serum concentrations of Hcy and ox-LDL. Results: In the placebo condition, there was a significant increase in serum Hcy and ox-LDL levels from pre- to post-RE. However, GTE supplementation mitigated the exercise-induced rise in serum Hcy and ox-LDL concentrations in obese men. Conclusion: These findings suggest that a two-week supplementation of GTE may offer protection against exercise-induced elevation of Hcy and ox-LDL levels in obese men.

Keywords

Main Subjects


  1. Padwal RS. Obesity, diabetes, and the metabolic syndrome: the global scourge. Canadian Journal of Cardiology. 2014; 30(5):467-72.
  2. Zhang P, Xu X, Li X. Cardiovascular diseases: oxidative damage and antioxidant protection. Eur Rev Med Pharmacol Sci. 2014; 18(20):3091-6.
  3. Huang C-J, McAllister MJ, Slusher AL, Webb HE, Mock JT, Acevedo EO. Obesity-related oxidative stress: the impact of physical activity and diet manipulation. Sports Medicine-Open. 2015; 1(1):32.
  4. Tsutsui T, Tsutamoto T, Wada A, Maeda K, Mabuchi N, Hayashi M, Ohnishi M, Kinoshita M. Plasma oxidized low-density lipoprotein as a prognostic predictor in patients with chronic congestive heart failure. Journal of the American College of Cardiology. 2002; 39(6):957-62.
  5. Toshima S-i, Hasegawa A, Kurabayashi M, Itabe H, Takano T, Sugano J, Shimamura K, Kimura J, Michishita I, Suzuki T: Circulating oxidized low density lipoprotein levels: a biochemical risk marker for coronary heart disease. Arteriosclerosis, Thrombosis, and Vascular Biology. 2000; 20(10):2243-7.
  6. Joubert LM, Manore MM. Exercise, nutrition, and homocysteine. International journal of sport nutrition and exercise metabolism 2006; 16(4):341-61.
  7. Fogelman AM, Shechter I, Seager J, Hokom M, Child JS, Edwards PA. Malondialdehyde alteration of low density lipoproteins leads to cholesteryl ester accumulation in human monocyte-macrophages. Proceedings of the National Academy of Sciences. 1980; 77(4):2214-8.
  8. Stanton LW, White RT, Bryant CM, Protter AA, Endemann G. A macrophage Fc receptor for IgG is also a receptor for oxidized low density lipoprotein. Journal of Biological Chemistry. 1992; 267(31):22446-51.
  9. Brustolin S, Giugliani R, Félix T. Genetics of homocysteine metabolism and associated disorders. Brazilian Journal of Medical and Biological Research 2010, 43(1):1-7.
  10. Neves LB, Macedo DM, Lopes AC: Homocysteine. Jornal Brasileiro de Patologia e Medicina Laboratorial 2004, 40(5):311-20.
  11. König D, Bisse E, Deibert P, Müller H-M, Wieland H, Berg A. Influence of training volume and acute physical exercise on the homocysteine levels in endurance-trained men: interactions with plasma folate and vitamin B12. Annals of nutrition and metabolism. 2003, 47(3-4):114-8.
  12. Pfanzagl B, Tribl F, Koller E, Möslinger T. Homocysteine strongly enhances metal-catalyzed LDL oxidation in the presence of cystine and cysteine. Atherosclerosis. 2003; 168(1):39-48.
  13. Boushey CJ, Beresford SA, Omenn GS, Motulsky AG: A quantitative assessment of plasma homocysteine as a risk factor for vascular disease: probable benefits of increasing folic acid intakes. Jama. 1995; 274(13):1049-57.
  14. Deminice R, Ribeiro DF, Frajacomo FTT. The effects of acute exercise and exercise training on plasma homocysteine: a meta-analysis. PloS one. 2016; 11(3):e0151653.
  15. e Silva AdS, da Mota MPG. Effects of physical activity and training programs on plasma homocysteine levels: a systematic review. Amino acids. 2014; 46(8):1795-804.
  16. Bizheh N, Gharahcholo L. The response of homocysteine and insulin resistance to a single circuit resistance exercise in overweight women. Journal of Shahrekord University of Medical Sciences. 2013; 15(3):9-17.
  17. Bizheh N, Jaafari M, The effect of a single bout circuit resistance exercise on homocysteine, hs-CRP and fibrinogen in sedentary middle aged men. Iranian Journal of Basic Medical Sciences. 2011; 14(6):568.
  18. Kosola J, Ahotupa M, KyrÖlÄinen H, Santtila M, Vasankari T. Good aerobic or muscular fitness protects overweight men from elevated oxidized LDL. Medicine & Science in Sports & Exercise. 2012; 44(4):563-8.
