Effect of Orange Peel Essential Oil as a Natural Preservative on Characteristics of Turkey Meat Stored in Refrigerator

Document Type : Research Paper


1 Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

2 MSc in Food Hygiene and Quality control, Amol University of Special Modern Technologies, Amol, Iran


Introduction: The tendency to consume turkey meat and meat products is increasing due to its high nutritional value. But due to its perishability, many researchers are looking for new solutions to increase its storage time and maintain its quality. Thus the purpose of this study was to evaluate the chemical composition of orange peel essential oil (OPEO) and its effect on the microbial, physicochemical and sensory properties of turkey meat during 12 days of storage at refrigerator temperature. Methods: The chemical composition of OPEO was identified using GC/MS device. Three groups of turkey meat samples (control, 0.5 and 1%) of OPEO were packed and kept in the refrigerator and at regular intervals (days 0, 3, 6, 9, 12) for microbial tests (total count of aerobic, Pcychrotrophic, Enterobacteriaceae, Pseudomonas aeruginosa, lactic acid, MIC and MBC), chemical (pH, TV-N, TBARS) and sensory (taste, aroma, appearance, texture and overall acceptance) were evaluated. Results: The results of GC/MS showed the presence of effective compounds with antimicrobial and antioxidant activity, especially D-limonene (71.47%). The results of microbial tests showed that treatments of turkey meat containing 1% OPEO had a significant effect (P<0.05) on the reduction of the bacteria population compared to the treatment of 0.5% OPEO and control samples. The MIC for Listeria monocytogenes and Pseudomonas aeruginosa was determined as 4 mg/ml and MBC was determined as 8 and 4 mg/ml, respectively. Lower values ​​of pH, TV-N and TBARS, the highest sensory scores in terms of taste, aroma, appearance, texture and general acceptability were obtained in turkey meat treatments containing orange peel essence compared to the control group. Conclusions: It can be said that due to its antimicrobial properties OPEO can be used as a natural preservative to increase the shelf life and sensory improvement of turkey meat samples during storage at refrigerator temperature.


