Cell Fasting: Cellular Response and Application of Serum Starvation

Document Type: Research Paper

Authors

1. Medical Genetics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

 Humans suffer transient or persistent starvation due to a lack of food intake, either because of fasting, voluntary dieting, or due to the scarcity of available food. At the cellular level it is possible to possess pathological starvation during ischemia and solid tumors. Blood provides many nutrients to our cells, and researchers provide these nutrients to cells in culture in the form of enriched culture medium plus serum from animal sources. In response to starvation, animals use hormonal cues to mobilize stored resources to provide nutrients to individual cells. Besides whole-body responses to nutrient deprivation, individual cells sense and react to lack of nutrients. At the cellular level, starvation triggers different responses such as cell cycle arrest and apoptosis. Stop cycling for proliferating cells is the primary response to nutrient deprivation. Under certain conditions, the cell reacts to nutrient deprivation by engaging the mitochondrial pathway of apoptosis. Thus, serum starvation is regarded as a procedure to prepare cells for an experiment in serum-free conditions such as induction cell cycle synchronization. Several researchers have used serum starvation as a tool to study molecular mechanisms involved in different cellular process, metabolic researches and evaluation of a drug effect.
 

Keywords


  1. Caro-Maldonado A, & Muñoz-Pinedo C. Dying for Something to Eat: How Cells Respond to Starvation. The Open Cell Signaling Journal. 2011;3:42-51.
  2. Keyvani V, Kerachian MA. The Effect of Fasting on the Important Molecular Mechanisms Related to Cancer Treatment. J Fasting Health. 2014;2(3):113- 118.
  3. Braun F, Bertin-Ciftci J, et al. Serum-Nutrient Starvation Induces Cell Death Mediated by Bax and Puma That Is Counteracted by p21 and Unmasked by Bcl-xL Inhibition.PLoS ONE. 2011;6(8).
  4. Kulkarni GV, & McCulloch CAG. Serum deprivation induces apoptotic cell death in a subset of Balb/c 3T3 Fibroblasts. J Cell Sci.1994;107, 1169-1179.
  5. Pirkmajer S, & Chibalin AV. Serum starvation: caveat emptor. Am J Physiol Cell Physiol. 2011;301:C272–C279.
  6. Chen M, & Huang J, et al. Serum Starvation Induced Cell Cycle Synchronization Facilitates Human Somatic Cells Reprogramming. PLoS ONE.2012;7(4).
  7. Epstein D, Elias-Bishko S, et al. Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cells.Biochemistry. 1975;14: 5199–5204.
  8. Chao W, Shen Y, et al. Lipopolysaccharide improves cardiomyocyte survival and function after serum deprivation. J Biol Chem. 2005;280: 21997–22005.
  9. Ching JK, Rajguru P ,et al. A role for AMPK in increased insulin action after serum starvation. Am J Physiol Cell Physiol. 2010;299 C1171–C1179.
  10. Kerachian MA, Cournoyer D, et al. Effect of high-dose dexamethasone on endothelial haemostatic gene expression and neutrophil adhesion. J Steroid Biochem Mol Biol. 2009; 116:127-133.