  19. Chisolm GM, Hazen SL, Fox PL, Cathcart MK. The oxidation of lipoproteins by monocytes-macrophages biochemical and biological mechanisms. Journal of Biological Chemistry. 1999; 274(37):25959-62.
  20. Bocan TM, Mueller SB, Brown EQ, Uhlendorf PD, Mazur MJ, Newton RS. Antiatherosclerotic effects of antioxidants are lesion-specific when evaluated in hypercholesterolemic New Zealand white rabbits. Experimental and molecular pathology. 1992; 57(1):70-83.
  21. Shaish A, Daugherty A, O'sullivan F, Schonfeld G, Heinecke JW: Beta-carotene inhibits atherosclerosis in hypercholesterolemic rabbits. The Journal of Clinical Investigation. 1995; 96(4):2075-82.
  22. Jalali F, Hajian TK, Pouramir M, Farzadi M. The Effects of Green Tea on Serum Lipids, Antioxidants, and Coagulation Tests in Stable Coronary Artery Disease: A Prospective Interventional Study. 2008.
  23. Suzuki K, Takahashi M, Li C-Y, Lin S-P, Tomari M, Shing CM, Fang S-H. The acute effects of green tea and carbohydrate coingestion on systemic inflammation and oxidative stress during sprint cycling. Applied Physiology, Nutrition, and Metabolism. 2015; 40(10):997-1003.
  24. Forney GB, Morré DJ, Morré DM. Oxidative stress reduced by a green tea concentrate and capsicum combination: synergistic effects. Journal of Dietary Supplements. 2013; 10(4):318-24.
  25. Miura S, Watanabe J, Sano M, Tomita T, Osawa T, Hara Y, Tomita I. Effects of various natural antioxidants on the Cu2+-mediated oxidative modification of low density lipoprotein. Biological and Pharmaceutical Bulletin 1995, 18(1):1-4.
  26. Tinahones F, Rubio M, Garrido-Sanchez L, Ruiz C, Gordillo E, Cabrerizo L, Cardona F: Green tea reduces LDL oxidability and improves vascular function. Journal of the American College of Nutrition 2008, 27(2):209-213.
  27. Erba D, Riso P, Bordoni A, Foti P, Biagi PL, Testolin G. Effectiveness of moderate green tea consumption on antioxidative status and plasma lipid profile in humans. The Journal of Nutritional Biochemistry. 2005; 16(3):144-9.
  28. Rahimi R, Falahi Z. Effect of green tea extract on exercise-induced oxidative stress in obese men: a randomized, double-blind, placebo-controlled, crossover study. Asian Journal of Sports Medicine. 2017;8(2).
  29. Vincent HK, Morgan JW, Vincent KR: Obesity exacerbates oxidative stress levels after acute exercise. Medicine and science in sports and exercise 2004, 36(5):772-9.
  30. Wald DS, Law M, Morris JK: Homocysteine and cardiovascular disease: evidence on causality from a meta-analysis. BMJ. 2002; 325(7374):1202.
  31. Iglesias-Gutierrez E, Egan B, Díaz-Martínez ÁE, Penalvo JL, Gonzalez-Medina A, Martínez-Camblor P, O’Gorman DJ, Ubeda N. Transient increase in homocysteine but not hyperhomocysteinemia during acute exercise at different intensities in sedentary individuals. PloS One. 2012; 7(12):e51185.
  32. De Crée C, Whiting PH, Cole H. Interactions between homocyst (e) ine and nitric oxide during acute submaximal exercise in adult males. International Journal of Sports Medicine. 2000; 21(04):256-2.
  33. De Crée C, Lane NP, Whiting PH, Cole H: Interactions Between Homocyst (e) ine And Nitric Oxide During Acute Submaximal Exercise In Adult Males. Medicine & Science in Sports & Exercise. 1999; 31(5):S163.
  34. Wright M, Francis K, Cornwell P. Effect of acute exercise on plasma homocysteine. The Journal of sports medicine and physical fitness. 1998; 38(3):262-5.
  35. Gelecek N, Teoman N, Ozdirenc M, Pınar L, Akan P, Bediz C, Kozan O: Influences of acute and chronic aerobic exercise on the plasma homocysteine level. Annals of Nutrition and Metabolism. 2007; 51(1):53-8.
  36. Herrmann M, Schorr H, Obeid R, Scharhag J, Urhausen A, Kindermann W, Herrmann W. Homocysteine increases during endurance exercise. Clinical Chemistry and Laboratory Medicine. 2003; 41(11):1518-24.
