The Effect of Interval Training on PPARy-GLUT4 Expression in Subcutaneous Adipose Tissue of Male Wistar Obese Rats

Document Type : Research Paper


1 Assistant Professor of Exercise Physiology, Department of Exercise physiology, Department of Physical Education and Sport Sciences Karaj Branch, Islamic Azad University, Karaj, Iran.

2 Assistant professor of Exercise Physiology, Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran.


Introduction: Genetic and metabolic dysfunctions are primary contributors to the onset and intensity of type 2 diabetes (T2D) through the promotion of insulin resistance. The objective of this research was to examine the impact of high-intensity interval training (HIIT) on the expression levels of PPARγ and GLUT4 in the subcutaneous adipose tissue, as well as its influence on insulin sensitivity among obese rats. Methods: Obesity induced by high fat diet (HDF) in 14 male wistar rats and then were divided accidentally into exercise (HIIT, n = 7) or control (n = 7) groups. The rats or exercise group were completed an 8 weeks HIIT (5 days / weekly) and control rats remained no-training. Fasting blood glucose, insulin sensitivity and PPARγ and GLUT4 gene expression in subcutaneous adipose tissue measured at 48 hours after lasting exercise session. Independent T test used to compare each variables between 2 groups. Results: HIIT resulted in significant decrease in fasting blood glucose (P = 0.001) and increase in insulin sensitivity (P = 0.001), PPARγ expression (P = 0.038) and GLUT4 expression (P = 0.019) in subcutaneous adipose tissue compared with control rats. Conclusion: HIIT can be improving insulin sensitivity in obese rats. This improvement may be attributed to increase PPARγ and GLUT4 expression in subcutaneous adipose tissue.


Main Subjects

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Articles in Press, Accepted Manuscript
Available Online from 07 February 2024
  • Receive Date: 09 December 2023
  • Revise Date: 08 January 2024
  • Accept Date: 07 February 2024