The Effect of High Intensity Interval Training on Lipocalin 2 Gene Expression in Subcutaneous Adipose Tissue and Insulin Function in Obese Rats with Induced Diabetes

Document Type : Research Paper

Authors

1 Ph.D Student of Exercise Physiology, Islamic Azad University, Islamshahr Branch, Tehran, Iran.

2 Assistant Professor of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran.

4 Assistant Professor, Department of Physical Education and Sports Sciences, Parand Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction: Genetic evidence indicates the key role of transcription factors on insulin signaling pathways. The present study was performed with the aim of assess the effect of high intensity interval training (HIIT) on gene lipocalin-2 (LCP2) gene expression in subcutaneous adipose tissue, insulin resistance and glucose in obese rats with type 2 diabetic (T2D). Methods: For this purpose, 21 male Wistar rats aged 10 weeks of old were obesed by 8 weeks high-fat diet (HFD) and then 14 rats became T2D by STZ injection (25 mg/dL). The rats were divided into obese, control diabetic and HIIT diabetic groups. HIIT group participated in 8-week HIIT (5 sessions/weekly), and the control groups did not participate in the exercise program. 48 hours after the last training session, glucose and insulin resistance and gene LCN2 expression in subcutaneous adipose tissue were measured and compared by one-way ANOVA test. Results: Type 2 diabetes induction led to an increase in glucose and insulin resistance (P=0.001) as well as an increase in the expression of LCN2 (P=0.001) compared to the obese group. In other words, HIIT led to a significant decrease in glucose, insulin resistance (P=0.001) as well as a significant decrease in LCN2 expression (P=0.002) in subcutaneous adipose tissue compared to the diabetic control group. Conclusion: HIIT is associated with improvement of insulin resistance and glycemic profile in diabetic rats, and this can be imputed to the increase in LCN2 expression in subcutaneous adipose tissue in response to HIIT.

Keywords

Main Subjects

References

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Journal of Nutrition,Fasting and Health
Volume 12, Issue 2
June 2024
Pages 82-89

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History

  • Receive Date: 31 October 2023
  • Revise Date: 01 December 2023
  • Accept Date: 13 December 2023

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