Short-Term Effect of Aerobic Exercise and High-Fat Diet and Consumption of Curcumin Extract on Leptin Gene Expression in Liver Tissue in Male Rats

Document Type : Research Paper

Authors

1 Department of Sports Physiology, Islamic Azad University, Damghan Branch, Damghan, Iran.

2 Department of Physical Education, Faculty of Humanities, Islamic Azad University, Damghan Branch, Damghan, Iran.

10.22038/jnfh.2022.66628.1396

Abstract

Introduction: Leptin is a protein hormone with a spiral structure similar to cytokines, which is mainly synthesized and released by subcutaneous fat cells in a steady pulsating manner with a peak secretion near midnight. The aim of this study was to compare the short-term effects of aerobic exercise and high-fat diet and curcumin extract on leptin gene expression in the liver tissue of male rats. Methods: Fifty male rat with two-month-old were prepared and divided into five groups: control(G1), high-fat diet(G2), curcumin and high-fat diet(G3), exercise and high-fat diet(G4), curcumin+exercise+high-fat diet(G5). Data analysis was compared using one-way analysis of variance. Bonferroni's post hoc test was used to accurately determine the differences between groups. The mean of intra-group differences was also compared using the t-test of the sample. Results: The results showed a significant difference in the average leptin gene expression of subjects among 5 groups. The results of Bonferroni's post hoc test also showed an increase in the high-fat diet group compared to the control group(P=0.0001), and a decrease in the high-fat diet + curcumin group(P=0.0001), the high-fat diet + exercise group(P=0.0001), and the high-fat diet + exercise + curcumin group compared to the control group(P=0.0001). Conclusion: Leptin gene is expressed under the influence of short-term exercises and the consumption of curcumin, which is an antioxidant. So that the use of each agent alone causes a decrease or increase in the leptin gene expression in the liver, and the simultaneous use of both factors causes a decrease in the leptin gene expression of these cells in the muscle tissue.

Keywords


  1. Babalwa & Malherbe, Christiaan & Mamushi, Mokadi & Muller, Christo & Joubert, Elizabeth & Louw, Johan & Pheiffer, Carmen. (2019). Adipose tissue as a possible therapeutic target for polyphenols: A case for Cyclopia extracts as anti-obesity nutraceuticals. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 120. 109439. 10.1016/j.biopha.2019.109439.
  2. Baek K, Kang J, Lee J, Kim M, Baek JH. (2018). The Time Point-Specific Effect of Beta-Adrenergic Blockade in Attenuating High Fat Diet-Induced Obesity and Bone Loss. Calcified tissue international. (19):1-13.
  3. Dabidi Roshan, v., & Barzegarzadeh, h. (2011). The effect of obesity and exercise intensity on serum adiponectin response and some biochemical indexes in young women. olympic, 19(2 (serial 54)), 141-154. https://www.sid.ir/en/journal/ViewPaper.aspx?id=289302
  4. Frayn K. N. (2002). Insulin resistance, impaired postprandial lipid metabolism and abdominal obesity. A deadly triad. Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 11 Suppl 2, 31–40.
  5. Frayn KN , Fielding BA , Humphreys SM , Coppack SW. (1996). Nutritional influences on human adipose – tissue metabolism. Biochem Soc Trans; 24(2) : 422-6.
  6. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y,et al. (2017). Increased oxidative stress in obesity and its impact on metabolic syndrome. The Journal of clinical investigation.114(12):1752-61.
  7. Jin, B.U. (2018). Polyphenol-enriched fractions of Cyclopia intermedia selectively affect lipogenesis and lipolysis in 3T3-L1 adipocytes, Planta Med. 84.100–110.
  8. Kandula V, Kosuru R, Li H, Yan D, Zhu Q, Lian Q, et al. (2016). Forkhead box transcription factor 1: role in the pathogenesis of diabetic cardiomyopathy. Cardiovascular diabetology.15(1):44.
  9. Larijani B, Ghodsi M. (2005). LEPTIN: A NEW ADYPOCYTE HORMONE AND ITS ROLE IN THE OBESITY. ijdld. 4 (3) :1-10
  10. Martins R, Lithgow GJ, Link W. (2016). Long live FOXO: unraveling the role of FOXO proteins in aging and longevity. Aging cell.15(2):196-207.
  11. Milan G, Romanello V, Pescatore F, Armani A, Paik JH, Frasson L, et al. (2015). Regulation of autophagy and the ubiquitin–proteasome system by the FoxO transcriptional network during muscle atrophy. Nature communications.10;6:6670.
  12. Mirghani, S. J., Peeri, M., Yaghoobpour Yekani, O., Zamani, M., Feizolahi, F., Nikbin, S., Derakhshideh, A., Mousavi, N., Khojasteh, Z., Nasrollahi, Z., Khorasani, E., Ghodousi Johari, E., Afshar, T., & Azarbayjani, M. A. (2019). Role or Synergistic Interaction of Adenosine and Vitamin D3 Alongside High-Intensity Interval Training and Isocaloric Moderate Intensity Training on Metabolic Parameters: Protocol for an Experimental Study. JMIR research protocols, 8(1), e10753. https://doi.org/10.2196/10753
  13. Park, H. S., Park, J. Y., & Yu, R. (2005). Relationship of obesity and visceral adiposity with serum concentrations of CRP, TNF-alpha and IL-6. Diabetes research and clinical practice, 69(1), 29–35. https://doi.org/10.1016/j.diabres.2004.11.007
  14. Perl A. (2015). mTOR activation is a biomarker and a central pathway to autoimmune disorders, cancer, obesity, and aging. Annals of the New York Academy of Sciences. 1346(1):33-44.
  15. Raoufi A, sirous F, Hoseini A. (2019). The Effect of Eight Weeks of Curcumin Supplementation on the Expression of Some Regulatory Genes of Atrophic Processes in the Heart Tissue of Fatty Adult Fatty Rats. J Fasa Univ Med Sci.9 (2) :1425-1432.
  16. Relling DP, Esberg LB, Fang CX, Johnson WT, Murphy EJ, Carlson EC et al. (2006). High-fat diet-induced juvenile obesity leads to cardiomyocyte dysfunction and upregulation of Foxo3a transcription factor independent of lipotoxicity and apoptosis. Journal of hypertension. 12(3):549-61.
  17. Singh, S. P., Bellner, L., Vanella, L., Cao, J., Falck, J. R., Kappas, A., & Abraham, N. G. (2016). Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice. Journal of nutrition and metabolism, 2016, 9039754. https://doi.org/10.1155/2016/9039754
  18. Sirico, Felice & Bianco, Antonio & D'Alicandro, Giovanni & Castaldo, Clotilde & Montagnani, Stefania & Spera, Rocco & Di Meglio, Franca & Nurzynska, Daria. (2020). Effects of Physical Exercise on Adiponectin, Leptin, and Inflammatory Markers in Childhood Obesity: Systematic Review and Meta-Analysis. Childhood Obesity. 14. 10.1089/chi.2017.0269
  19. Sonoli SS, Shivprasad S, Prasad CV, Patil AB, Desai PB, Somannavar MS. (2011). Visfatin--a review. Eur Rev Med Pharmacol Sci. 15(1):9-14.
Volume 10, Issue 3 - Serial Number 3
September 2022
Pages 215-221
  • Receive Date: 08 July 2022
  • Revise Date: 30 July 2022
  • Accept Date: 04 September 2022
  • First Publish Date: 05 September 2022