Main Subjects

  1. Rahman SM, Ding T, Oh DH. Effectiveness of low concentration electrolyzed water to inactivate foodborne pathogens under different environmental conditions. International journal of food microbiology. 2010;139(3):147-53.
  2. M, Ilkhani. F (). The principles of turkey breeding. First edition, Academic Jihad of Urmia branch. 2010:7.
  3. Kazemeini H, Azizian A, Adib H. Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion. International journal of food microbiology. 2021;344:109104.
  4. Casaburi A, Piombino P, Nychas GJ, Villani F, Ercolini D. Bacterial populations and the volatilome associated to meat spoilage. Food Microbiology. 2015;45:83-102.
  5. Salahi A. A review of the turkey meat production industry in Iran. Zootec International. 2014;36:24-9.
  6. Petrou S, Tsiraki M, Giatrakou V, Savvaidis IN. Chitosan dipping or oregano oil treatments, singly or combined on modified atmosphere packaged chicken breast meat. International journal of food microbiology. 2012;156(3):264-71.
  7. Hugo CJ, Hugo A. Current trends in natural preservatives for fresh sausage products. Trends in Food Science & Technology. 2015;45(1):12-23.
  8. Danka S, Dionyz M, Hanna R. Effects of dietary rosemary extract and alfa tocopherol on the performance of chickens, meat quality and lipid oxidation in meat.2007.
  9. Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food chemistry. 2005;89(4):569-75.
  10. Kang HJ, Chawla SP, Jo C, Kwon JH, Byun MW. Studies on the development of functional powder from citrus peel. Bioresource technology. 2006;97(4):614-20.
  11. Maeda-Yamamoto M, Kawahara H, Tahara N, Tsuji K, Hara Y, Isemura M. Effects of tea polyphenols on the invasion and matrix metalloproteinases activities of human fibrosarcoma HT1080 cells. Journal of Agricultural and Food Chemistry. 1999;47(6):2350-4.
  12. Rafiq S, Kaul R, Sofi SA, Bashir N, Nazir F, Nayik GA. Citrus peel as a source of functional ingredient: A review. Journal of the Saudi Society of Agricultural Sciences. 2018;17(4):351-8.
  13. Allaf T, Tomao V, Besombes C, Chemat F. Thermal and mechanical intensification of essential oil extraction from orange peel via instant autovaporization. Chemical Engineering and Processing: Process Intensification. 2013;72:24-30.
  14. Rezzadori K, Benedetti S, Amante ER. Proposals for the residues recovery: Orange waste as raw material for new products. Food and bioproducts processing. 2012;90(4):606-14.
  15. Fernández-López J, Fernández-Ginés JM, Aleson-Carbonell L, Sendra E, Sayas-Barberá E, Pérez-Alvarez JA. Application of functional citrus by-products to meat products. Trends in Food Science & Technology. 2004;15(3-4):176-85.
  16. Al-Juhaimi FY, Ghafoor KA. Bioactive compounds, antioxidant and physico-chemical properties of juice from lemon, mandarin and orange fruits cultivated in Saudi Arabia. Pak. J. Bot. 2013;45(4):1193-6.
  17. Frassinetti S, Caltavuturo L, Cini M, Della Croce CM, Maserti BE. Antibacterial and antioxidant activity of essential oils from Citrus spp. Journal of Essential Oil Research. 2011;23(1):27-31.
  18. Jorge N, Silva AC, Aranha CP. Antioxidant activity of oils extracted from orange (Citrus sinensis) seeds. Anais da Academia Brasileira de Ciências. 2016;88:951-8.
  19. Kamal GM, Ashraf MY, Hussain AI, Shahzadi A, Chughtai MI. Antioxidant potential of peel essential oils of three Pakistani citrus species: Citrus reticulata, Citrus sinensis and Citrus paradisii. Pak. J. Bot. 2013;45(4):1449-54.
  20. Singh P, Shukla R, Prakash B, Kumar A, Singh S, Mishra PK, Dubey NK. Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DL-limonene. Food and Chemical Toxicology. 2010;48(6):1734-40.
  21. Trabelsi D, Ammar AH, Bouabdallah F. Antioxidant and Antimicrobial Activities of Essential Oils and Methanolic Extracts of Tunisian Citrus aurantium L. Journal of Environmental Science, Toxicology and Food Technology. 2014; 8(5): 18-27.
  22. Farahmandfar R, Tirgarian B, Dehghan B, Nemati A. Changes in chemical composition and biological activity of essential oil from Thomson navel orange (Citrus sinensis L. Osbeck) peel under freezing, convective, vacuum, and microwave drying methods. Food Science & Nutrition. 2020;8(1):124-38.
  23. Hassan IM, Ibrahim HM, Abdel Fattah A, Fattah A, Hamed AA. Citrus sinensis and Citrus aurantiifolia peel extracts: antibacterial, antioxidant activity and total phenolic. Int J Curr Microbiol App Sci. 2017;6:3983-98.
  24. Ramtin M, Massiha A, Khoshkholgh-Pahlaviani MR, Issazadeh K, Assmar M, Zarrabi S. Evaluation of the antibacterial activities of essential oils of Iris pseudacorus and Urtica dioica. Zahedan Journal of Research in Medical Sciences. 2014;16(3):35-9.
  25. Khanzadi S, Azizian A, Hashemi M, Azizzadeh M. Chemical Composition and Antibacterial Activity of the Emulsion and Nano-emulsion of Ziziphora clinopodioides Essential Oil against Escherichia coli O157: H7. Journal of Human, Environment, and Health Promotion. 2019;5(2):94.