  37. Gaume V, Mougin F, Figard H, Simon-Rigaud M, N’guyen U, Callier J, Kantelip J, Berthelot A: Physical training decreases total plasma homocysteine and cysteine in middle-aged subjects. Annals of nutrition and metabolism. 2005; 49(2):125-31.
  38. Jeukendrup A. Modulation of carbohydrate and fat utilization by diet, exercise and environment. In. Portland Press Limited. 2003.
  39. Venta R, Cruz E, ValcÁrcel G, Terrados N. Plasma vitamins, amino acids, and renal function in postexercise hyperhomocysteinemia. Medicine and science in Sports and Exercise. 2009; 41(8):1645-51.
  40. Wang Z, Pini M, Yao T, Zhou Z, Sun C, Fantuzzi G, Song Z. Homocysteine suppresses lipolysis in adipocytes by activating the AMPK pathway. American Journal of Physiology-Endocrinology and Metabolism. 2011; 301(4):E703-12.
  41. Selhub J. Homocysteine metabolism. Annual review of nutrition. 1999; 19(1):217-46.
  42. Chen T-S, Lui C, Smith CH. Folacin content of tea. Journal of the American Dietetic Association. 1983; 82(6):627-32.
  43. Yang T, Koo M. Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation. Atherosclerosis. 2000; 148(1):67-73.
  44. Witztum JL, Steinberg D. Role of oxidized low density lipoprotein in atherogenesis. The Journal of clinical investigation. 1991; 88(6):1785-92.
  45. Jialal I, Fuller CJ, Huet BA. The effect of α-tocopherol supplementation on LDL oxidation: A dose-response study. Arteriosclerosis, thrombosis, and vascular biology. 1995; 15(2):190-8.
  46. Jialal L, Grundy S. Effect of combined supplementation with [alpha]-tocopherol, ascorbate and beta carotene on low-density lipoprotein oxidation. The Endocrinologist. 1994; 4(3):226.
  47. Folcik V, Nivar-Aristy R, Krajewski L, Cathcart M. Lipoxygenase contributes to the oxidation of lipids in human atherosclerotic plaques. The Journal of Clinical Investigation. 1995; 96(1):504-10.
  48. Bailey JM, Makheja A, Lee R, Simon T. Systemic activation of 15-lipoxygenase in heart, lung, and vascular tissues by hypercholesterolemia: relationship to lipoprotein oxidation and atherogenesis. Atherosclerosis. 1995; 113(2):247-58.
  49. Ho C-T, Chen Q, Shi H, Zhang K-Q, Rosen RT. Antioxidative effect of polyphenol extract prepared from various Chinese teas. Preventive Medicine. 1992; 21(4):520-5.
  50. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Riceevans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Archives of Biochemistry and Biophysics. 1995; 322(2):339-46.
  51. Sung H, Min W-K, Lee W, Chun S, Park H, Lee Y-W, Jang S, Lee D-H. The effects of green tea ingestion over four weeks on atherosclerotic markers. Annals of clinical biochemistry. 2005; 42(4):292-7.
  52. Ji LL. Free radicals and antioxidants in exercise and sports. Exercise and Sport Science. 2000:299-317.
  53. Sanchez-Quesada J, Homs-Serradesanferm R, Serrat-Serrat J, Serra-Grima J, Gonzalez-Sastre F, Ordonez-Llanos J. Increase of LDL susceptibility to oxidation occurring after intense, long duration aerobic exercise. Atherosclerosis. 1995; 118(2):297-305.
  54. Wetzstein CJ, Shern-Brewer RA, Santanam N, Green NR, White-Welkley JE, Parthasarathy S. Does acute exercise affect the susceptibility of low density lipoprotein to oxidation?. Free Radical Biology and Medicine. 1998; 24(4):679-82.
  55. Nishimura S, Akagi M, Yoshida K, Hayakawa S, Sawamura T, Munakata H, Hamanishi C. Oxidized low-density lipoprotein (ox-LDL) binding to lectin-like ox-LDL receptor-1 (LOX-1) in cultured bovine articular chondrocytes increases production of intracellular reactive oxygen species (ROS) resulting in the activation of NF-κB. Osteoarthritis and cartilage. 2004; 12(7):568-76.
  56. Wang G-P, Deng Z-D, Ni J, Qu Z-L. Oxidized low density lipoprotein and very low density lipoprotein enhance expression of monocyte chemoattractant protein-1 in rabbit peritoneal exudate macrophages. Atherosclerosis. 1997; 133(1):31-6.
  57. Li D, Mehta JL: Antisense to LOX-1 inhibits oxidized LDL–mediated upregulation of monocyte chemoattractant protein-1 and monocyte adhesion to human coronary artery endothelial cells. Circulation. 2000; 101(25):2889-95.