The Simultaneous Effect of Curcumin Extract Supplementation and Aerobic Exercise on Leptin and Adiponectin Gene Expression in Visceral Adipose Tissue of Rats Receiving A High-Fat Diet

Document Type : Research Paper


1 PhD Student, Department of Sports Physiology, Islamic Azad University, Damghan Branch, Damghan, Iran.

2 Assistance Professor, Department of Physical Education, Faculty of Humanities, Islamic Azad University, Damghan Branch, Damghan, Iran.

3 Associate Professor, Department of Physical Education, Faculty of Humanities, Islamic Azad University, Damghan Branch, Damghan, Iran.


Introduction: Herbal remedies can be helpful in the prevention and treatment of obesity, overweight, and related disorders. However, how aerobic exercise and curcumin supplementation interact with adipose tissue is still unclear. This study aimed to determine how aerobic exercise and supplementation with curcumin extract would affect leptin and adiponectin gene expression in visceral adipose tissue of rats fed a high-fat diet. Methods: In this study, 50 male rats were divided into five groups of equal size: control, high-fat diet, curcumin and high-fat diet, aerobic exercise and high-fat diet, and aerobic exercise and curcumin and high-fat diet. Five sessions per week of aerobic exercise were performed for six weeks at a speed of 25 to 29m/min for 20 to 45 minutes. Rats were given high-fat food emulsion of 0.5mg/kg of body weight per day for six weeks to simulate high-fat food groups. One-way analysis of variance (ANOVA) and Tukey’s post hoc test were employed in SPSS software version 22 to analyze the results. Results: There was no difference between the short-term effects of aerobic exercise, a high-fat diet, and curcumin extract on adiponectin gene expression in male rats (P=0.05). The short-term effects of aerobic exercise, a high-fat diet, and curcumin extract on the leptin gene expression in adipose tissue in male rats were comparable and not significantly different. Consuming curcumin, an antioxidant, and brief exercise affected the fat gene expression. Conclusions: Based on the results, both agent alone decreases or increases the expression of the adiponectin and leptin genes in fat, and when both agents are consumed simultaneously, the expression increases.


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