The Simultaneous Effect of Endurance Training and Octopamine Supplementation on Octopamine and its Receptors in the Visceral Fat Tissue of Rats Treated with Deep Fried Oil (DFO)

Document Type : Research Paper

Authors

Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

10.22038/jnfh.2022.66872.1397

Abstract

Introduction: The role of exercise and some supplements in lipolysis has been reported, but given limited information on the simultaneous effect of endurance training (ET) and octopamine (Oct), the present study aimed to investigate the interactive effect of these two interventions on adipose tissue lipolysis with emphasis on octopamine receptors in rats treated with deep fried oil (DFO). Methods: Thirty male Wistar rats (20-18 weeks old and 280-320 g) were divided into five groups, including (1) healthy control (C), (2) DFO, (3) Oct+DFO, (4) ET+DFO and (5) DFO+Oct+ET. Aerobic training was performed for four weeks, five sessions per week with an intensity of 16-26 m / min and equivalent to 50-65% VO2max; also, 81 μmol/kg octopamine supplementation was intraperitonally injected to rats 5 days a week. Two-way analysis of variance and Bonferroni's post hoc test were used to analyze the data. Results: ET increases Octβ-R expression (P=0.02) and Oct protein concentration (P=0.001) in the visceral adipose tissue of rats exposed to DFO. Oct supplementation increases Octα-R (P=0.01) expression in the visceral adipose tissue of rats exposed to DFO. Also, ET and Oct do not have a synergistic effect on Octβ-R (P=0.91), Octα-R (P=0.65) and Oct protein concentration (P=0.16) in the visceral adipose tissue of rats exposed to DFO. Conclusion: It seems that although training and octopamine supplementation alone play a role in increasing the protein concentration of octopamine and its receptors, these two interventions do not have a synergistic effect on lipolysis by emphasizing the octopamine receptor pathway.

Keywords


  1. Tadesse Zula A, Fikre Teferra T. Effect of frying oil stability over repeated reuse cycles on the quality and safety of deep-fried Nile tilapia fish (Oreochromis niloticus): a response surface modeling approach. Int J Food Prop. 2022;25(1):315–25.