  26. Banuree SA, Noori N, Gandomi H, Khanjari A, Karabagias IK, Faraki A, Ghadami F, Azizian A, Banuree SZ. Effect of Stevia rebaudiana aqueous extract and microencapsulation on the survivability of Bifidobacterium bifidum Bb‐12 and Lactobacillus acidophilus La‐5 in functional ice cream. International Journal of Food Science & Technology. 2022;57(12):7615-21.
  27. Ramezani Z, Zarei M, Raminnejad N. Comparing the effectiveness of chitosan and nanochitosan coatings on the quality of refrigerated silver carp fillets. Food Control. 2015;51:43-8.
  28. Gharibzahedi SM, Mohammadnabi S. Effect of novel bioactive edible coatings based on jujube gum and nettle oil-loaded nanoemulsions on the shelf-life of Beluga sturgeon fillets. International journal of biological macromolecules. 2017;95:769-77.
  29. Nam KC, Ahn DU. Use of antioxidants to reduce lipid oxidation and off-odor volatiles of irradiated pork homogenates and patties. Meat science. 2003;63(1):1-8.
  30. Baston O, Barna O. Raw chicken leg and breast sensory evaluation. Food Sci. Technol. 2010;11(1):25-30.
  31. Mirza MA, Baher Nik Z. The Role of deterpination on the essential oil composition of Citrus sinensis (L.) osbeck. Iranian Journal of Medicinal and Aromatic Plants Research. 2006;22(3):250-5.
  32. Dehghan B, Esmaeilzadeh Kenari R, Raftani Amiri Z. Investigate the Antioxidant Properties of Orange Peel Essential Oil (Citrus sinesis) on the Stability of Soybean Oil During Storage Conditions. Journal of Food Technology and Nutrition. 2019;16(3):73-90.
  33. Khan MM, Iqbal M, Hanif MA, Mahmood MS, Naqvi SA, Shahid M, Jaskani MJ. Antioxidant and antipathogenic activities of citrus peel oils. Journal of Essential Oil Bearing Plants. 2012;15(6):972-9.
  34. Institute of Standards and Industrial Research of Iran. ISO 9714, poltry meat.
  35. Adeli Milani M, Ghobadi Dana M, Ghanbarzadeh B, Alizadeh A, Ghasemi Afshar P. Effect of Gelatin/Hydroxypropyl-β-Cyclodextrin Bioactive Edible Coating Containing Nanoemulsion of Nettle Essential Oil on the Shelf Life of Turkey Meat. Journal of Food Technology and Nutrition. 2020;17(fall 2020):19-36.
  36. Mexis SF, Chouliara E, Kontominas MG. Combined effect of an oxygen absorber and oregano essential oil on shelf life extension of rainbow trout fillets stored at 4 C. Food microbiology. 2009; 26(6):598-605.
  37. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. International journal of food microbiology. 2004;94(3):223-53.
  38. Noshad M, Alizadeh Behbahani B, Jovindeh H, Rahmati Junidabad M, Ebrahimi Hemti Kikhah M, Qudsi Sheikh Jan M. Evaluation of the antimicrobial activity of Desfoli orange peel essence with and without therapeutic antibiotics on a number of pathogenic bacteria in laboratory conditions. Iranian Journal of Food Science and Industry. 2021; 18(111): 167-59.
  39. Fayaz far S, Khanjari A, Gandami Nasrabadi H, Akhundzade Basti A, Gholami F, Moghimi N. Studying the effect of Shirazi thyme essential oil on the storage time of fresh turkey sausage with normal packaging at refrigerated temperature. Journal of Veterinary Research. 2021; 7(6).323-33.
  40. Vardaka VD, Yehia HM, Savvaidis IN. Effects of Citrox and chitosan on the survival of Escherichia coli O157: H7 and Salmonella enterica in vacuum-packaged turkey meat. Food Microbiology. 2016; 58:128-34.
  41. Oraii F, Hosseini SE, Zorriehzahra SMJ, Safari, R. (). Determining the minimum inhibitory concentration of the ethanolic extract of orange peel and its effect on the flora of spoilage bacteria in elephant fish fillet (Huso huso) during storage in the refrigerator. Scientific Journal of Iranian Fisheries. 2019; 29(3). 25-36.
  42. Fan W, Chi Y, Zhang S. The use of a tea polyphenol dip to extend the shelf life of silver carp (Hypophthalmicthys molitrix) during storage in ice. Food chemistry. 2008;108(1):148-53.
  43. Taheri T, Fazlara A, Roomiani L. Effects of acetic acid on the microbial, chemical and sensorial attributes of turkey fillets during refrigerated storage. Journal of Food Microbiology. 2019;6(2):11-21.
  44. Alibeyghi T, Alizadeh Doughikollaee E, Zakipour Rahim Abadi E. Antioxidant effect of orange peel extract on the quality of common carp (Cyprinus carpio) fillet during refrigerated storage (4 C). Journal of fisheries. 2013; 66(2):185-97.
  45. Gil MI, Tomás-Barberán FA, Hess-Pierce B, Kader AA. Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. Journal of agricultural and food chemistry. 2002;50(17):4976-82.
  46. Sun XD, Holley RA. Antimicrobial and antioxidative strategies to reduce pathogens and extend the shelf life of fresh red meats. Comprehensive reviews in food science and food safety. 2012;11(4):340-54.
  47. Parish ME, Baum D, Kryger R, Goodrich R, Baum R. Fate of salmonellae in citrus oils and aqueous aroma. Journal of food protection. 2003 Sep 1;66(9):1704-7.
  48. Beigmohammadi F, Naseri HR, Mohammadi R, Sadeghi E. Production of edible film based on chitosan-gelatin, containing Ferulago angulate essential oil and evaluation of optical, sensory features and shelf life of packaged Turkey meat in it. Journal of Food Research. 2021;30(4):169-79.