Comparative Study of Probiotic Properties of Lactobacillus Casei M3PM99 and Lactiplantibacillus Plantarum M1PM99 Isolated from Iranian Traditional Cheese

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Document Type : Research Paper

Authors

Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

Abstract

Introduction: this article aims to compare the in vitro probiotic potential of Lactobacillus casei and Lactiplantibacillus plantarum isolated from Semnan traditional cheese. Methods: The bacteria isolated from traditional cheese were sequenced based on the 16s rRNA gene and confirmed as L. casei and L.plantarum in the BLAST database. The probiotic properties were assessed in terms of resistance and growth in gastric juice and bile salts, survival rate in simulated gastrointestinal conditions, antimicrobial effect, hydrophobic properties and auto-aggregation and co-aggregation capabilities. Results: The Survival rate of L. plantarum (72.3%) was higher than L. casei (68%), and it exhibited higher growth rates at pH levels of 3.5 and 4.5. Additionally, L.plantarum demonstrated superior growth rate across various bile concentrations (0.3, 0.5 and 1%) (P <0.05). In simulated intestinal condition, L. casei was found to be more resistant than L. plantarum. The antimicrobial spot method revealed that L. casei had a stronger antagonistic effect against Staphylococcus areus, while L. plantarum showed greater efficacy against E. coli.  The well diffusion test confirmed the results of the previous antimicrobial method against these pathogens. Both lactobacillus isolates exhibited high percentage of auto-aggregation after 5 hours of incubation, ranging from 91.16% to 94.48%. Regarding co-aggregation, L. plantarum (42/02%) showed a significantly higher percentage than L. casei (21.02%) when co-aggregation with S.aureus (p<0.05). The hydrophobic properties of L.Casei (18.34%) were significantly lower than the those of L.plantarum (57.62%) (P<0.05). Conclusion: The results indicate that L. plantarum isolated from traditional cheese is a promising candidate for further probiotic studies.

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References

  1. Fijan S. Probiotics and Their Antimicrobial Effect. Microorganisms [Internet]. 2023; 11(2).

2.Bove P, Gallone A, Russo P, Capozzi V, Albenzio M, Spano G, et al. Probiotic features of Lactobacillus plantarum mutant strains. Applied microbiology and biotechnology. 2012;96:431-441.

3.Fischer SW, Titgemeyer F. Protective Cultures in Food Products: From Science to Market. Foods [Internet]. 2023; 12(7). https://doi.org/10.3390/foods12071541

4.Zhang Z, Lv J, Pan L, Zhang Y. Roles and applications of probiotic Lactobacillus strains. Applied Microbiology and Biotechnology. 2018;102(19):8135-8143.

5.Wang M, Gao Z, Zhang Y, Pan L. Lactic acid bacteria as mucosal delivery vehicles: a realistic therapeutic option. Applied microbiology and biotechnology. 2016;100:5691-5701.

6.Klein G, Pack A, Bonaparte C, Reuter G. Taxonomy and physiology of probiotic lactic acid bacteria. International Journal of Food Microbiology. 1998;41(2):103-25.

7.Al-Tawaha R, Meng C. Potential benefits of Lactobacillus plantarum as probiotic and its advantages in human health and industrial applications: A review. Adv Environ Biol. 2018;12:16-27.

8.Nicolescu CM, Bumbac M, Buruleanu CL, Popescu EC, Stanescu SG, Georgescu AA, et al. Biopolymers Produced by Lactic Acid Bacteria: Characterization and Food Application. Polymers (Basel). 2023;15(6).

9.Rokhtabnak N, Khaleghi M, Sasan H. Isolation and identification of Lactobacillus bacteria with probiotic potential from traditional dairy in Kerman. Iranian Journal of Medical Microbiology. 2016;10(1):24-34.

10.Hashemi SMB, Shahidi F, Mortazavi SA, Milani E, Eshaghi Z. Potentially probiotic Lactobacillus strains from traditional Kurdish cheese. Probiotics and antimicrobial proteins. 2014;6:22-31.

11.SARABI JM, RAHNAMA VP, KATE SM, Karazhyan R. Survivability of probiotic bacteria in simulated gastric and intestinal model.  J Food Sci Technol. 2017; 68(14): 331-340.

12.Byakika S, Mukisa IM, Byaruhanga YB, Muyanja C. A Review of Criteria and Methods for Evaluating the Probiotic Potential of Microorganisms. Food Reviews International. 2019;35(5):427-466.

