Effects of High Intensity Interval Training and Intermittent Fasting on VEGF Gene Expression in the Cardiac Muscle Tissue of Obese Male Rats

Document Type : Research Paper


Applied Exercise Physiology, AllamehTabataba'i University, Tehran, Iran.


Introduction: The present study aim to investigate the combined effect of high intensity interval training (HIIT) and intermittent fasting (IF) on the expression vascular endothelial grow factor (VEGF) gene in the cardiac muscle tissue of obese male rats. Methods: This study was conducted on 22 male Wistar rats that were kept on high fat -diet for 12 weeks. Afterwards the animals were randomly divided into four groups, including control, HIIT (three session for six week, 80 - 95% MRT), IF (three days for six week, 12h ours) and HIIT with IF (both protocols for six week).  Real-time polymerase chain reaction (RT- PCR) was used to measure VEGF gene expression.  Data analysis was performed using one –way analysis of variance ANOVA least significance test (p<0.05). Results:  no significant difference was observed between the groups VEGF expression (p>0.52). Conclusion: According to the results HIIT with IF and HIIT or IF alone had no significant effects on not significant on VEGF expression in the cardiac muscle tissue of the obese male rats. 


  1. Zeng J, Peng L, Zhao Q, Chen QG. Effects over 12 weeks of different types and durations of exercise intervention on body composition of young women with obesity. Science & Sports. 2021; 36(1):45-52.
  2. Prior BM, Yang HT, Terjung RL. What makes vessels grow with exercise training?. J Appl Physiol. 2004; 97(3):1119-28.
  3. Sahoo SS, Lombard JM, Ius Y, O'Sullivan R, Wood LG, Nahar P, Jaaback K, Tanwar PS. Adipose-Derived VEGF–mTOR Signaling Promotes Endometrial Hyperplasia and Cancer: Implications for Obese WomenAdipose Signaling, Obesity, and Endometrial Cancer. Mol Cancer Res. 2018; 16(2):309-21.
  4. dos Santos Vieira EF, Aidar FJ, de Matos DG, da Rosa Santos L, da Silva Junior WM, dos Santos Estevam C, dos Santos JL, de Araújo SS, Marçal AC. The Effects of High-Intensity Interval Training on VO 2 max in Different Groups: A Review. J Prof Exerc Physiol. 2019; 16(3).
  5. Ramos JS, Dalleck LC, Tjonna AE, Beetham KS, Coombes JS. The impact of high-intensity interval training versus moderate-intensity continuous training on vascular function: a systematic review and meta-analysis. J Sports Med. 2015; 45(5):679-92.
  6. Türk , W. Theel, M. J. Kasteleyn, F. M. E. Franssen, P. S. Hiemstra, A. Rudolphus, C. Taube et al. High intensity training in obesity: a Meta-analysis. J Obesity Science & Practice. 2017;3(3):258-71.
  7. Soori R, Amini AA, Choobineh S, Eskandari A, Behjat A, Ghram A, Voltarelli FA. Exercise attenuates myocardial fibrosis and increases angiogenesis-related molecules in the myocardium of aged rats. Arch Physiol Biochem. 2022; 128(1):1-6.
  8. Sara Karbalaeifar, Abbas Ali Gaeini, Mohamad Reza Kordi. The Effect of 6_ week high Intensity Interval Tarining on VEGF/COL_18 ratio and some echocardiographi indices in rats with myocardial infarction. J Kermansh Univ Med Sic. 2016; 20(3): 94-8. [In Persian].
  9. Mona, Mohamed Tah, MarwaAbd El –Rahman Mohamad, MarwaEsmaelHasanin. Effect high intensity interval training on endothellal functional in postmenopausal hypertensive patients: randomized controlled trial. Int J Physiother. 2016; 1(3):39-44.
  10. Kyoung-Han Kim, Yun Hye Kim, Joe Eun Son, Ju Hee Lee, Sarah Kim , Min Seon Choe et al. Intermittent fasting promotes adipose thermogenesis and metabolic homeostasis via VEGF – mediated alternative activation of macrophage. J Cell Research. 2017; 201727:1309-26.
  11. Robin A. Wilson, William Deasy, Christos G. Stathis, Alan Hayes, Matthew B. Cooke MB. Intermittent Fasting with or without Exercise Prevents Weight Gain and Improves Lipids in Diet – Induced obese Mice. J Nutrients. 2018; 10(3), 364.
  12. Fraisl P, Mazzone M, Schmidt T, Carmeliet P. Regulation of angiogenesis by oxygen and metabolism. J Developmental cell. 2009; 16(2):167-79.
  13. Emily RobinsonCody DurrerSvetlana SimtchoukMary E. JungJessica E. BourneElizabeth Voth, et al. Short-term high intensity and moderate – internsity continuous training reduce leukocyte TLR4 in inactive adulte at elevated risk of type 2 diabetas, J Appl Phsiol, 2015; 119:508-16.
  14. Marcus Vinicius Machado, Aline Bomfim Vieira, Fabiana Gomes da Conceic¸ao˜, Alessandro Rodrigues Nascimento, Antonio Claudio Lucas da Nobrega and Eduardo Tibirica. Exercise training dose differentially alters muscle and heart capillary density and metabolic functions in an obese with metabolic syndrome. J Exp Physiol. 2017; 102 (12):1716-28.
  15. TitisNurmasitoh, ShindyYudhaUtami, EndahKusumawardani, Abdulhalim Ahmad Najmuddin, and IkaFidianingsih. Intermittent fasting decreases oxidative stress parametersinWistar rats (Rattusnorvegicus). Universa Medicina. 2018; 37 (1):31-8.
  16. Bayati M, Gharakhanlou R, Farzad B. Adaptations of physiological performance following high-intensity interval training. J Sport Physiology. 2015; 7(26):15-32. (In Persian)
  17. Yazdanian, M. R. Asad, M. Rahimi. The effect of high intensity interval training and moderate-intensity continuous training on PGC1α and VEGF in heart muscle of male wistar rats. J Sport Physiology. 2018;10(38):111-24.
  18. Jessica Chinsomboon, Jorge Ruas, Rana K. Gupta, Robyn Thom, Jonathan Shoag, Glenn C. Rowe, Naoki Sawada, Srilatha Raghuram, and Zoltan Arany. The transcriptional coactivator PGC-1α mediates exercise-induced angiogenesis in skeletal muscle. P Natl Acad Sci USA. 2009; 106(50):21401-6.
  19. Zoltan Arany, Shi-Yin Foo, Yanhong Ma , Jorge L. Ruas, Archana Bommi-Reddy, Geoffrey Girnun, et al. HIF independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1α. J Nature. 2008; 451(7181):1008-12.
  20. Shabani M, Choobine S, Kordi MR. The Effect 8 week High intensity interval training on gene expression VEGF and PGC-1α in cardiac muscle tissue health rats. Journal science biology. 1395; 8(2):169-76. [In Persian].
  21. Bellafiore M, Battaglia G, Bianco A, Palma A. Expression pattern of angiogenic factors in healthy heart in response to physical exercise intensity. J 2019; 10:238.
  22. Morland C, Andersson KA, Haugen ØP, Hadzic A, Kleppa L, Gille A, Rinholm JE, Palibrk V, Diget EH, Kennedy LH, Stølen T. Exercise induces cerebral VEGF and angiogenesis via the lactate receptor HCAR1. J Nature communications. 2017;8(1):1-9.


  • Receive Date: 17 December 2021
  • Revise Date: 24 January 2022
  • Accept Date: 31 January 2022
  • First Publish Date: 31 January 2022