The Effectiveness of Endurance Training and Nano Curcumin Supplementation on the Expression of Mir-21and P53 Genes in Brain Tumor Tissue in an Animal Model of Glioblastoma Multiform

Document Type : Research Paper


1 PhD Student, Department of Exercise Physiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

2 Associate Professor of Exercise Physiology Education &Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor, Department of Motor Behavior, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

4 Assistant Professor of Sports Physiology-Cardiovascular and Respiratory, Department of Sports Science, Jahrom University, Jahrom, Iran.

5 Associate Professor of Sports Physiology Research Center, Lifestyle Research Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.


Introduction: Glioblastoma multiforme is the foremost common harmful tumor of the central nervous system that specifically influences the brain and is resistent to common therapies such as surgery, radiotherapy and chemotherapy. The aim of this study was to examine the viability of perseverance preparing and Nano-curcumin supplementation on the expression of miR-21 and P53 qualities in brain tumor tissue in a creature demonstrate of glioblastoma multiforme. Methods: In this experiment, 35 8-week-old male Wistar rats were divided into seven groups with 5 rats each: healthy control group, 4-week-old healthy, control group cancer, 4-week-old cancer group and training group, Nano-curcumin group and training-Nano-curcumin group. Cancer cells were injected into the right frontal cortex of mice using a pump at a depth of 2.5 mm. One week later, mice entered the treadmill training program (4 weeks) and Nano-curcumin was administered orally at a dose of 80 mg/kg (28 days). Gene expression was measured using real-time fluorescent quantitative PCR and used for analysis.spss software. Results: The expression of miR-21 gene in the training group, Nano-curcumin, and training group Nano-curcumin was lower than that of the control cancer at 4 weeks (P = 0.001). Moreover, the expression of P53 gene in the Nano-curcumin training group and Nano-curcumin training group was higher in cancer cells and 4-week blood-eating cancer than in the control group (P = 0.001). Conclusion: Endurance training and curcumin administration appear to reduce tumor growth in mice with brain tumors by modulating the expression of miR-21 and p53 genes.


Main Subjects

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