Journal of Nutrition,Fasting and Health

Journal of Nutrition,Fasting and Health

Modelling of Staphylococcus Aureus under the Effect of Carum Copticum Essential Oil, pH, Temperature, and Inoculum Level

Document Type : Research Paper

Authors
Sara Mohamadi 1
Saeid Khanzadi 1
Abdollah Jamshidi 1
Mohammad Azizzadeh 2
1 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
10.22038/jnfh.2022.63675.1377
Abstract
Staphylococcus aureus is among the major causes of foodborne outbreaks globally. To limit its potential risks and predict its growth behaviors, it is crucial to define the growth boundaries of Staphylococcus aureus. So, this experiment was designed to estimate the growth behavior of Staphylococcus aureus in brain heart infusion (BHI) broth while affected by various concentrations of Carum copticum EO (0, 0.015, 0.030, 0.045%), pH (5, 6, 7), temperature (25, 35 ˚C), and inoculum levels (103, 105 CFU ml-1). The assay was performed with 48 treatment conditions in triplicate. Visible turbidity represents growth onset was checked daily during 30 days of trial. According to the accelerated failure time (AFT) approach, a parametric survival model was chosen to predict the impact of selected variables on Staphylococcus aureus growth. GC-MS assay had quantified sixteen (16) compounds constituting 98.88% of pure oil. Based on our findings, the major components of essential oil were identified as thymol (57.18%), ρ-cymene (22.55%), γ-terpinene (13.07%), and trans-anethole (1.7%). The MIC value of the EO was 0.625 μl ml-1. The median time to detection of bacterial growth was six days. All the predictor variables showed a significant effect on time to initiate the bacterial growth (p < 0.05). The ultimate model could precisely estimate the growth responses of Staphylococcus aureus. 
Keywords
Carum copticum essential oil
Predictive modeling
Staphylococcus aureus
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Journal of Nutrition,Fasting and Health
Volume 10, Issue 2
Spring 2022
Pages 94-102

  • Receive Date 12 February 2022
  • Accept Date 13 March 2022