    1. Masbah N, Nordin SH, Jaarin K. The Role of Antioxidants in Attenuating Heated Oil-Induced Cardiovascular Effects: A Review. Biomed Pharmacol J. 2017;10(3):1037–53.
    2. Solís-Guevara FD, Ruiz Mamani PG, Saintila J. Dietary Regimen, Overweight, and Obesity in Human Nutrition Students and Other Majors: A Cross-Sectional Study. J Nutr Metab. 2022;2022.
    3. Fontana E, Morin N, Prévot D, Carpéné C. Effects of octopamine on lipolysis, glucose transport and amine oxidation in mammalian fat cells. Comp Biochem Physiol Part C Pharmacol Toxicol Endocrinol. 2000;125(1):33–44.
    4. Sujkowski A, Gretzinger A, Soave N, Todi S V, Wessells R. Alpha-and beta-adrenergic octopamine receptors in muscle and heart are required for Drosophila exercise adaptations. PLoS Genet. 2020;16(6):e1008778.
    5. Kaya-Zeeb S, Engelmayer L, Straßburger M, Bayer J, Bähre H, Seifert R, et al. Octopamine drives honeybee thermogenesis. Elife. 2022;11:e74334.
    6. Vesali M, Azarbayjani MA, Peeri M. Effect of Aerobic Training and Octopamine Supplementation on the Expression of Octopamine Receptors in the Visceral Adipose Tissue of Rats Exposed to Deep Fried Oils. Gene, Cell Tissue. 2021;8(4).
    7. Dezhan M, AZARBAYJANI MALI, PEERI M. Effect of aerobic and octopamine supplementation on the expression of ACC and ACYL genes and HDL/LDL ratio in visceral visceral adipose tissue of DFO recipient. 2020;
    8. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Arch Physiol Biochem. 2020;1–6.
    9. Engin B, Willis SA, Malaikah S, Sargeant JA, Yates T, Gray LJ, et al. The effect of exercise training on adipose tissue insulin sensitivity: A systematic review and meta‐analysis. Obes Rev. 2022;e13445.
    10. Kianmehr P, Azarbayjani MA, Peeri M, Farzanegi P. Synergic effects of exercise training and octopamine on peroxisome proliferator-activated receptor-gamma coactivator-1a and uncoupling protein 1 mRNA in heart tissue of rat treated with deep frying oil. Biochem Biophys reports. 2020;22:100735.
    11. Azarbayjani MA, Peeri M, Farzanegi P. The effects of aerobic exercise training with octopamine supplementation on cardiomyocyte apoptosis induced by deep-frying oil: The role of caspase and procaspase 3. Clin Nutr ESPEN. 2022;
    12. Abdollahi S, Mohamadzadeh SK, Azizbeigi K, Etemad Z. The Effect of aerobic training and octopamine on HSP70 and Caspase-3 protein expression in brown adipose tissue in rats received deeply heated oil treatment. 2020;
    13. Shokrzadeh M, Keshavarz-Maleki R, Haghi-Aminjan H, Masoumi S, Yazdani-Charati J. Subacute and subchronic toxicity effects of the Stachys lavandulifolia water extract in rat. J Maz Univ Med Sci. 2014;23(108):69–75.
    14. Talaei SA, Mohammadifar M, Azami A, Salami M. Effect of Interaction of Age and Changing in Visual Experience During Critical Period of Brain Development on Expression of Melatonin Receptors in Rat’s Hippocampus. Horiz Med Sci. 2015;21(3):163–8.
    15. Shokri F, Azarbayjani MA, Peeri M, Ghazalian F. The Effect of Octopamine and Aerobic Exerciseand on Genes Affecting Angiogenesis of Visceral Adipose Tissue in Rats Fed with Deep-Fried Oil. J Nutr Fasting Heal. 2020;8(3):192–8.
    16. Lu Y-F, Lo Y-C. Effect of deep frying oil given with and without dietary cholesterol on lipid metabolism in rats. Nutr Res. 1995;15(12):1783–92.
    17. Sujkowski A, Ramesh D, Brockmann A, Wessells R. Octopamine drives endurance exercise adaptations in Drosophila. Cell Rep. 2017;21(7):1809–23.
    18. Hana S, Lange AB. Cloning and functional characterization of Octβ2-receptor and Tyr1-receptor in the Chagas disease vector, Rhodnius prolixus. Front Physiol. 2017;8:744.
    19. Kastner KW, Shoue DA, Estiu GL, Wolford J, Fuerst MF, Markley LD, et al. Characterization of the Anopheles gambiae octopamine receptor and discovery of potential agonists and antagonists using a combined computational-experimental approach. Malar J. 2014;13(1):1–14.
    20. Visentin V, Morin N, Fontana E, Prévot D, Boucher J, Castan I, et al. Dual action of octopamine on glucose transport into adipocytes: inhibition via β3-adrenoceptor activation and stimulation via oxidation by amine oxidases. J Pharmacol Exp Ther. 2001;299(1):96–104.
    21. Stohs SJ, Shara M, Ray SD. p‐Synephrine, ephedrine, p‐octopamine and m‐synephrine: Comparative mechanistic, physiological and pharmacological properties. Phyther Res. 2020;
    22. Beaumont RE, Cordery P, James LJ, Watson P. Supplementation with a low-dose of octopamine does not influence endurance cycling performance in recreationally active men. J Sci Med Sport. 2017;20(10):952–6.
    23. Friedlander AL, Casazza GA, Horning MA, Buddinger TF, Brooks GA. Effects of exercise intensity and training on lipid metabolism in young women. Am J Physiol Metab. 1998;275(5):E853–63.
    24. Ghafari M, Faramarzi M, Banitalebi E. Compar two different endurance training intensities on perilipin 3 protein expression in skeletal muscle, serum glucose levels and insulin in streptozotocin-induced diabetic rats. Iran J Diabetes Metab. 2018;17(4):198–205.
  • Receive Date: 21 July 2022
  • Revise Date: 09 September 2022
  • Accept Date: 12 September 2022
  • First Publish Date: 12 September 2022