Acute Response of Oxidative Stress and Muscle Damage Biomarkers to a Single Bout of Combined Training (Resistance-Aerobic) with Different Intensities Following Q10 Supplementation

Document Type : Research Paper


1 Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran.

2 Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran.


Introduction: Nutritional supplements and controlling exercise intensity are essential to increasing muscle mass. However, the effects of such manipulations on the health of athletes may not be apparent. The present study aimed to compare the effects of combined training (resistance-aerobic) with different intensities and the short-term supplementation of coenzyme Q10 on oxidative stress and muscle damage biomarkers. Methods: This quasi-experimental study was conducted on 45 male bodybuilders who were divided into three groups of low-, moderate-, and high-intensity combined training (15 per each). Research stages included initial blood sampling, 14 days of Q10 supplementation (400 mg/day), a second blood sampling, a single bout of combined physical activity, and the final blood sampling. In each group, resistance training was initially performed with different intensities (55%, 70%, and 85% 1-RM), followed by aerobic training to consume 300 kilocalories at speeds of 8, 9.6, and 11.2 km/h. Results: Q10 supplementation had no significant effect on the baseline ​​levels of malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH), and creatine kinase (CK) (P≥0.05). After performing low-, moderate-, and high-intensity combined activities, a significant increase was observed in the levels of MDA, SOD, LDH, and CK (P≤0.05). Conclusion: According to the results, oxidative stress and muscle damage biomarkers increased in response to training intensity, while the increase was not significant at different intensities and fixed times. The performance of the participants may have influenced the obtained results. Given the lower aerobic capacity of male bodybuilders, exercise volume is the primary factor to increase exercise pressure. Supplementation may also be effective over long periods.


