Advancement in Electrochemical DNA-biosensors for GMOs Detection: A review

Document Type : Review Article


1 Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Nutritional Sciences Department, School of Nutritional Sciences and Food Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran.

3 Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences (MUMS), Paradise Daneshgah, Azadi Square, Mashad, Iran

4 Department of Health Education and Health Promotion, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Medical School, University of Aberdeen, Foresterhill, Aberdeen, UK.


Genetically modified organisms (GMOs) are plants or animals whose genetic make-up has been transformed by recombinant DNA technology, which has new features such as resistance to herbicides, virus and insect. Recently, genetic modification of food products has increased in order to reduce world poverty and hunger and increase food production However, the impact of GMOs on the human health is a growing concern worldwide. Due to increased global production of GMOs, the presence of these in food products need to be monitored and has been attended by many researchers recently. The quick, simple, accurate and sensitive detection methods for these products have attracted the attention. Electrochemical DNA biosensors as one of these fast methods are highly studied by many researchers due to their high sensitivity, low cost, rapid reaction and the possibility of working in aqueous solutions. The present review will focus on several studies on the detection of GMO based on electrochemical biosensors.


1. Liao W-C, Chuang M-C, Ho J-aA. Electrochemical sensor for multiplex screening of genetically modified DNA: Identification of biotech crops by logic-based biomolecular analysis. Biosensors and Bioelectronics. 2013;50:414-20.
2. Manzanares-Palenzuela CL, Martín-Fernández B, López MS-P, López-Ruiz B. Electrochemical genosensors as innovative tools for detection of genetically modified organisms. TrAC Trends in Analytical Chemistry. 2015;66:19-31.
3. Tam PD. Genetically modified organism (GMO) detection by biosensor based on SWCNT material. Current Applied Physics. 2015;15(3):397-401.
4. James C. Executive summary. Global Status of Commercialized Biotech/GM Crops ISAAA Brief. 2011;43:20.
5.Phillips PW, McNeill H. A survey of national labeling policies for GM foods. 2000.
6. Aghili Z, Nasirizadeh N, Divsalar A, Shoeibi S, Yaghmaei P. A nanobiosensor composed of exfoliated graphene oxide and gold nano-urchins, for detection of GMO products. Biosensors and Bioelectronics. 2017;95:72-80.
7. Datukishvili N, Kutateladze T, Gabriadze I, Bitskinashvili K, Vishnepolsky B. New multiplex PCR methods for rapid screening of genetically modified organisms in foods. Frontiers in microbiology. 2015;6:757.
8. Noguchi A, Akiyama H, Nakamura K, Sakata K, Minegishi Y, Mano J, et al. A novel trait-specific real-time PCR method enables quantification of genetically modified (GM) maize content in ground grain samples containing stacked GM maize. European Food Research and Technology. 2015;240(2):413-22.
9. Fu W, Zhu P, Wang C, Huang K, Du Z, Tian W, et al. A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment. Scientific reports. 2015;5:12715.
10.Pauwels K, De Keersmaecker SC, De Schrijver A, du Jardin P, Roosens NH, Herman P. Next-generation sequencing as a tool for the molecular characterisation and risk assessment of genetically modified plants: added value or not? Trends in Food Science & Technology. 2015;45(2):319-26.
11. Liu G, Su W, Xu Q, Long M, Zhou J, Song S. Liquid-phase hybridization based PCR-ELISA for detection of genetically modified organisms in food. Food Control. 2004;15(4):303-6.
12. Feriotto G, Borgatti M, Mischiati C, Bianchi N, Gambari R. Biosensor technology and surface plasmon resonance for real-time detection of genetically modified roundup ready soybean gene sequences. Journal of Agricultural and Food Chemistry. 2002;50(5):955-62.
13. Lien TTN, Dai Lam T, An VTH, Hoang TV, Quang DT, Khieu DQ, et al. Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS. Talanta. 2010;80(3):1164-9.
14.Huang X, Zhai C, You Q, Chen H. Potential of cross-priming amplification and DNA-based lateral-flow strip biosensor for rapid on-site GMO screening. Analytical and bioanalytical chemistry. 2014;406(17):4243-9.
15. Mao-Qing W, Xiao-Yan D, Qian S, JIANG X-C. DNA biosensor prepared by electrodeposited Pt-nanoparticles for the detection of specific deoxyribonucleic acid sequence in genetically modified soybean. Chinese Journal of Analytical Chemistry. 2008;36(7):890-4.
16. Manzanares-Palenzuela CL, Martín-Clemente J, Lobo-Castañón MJ, López-Ruiz B. Electrochemical detection of magnetically-entrapped DNA sequences from complex samples by multiplexed enzymatic labelling: Application to a transgenic food/feed quantitative survey. Talanta. 2017;164:261-7.
17.Xu M, Wang R, Li Y. Electrochemical biosensors for rapid detection of Escherichia coli O157: H7. Talanta. 2017;162:511-22.
18. Arugula MA, Zhang Y, Simonian AL. Biosensors as 21st century technology for detecting genetically modified organisms in food and feed. Analytical Chemistry. 2013;86(1):119-29.
19. Sun W, Zhong J, Zhang B, Jiao K. Application of cadmium sulfide nanoparticles as oligonucleotide labels for the electrochemical detection of NOS terminator gene sequences. Analytical and bioanalytical chemistry. 2007;389(7-8):2179-84.
20. Sun W, Zhong J, Qin P, Jiao K. Electrochemical biosensor for the detection of cauliflower mosaic virus 35 S gene sequences using lead sulfide nanoparticles as oligonucleotide labels. Analytical biochemistry. 2008;377(2):115-9.
21. Ahmed MU, Saito M, Hossain MM, Rao SR, Furui S, Hino A, et al. Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification. Analyst. 2009;134(5):966-72.
22. Berti F, Lozzi L, Palchetti I, Santucci S, Marrazza G. Aligned carbon nanotube thin films for DNA electrochemical sensing. Electrochimica Acta. 2009;54(22):5035-41.
23.Sun W, Zhang Y, Hu A, Lu Y, Shi F, Lei B, et al. Electrochemical DNA biosensor based on partially reduced graphene oxide modified carbon ionic liquid electrode for the detection of transgenic soybean A2704‐12 gene sequence. Electroanalysis. 2013;25(6):1417-
24. Manzanares-Palenzuela CL, de-los-Santos-Álvarez N, Lobo-Castañón MJ, López-Ruiz B. Multiplex electrochemical DNA platform for femtomolar-level quantification of genetically modified soybean. Biosensors and Bioelectronics. 2015;68:259-65.