The Effect of Resistance Training on Heart Damage Risk Factors in Adult Male Rats Exposed to Nandrolone

Document Type : Research Paper


1 Department of Exercise Physiology, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.

2 Department of Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.

3 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.


Introduction: Athletes and non-athletes use nandrolone (Na) for different purposes as a derivative of testosterone, and various results have been reported regarding its use. This study aimed to evaluate the effect of a period of resistance training (RT) on SOD, MDA, CRP, and cTn-C in the cardiac tissue of Na- exposed rats. Methods: In the present experimental research, 20 male Wistar rats aged 8-10 weeks were randomly divided into four groups of five animals: (1) control, (2) sham, (3) Na (10mg/kg), (4) Na+RT. The RT group performed 1m ladder climbing in three sessions weekly for eight weeks. One-way ANOVA and Tukey’s post hoc tests were implemented to analyze the data (P≤0.05). Results: Levels of SOD were significantly lower in the Na+RT group compared to the control group (P=0.006). However, no significant difference was observed in levels of SOD in the Na group compared to the Na+RT group (P=0.99). In addition, the levels of MDA and CRP in the Na+RT group were significantly lower than in the Na group (P≤0.05). Histological studies showed that Na intoxication, RT, and RT with Na use did not affect tissue changes in the study groups. Conclusion: Based on the results, nandrolone with and without resistance training increases heart disease risk indicators. However, the risk of heart disease in training and abusing nandrolone is less than the normal condition of this steroid without training.