 

13.Tabatabaei Yazdi F, Vasiee A, Alizadeh Behbahani B, Mortazavi SA. Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Kimchi Produced in Iran. muq-journal. 2015;9(5):11-22.

14.Sahadeva R, Leong S, Chua K, Tan C, Chan H, Tong E, et al. Survival of commercial probiotic strains to pH and bile. Int Food Res J. 2011;18(4):1515-22.

15.Ruiz L, Margolles A, Sánchez B. Bile resistance mechanisms in Lactobacillus and Bifidobacterium. Front Microbiol. 2013;4:396.

16.Farhangfar A, Gandomi H, Akhondzadeh Basti A, Misaghi A, Noori N. Study of growth kinetic and gastrointestinal stability of acid-bile resistant Lactobacillus plantarum strains isolated from Siahmazgi traditional cheese. Vet Res Forum. 2021;12(2):235-240.

17.Hassanzadazar H, Ehsani A, Mardani K, Hesari J. Investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from Koozeh cheese. Vet Res Forum. 2012;3(3):181-185.

18.Hwanhlem N, Salaipeth L, Charoensook R, Kanjan P, Maneerat S. Lactic Acid Bacteria from Gamecock and Goat Originating from Phitsanulok, Thailand: Isolation, Identification, Technological Properties and Probiotic Potential. J Microbiol Biotechnol. 2022;32(3):355-364.

19.Karimi S, Azizi F, Nayeb-Aghaee M, Mahmoodnia L. The antimicrobial activity of probiotic bacteria Escherichia coli isolated from different natural sources against hemorrhagic E. coli O157:H7. Electron Physician. 2018;10(3):6548-6553.

20.Lim S-M, Lee N-K, Paik H-D. Antibacterial and anticavity activity of probiotic Lactobacillus plantarum 200661 isolated from fermented foods against Streptococcus mutans. LWT. 2019;118:108840.

21.Bromberg R, Moreno I, Zaganini CL, Delboni RR, Oliveira Jd. Isolation of bacteriocin-producing lactic acid bacteria from meat and meat products and its spectrum of inhibitory activity. Brazilian Journal of Microbiology. 2004;35:137-144.

22.Krausova G, Hyrslova I, Hynstova I. In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains. Fermentation [Internet]. 2019; 5(4).

23.Sensoy I. A review on the food digestion in the digestive tract and the used in vitro models. Curr Res Food Sci. 2021;4:308-319.

24.Kongpracha P, Wiriyasermkul P, Isozumi N, Moriyama S, Kanai Y, Nagamori S. Simple but efficacious enrichment of integral membrane proteins and their interactions for in-depth membrane proteomics. Molecular & Cellular Proteomics. 2022;21(5).

25.Abdulla AA, Abed TA, Saeed A. Adhesion, autoaggregation and hydrophobicity of six Lactobacillus strains. British Microbiology Research Journal. 2014;4(4):381-391.

26.Ramos CL, Thorsen L, Schwan RF, Jespersen L. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food Microbiol. 2013;36(1):22-29.

  1. Nuraida L, Anggraeni D, dan Haryadi-Dewanti R. Adherence properties of lactic acid bacteria as probiotic candidates isolated from breast milk. Asian Journal of Food and Agro-Industry. 2012; 5: 500-511.

28.Isenring J, Geirnaert A, Lacroix C, Stevens MJ. Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota. BMC microbiology. 2021;21:1-13.

29.Talebi S, Makhdoumi A, Bahreini M. Isolation of Enterococcus sp. 7C37 with high probiotic potentials from Qaeni cheese, a diary fermented product in South Khorasan. Cellular and Molecular Research (Iranian Journal of Biology). 2020;32(4):459-468.

30.Grigoryan S, Bazukyan I, Trchounian A. Aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo: a potential probiotic strain. Probiotics and Antimicrobial Proteins. 2018;10:269-276.

31.Tuo Y, Yu H, Ai L, Wu Z, Guo B, Chen W. Aggregation and adhesion properties of 22 Lactobacillus strains. J Dairy Sci. 2013;96(7):4252-4257.

  1. Malfa P, Brambilla L, Giardina S, Masciarelli M, Squarzanti DF, Carlomagno F, et al. Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens. International Journal of Molecular Sciences. 2023;24(2):1323.
Journal of Nutrition,Fasting and Health

Articles in Press, Accepted Manuscript
Available Online from 02 March 2025

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  • Receive Date: 03 December 2024
  • Accept Date: 02 March 2025

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