  1. Libardi CA, De Souza GV, Cavaglieri CR, Madruga VA, Chacon-Mikahil MP. Effect of resistance, endurance, and concurrent training on TNF-alpha, IL-6, and CRP. Med Sci Sports Exerc. 2012;44(1):50-6.
  2. Schroeder EC, Franke WD, Sharp RL, Lee DC. Comparative effectiveness of aerobic, resistance, and combined training on cardiovascular disease risk factors: A randomized controlled trial. PLoS One. 2019;14(1):e0210292.
  3. Bouzid MA, Hammouda O, Matran R, Robin S, Fabre C. Changes in oxidative stress markers and biological markers of muscle injury with aging at rest and in response to an exhaustive exercise. PLoS One. 2014;9(3):e90420.
  4. Bloomer RJ, Canale RE, McCarthy CG, Farney TM. Impact of oral ubiquinol on blood oxidative stress and exercise performance. Oxid Med Cell Longev. 2012;2012:465020.
  5. Deminice R, Trindade CS, Degiovanni GC, Garlip MR, Portari GV, Teixeira M, et al. Oxidative stress biomarkers response to high intensity interval training and relation to performance in competitive swimmers. J Sports Med Phys Fitness. 2010;50(3):356-62.
  6. Wilson JM, Lowery RP, Joy JM, Loenneke JP, Naimo MA. Practical blood flow restriction training increases acute determinants of hypertrophy without increasing indices of muscle damage. J Strength Cond Res. 2013;27(11):3068-75.
  7. Abedi B, Fatolahi H, Kouhidehkordi S, Zolfaghari GA. The Effects of Copenhagen Football Test on Glutathione Reductase and Catalase Activity in Female Football Players. Asian J Sports Med. 2017;8(1):e41473.
  8. Dekany M, Nemeskeri V, Gyore I, Harbula I, Malomsoki J, Pucsok J. Antioxidant status of interval-trained athletes in various sports. Int J Sports Med. 2006;27(2):112-6.
  9. Nejatmand N, Ramezani A, Barati AH. Effect of Consumption short-term CoQ10 supplementation on markers of delayed onset muscle soreness. Razi Journal of Medical Sciences. 2014;21(119):77-85.
  10. Nejatmand N, Ramezani A, Barati AH. Compare the effect of Consumption CoQ10 Supplement on aerobic power, anaerobic and Muscle soreness in athletes and non athletes. Journal of Shahrekord University of Medical Sciences. 2016;18(1):93-104.
  11. Crane FL. Biochemical functions of coenzyme Q10. J Am Coll Nutr. 2001;20(6):591-8.
  12. Kon M, Kimura F, Akimoto T, Tanabe K, Murase Y, Ikemune S, et al. Effect of Coenzyme Q10 supplementation on exercise-induced muscular injury of rats. Exerc Immunol Rev. 2007;13:76-88.
  13. Kon M, Tanabe K, Akimoto T, Kimura F, Tanimura Y, Shimizu K, et al. Reducing exercise-induced muscular injury in kendo athletes with supplementation of coenzyme Q10. Br J Nutr. 2008;100(4):903-9.
  14. Cooke M, Iosia M, Buford T, Shelmadine B, Hudson G, Kerksick C, et al. Effects of acute and 14-day coenzyme Q10 supplementation on exercise performance in both trained and untrained individuals. J Int Soc Sports Nutr. 2008;5:8.
  15. Mayhew JL, Prinster JL, Ware JS, Zimmer DL, Arabas JR, Bemben MG. Muscular endurance repetitions to predict bench press strength in men of different training levels. J Sports Med Phys Fitness. 1995;35(2):108-13.
  16. Hidaka T, Fujii K, Funahashi I, Fukutomi N, Hosoe K. Safety assessment of coenzyme Q10 (CoQ10). Biofactors. 2008;32(1-4):199-208.
  17. Yu JG, Carlsson L, Thornell LE. Evidence for myofibril remodeling as opposed to myofibril damage in human muscles with DOMS: an ultrastructural and immunoelectron microscopic study. Histochem Cell Biol. 2004;121(3):219-27.
  18. Wang XL, Rainwater DL, Mahaney MC, Stocker R. Cosupplementation with vitamin E and coenzyme Q10 reduces circulating markers of inflammation in baboons. Am J Clin Nutr. 2004;80(3):649-55.
  19. Bogdanis GC, Stavrinou P, Fatouros IG, Philippou A, Chatzinikolaou A, Draganidis D, et al. Short-term high-intensity interval exercise training attenuates oxidative stress responses and improves antioxidant status in healthy humans. Food Chem Toxicol. 2013;61:171-7.
  20. Radak Z, Chung HY, Goto S. Systemic adaptation to oxidative challenge induced by regular exercise. Free Radic Biol Med. 2008;44(2):153-9.
  21. Gaeini A, Vatani D, Ashrafi J, Mogharnasi M. The Short–Term and Long-Term Effects of Sprint, Endurance and Concurrent Exercise Training on Plasmatic Lactate Dehydrogenase, Creatine Kinase, and Malondialdehyde in Rats. Journal of Sport Biosciences. 2011;3(8).
  22. Sumida S, Doi T, Sakurai M, Yoshioka Y, Okamura K. Effect of a single bout of exercise and beta-carotene supplementation on the urinary excretion of 8-hydroxy-deoxyguanosine in humans. Free Radic Res. 1997;27(6):607-18.
  23. Milias GA, Nomikos T, Fragopoulou E, Athanasopoulos S, Antonopoulou S. Effects of eccentric exercise-induced muscle injury on blood levels of platelet activating factor (PAF) and other inflammatory markers. Eur J Appl Physiol. 2005;95(5-6):504-13.
  24. Modi K, Santani DD, Goyal RK, Bhatt PA. Effect of coenzyme Q10 on catalase activity and other antioxidant parameters in streptozotocin-induced diabetic rats. Biol Trace Elem Res. 2006;109(1):25-34.
  25. Zuliani U, Bonetti A, Campana M, Cerioli G, Solito F, Novarini A. The influence of ubiquinone (Co Q10) on the metabolic response to work. J Sports Med Phys Fitness. 1989;29(1):57-62.
  26. Deminice R, Rosa FT, Franco GS, Jordao AA, de Freitas EC. Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition. 2013;29(9):1127-32.
  27. Liu JF, Chang WY, Chan KH, Tsai WY, Lin CL, Hsu MC. Blood lipid peroxides and muscle damage increased following intensive resistance training of female weightlifters. Ann N Y Acad Sci. 2005;1042:255-61.
  28. Fariborz Hovanloo, Maryam Nourshahi, Ehsan Amini, Mina Sahami. Effect of short term supplementation with L-carnitin and coenzyme Q10 on aerobic and anaerobic exercise performance in sedentary college men. Pajoohande. 2012;17(1):8-17.
  29. Kaikkonen J, Kosonen L, Nyyssonen K, Porkkala-Sarataho E, Salonen R, Korpela H, et al. Effect of combined coenzyme Q10 and d-alpha-tocopheryl acetate supplementation on exercise-induced lipid peroxidation and muscular damage: a placebo-controlled double-blind study in marathon runners. Free Radic Res. 1998;29(1):85-92.