  1. Liu J-D, Wu Y-Q. Anabolic-androgenic steroids and cardiovascular risk. Chin Med J (Engl). 2019; 20; 132(18): 2229–36.
  2. Hernández-Guerra AI, Tapia J, Menéndez-Quintanal LM, Lucena JS. Sudden cardiac death in anabolic androgenic steroids abuse: case report and literature review. Forensic Sci Res. 2019;4(3):267–73.
  3. Tofighi A, Shirpoor M, Ansari MHK, Shirpoor A, Zerehpoosh M. The effect of nandrolone treatment with and without enforced swimming on histological and biochemical changes in the heart and coronary artery of male rats. Anatol J Cardiol. 2017; 17(3):176-83.
  4. Piacentino D, D Kotzalidis G, Del Casale A, Rosaria Aromatario M, Pomara C, Girardi P, et al. Anabolic-androgenic steroid use and psychopathology in athletes: A systematic review. Curr Neuropharmacol. 2015;13(1):101–21.
  5. Rostami A, Hosseini S A, Azarbayjani M A. The effect of two months resistance training with and without Crocin consumption on catalase and glutathione peroxidase of heart tissue of nandrolone poisoned rats. Journal Of Torbat Heydariyeh University Of Medical Sciences. 2019; 6 (4) :22-32.
  6. Pinheiro JL, Maia BP, Lima AB de, Domingues RJ de S, Oliveira FRT, Freitas JJ da S, et al. Nandrolone decanoate is prooxidant in the myocardium of exercised or sedentary rats. Rev Bras Med do Esporte. 2020;26:16–20.
  7. Grace FM, Davies B. Raised concentrations of C reactive protein in anabolic steroid using bodybuilders. Br J Sports Med. 2004;38(1):97–8.
  8. Hassan DAE, Ghaleb SS, Abdelmenem A, Nabil S, Alim MAA. The toxic effects of anabolic steroids “nandrolone decanoate” on cardiac and skeletal muscles with the potential ameliorative effects of silymarin and fenugreek seeds extract in adult male albino rats. BMC Pharmacol Toxicol. 2023;24(1):1–10.
  9. Jevđević M, Jovanović M, Jeremić N, Canković M, Jeremić J, Živković V, et al. Acute effects of nandrolone decanoate on oxidative stress in isolated rat heart. Arch Biol Sci. 2015;67(1):331–7.
  10. Heidari E, Hosseini SA, Azarbayjani MA. Effect of nandrolone treatment with and without resistance training on superoxide dismutase concentration and pathology of kidney tissue in rats. J Clin Res Paramed Sci. 2020; 9(2):e102497.
  11. Chaves EA, Fortunato RS, Carvalho DP, Nascimento JHM, Oliveira MF. Exercise-induced cardioprotection is impaired by anabolic steroid treatment through a redox-dependent mechanism. J Steroid Biochem Mol Biol. 2013;138:267–72.
  12. Rahgozar A, Ghasemi Hamidabadi H, Shokri S, Moayeri A, Esmaeilnejhad Moghaddam A. Effect of human chorionic gonadotropin on the destructive effects of nandrolone decanoate on sperm parameters of testes in adult rats. Armaghane Danesh. 2015;20(9):811–24.
  13. Shokrzadeh M, Azadbakht M, Shakibamanesh H. The hepatoprotective effect of Arnebia euchroma hydro-alcoholic extract against liver toxicity induced by CCl4 in mice. Pharm Biomed Res. 2017;3(4):23–9.
  14. Tahtamouni LH, Mustafa NH, Hassan IM, Ahmad IM, Yasina SR, Abdallaa MY. Nandrolone decanoate administration to male rats induces oxidative stress, seminiferous tubules abnormalities, and sperm DNA fragmentation. Jordan J Biol Sci. 2010;3(4):165–74.
  15. Pergolizzi B, Carriero V, Abbadessa G, Penna C, Berchialla P, De Francia S, et al. Subchronic nandrolone administration reduces cardiac oxidative markers during restraint stress by modulating protein expression patterns. Mol Cell Biochem. 2017;434(1):51–60.
  16. Yin R, Tian F, Frankenberger B, de Angelis MH, Stoeger T. Selection and evaluation of stable housekeeping genes for gene expression normalization in carbon nanoparticle-induced acute pulmonary inflammation in mice. Biochem Biophys Res Commun. 2010;399(4):531–6.
  17. Joukar S, Fathpour H, Kordestani Z. Nandrolone plus moderate exercise increases the susceptibility to lethal arrhythmias. Res Cardiovasc Med. 2015;4(2): 26233.
  18. Sretenovic J, Zivkovic V, Srejovic I, Milosavljevic Z. The effects of high doses of nandrolone decanoate on cardiac muscle tissue. Serbian J Exp Clin Res. 2016; 17(4): 303-8
  19. Tanno AP, Rosa KT, Cunha TS, Giordano FCL, Calil CM, Guzzoni V, et al. Nandrolone and resistance training induce heart remodeling: role of fetal genes and implications for cardiac pathophysiology. Life Sci. 2011;89(17–18):631–7.
  20. Sadowska-Krępa E, Kłapcińska B, Jagsz S, Sobczak A, Chrapusta SJ, Chalimoniuk M, et al. High-dose testosterone propionate treatment reverses the effects of endurance training on myocardial antioxidant defenses in adolescent male rats. Cardiovasc Toxicol. 2011;11(2):118–27.
  21. Derakhshandeh M, Taghian F, Dehkordi KJ, Hosseini SA. Synchronized resistance training and bioactive herbal compounds of Tribulus Terrestris reverse the disruptive influence of Stanozolol. Steroids. 2022;182:109000.
  22. Agahi MRH, Mosallanejad Z, Salehi OR. The effects of resistance training and spirulina on the performance of the antioxidant system with emphasis on mir125b, mir146a and cognitive function in stanazolol-induced neurotoxicity in rats. Chem Biol Interact. 2022;110112.
  23. Frankenfeld SP, Oliveira LP, Ortenzi VH, Rego-Monteiro ICC, Chaves EA, Ferreira AC, et al. The anabolic androgenic steroid nandrolone decanoate disrupts redox homeostasis in liver, heart and kidney of male Wistar rats. PLoS One. 2014;9(9):e102699.