Pomegranate Peel Extract Powder: Mitigating Cadmium Accumulation and Oxidative Damage in Common Carp Fillets

Document Type : Research Paper

Authors

1 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Introduction: Cadmium (Cd) contamination in aquatic environments threatens fish health by inducing oxidative stress and bioaccumulation. Natural antioxidants like pomegranate peel extract powder (PPEP) may help mitigate these effects through metal chelation and oxidative defense. This study evaluates PPEP’s potential to reduce Cd accumulation and oxidative damage in common carp. Methods: The protective effects of PPEP, as a natural dietary supplement, against Cd accumulation was evaluated by an inductively coupled plasma mass spectrometer, and the effect of PPEP on the oxidative effects of Cd in common carp was assessed via the determination of 2-thiobarbituric acid reactive substances (TBARs) and carbonyl content. Moreover, the bioaccumulation of some minerals (copper (Cu), magnesium (Mg), and zinc (Zn)) was also evaluated in the studied carps. Results: The feeding with different concentrations of PPEP (1, 2 and 4% wt) could significantly reduce the level of Cd in the fillet samples (P<0.05). Moreover, this supplementation also resulted in lower concentrations of Cu and Mg, while Zn was unaffected. Based on TBARs analysis, the levels of oxidation in Cd-supplemented samples were reduced (27-38%) by the PPEP treatments (P<0.05). However, no correlation was detected between protein oxidation and lipid peroxidation markers in the fish samples (P>0.05). Conclusion: This study demonstrates that PPEP effectively reduces cadmium accumulation and oxidative stress in common carp fillets. While PPEP mitigates lipid peroxidation, its impact on essential minerals like Cu and Mg requires further investigation. These findings support PPEP as a natural dietary strategy to improve fish health in aquaculture.

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  1. Ahmed E. Moneim A. Antioxidant activities of Punica granatum (pomegranate) peel extract on brain of rats. J Med Plants Res. 2012;6(2):195–9.
  2. Rakhnareh A, Safari O, Armament Y. Alteration of bacteria and yeast populations of Carassius auratus in response to diets containing Punica granatum extract. In: 7th National Conference of Iranian Fisheries. 2019.
  3. Abdel-Latif HMR, Abdel-Tawwab M, Khafaga AF, Dawood MAO. Dietary oregano essential oil improved the growth performance via enhancing the intestinal morphometry and hepato-renal functions of common carp (Cyprinus carpio L.) fingerlings. Aquaculture. 2020;526:735432.
  4. Taweel A, Shuhaimi-Othman M, Ahmad AK. Assessment of heavy metals in tilapia fish (Oreochromis niloticus) from the Langat River and Engineering Lake in Bangi, Malaysia, and evaluation of the health risk from tilapia consumption. Ecotoxicol Environ Saf. 2013;93:45–51.
  5. Maraschiello C, Sárraga C, García Regueiro JA. Glutathione Peroxidase Activity, TBARS, and α-Tocopherol in Meat from Chickens Fed Different Diets. J Agric Food Chem. 1999;47(3):867–72.
  6. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz A-G, et al. Determination of carbonyl content in oxidatively modified proteins. In: Oxygen Radicals in Biological Systems Part B: Oxygen Radicals and Antioxidants. Academic Press; 1990: 464–78.
  7. Badr HM, Mahmoud KA. Antioxidant activity of carrot juice in gamma irradiated beef sausage during refrigerated and frozen storage. Food Chem. 2011;127(3):1119–30.
  8. Zhang L, Gui P, Zhang Y, Lin J, Li Q, Hong H, et al. Assessment of structural, textural, and gelation properties of myofibrillar protein of silver carp (Hypophthalmichthys molitrix) modified by stunning and oxidative stress. LWT. 2019;102:142–9.
  9. Zaynab M, Al-Yahyai R, Ameen A, Sharif Y, Ali L, Fatima M, et al. Health and environmental effects of heavy metals. J King Saud Univ - Sci. 2022;34(1):101653.
  10. Vinodhini R, Narayanan M. Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). Int J Environ Sci Technol. 2008;5(2):179–82.
  11. Zhai Q, Narbad A, Chen W. Dietary strategies for the treatment of cadmium and lead toxicity. Nutrients. 2014;7(1):552-71.
  12. Gullón P, Astray G, Gullón B, Tomasevic I, Lorenzo JM. Pomegranate Peel as Suitable Source of High-Added Value Bioactives: Tailored Functionalized Meat Products. Molecules. 2020;25(12):2859.
  13. Mphahlele RR, Fawole OA, Makunga NP, Opara UL. Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activities of pomegranate peel. BMC Complement Altern Med. 2016;16(1):143.
  14. Barros Z, Salgado J, Melo P, Biazotto F. Enrichment of Commercially-Prepared Juice With Pomegranate (Punica granatum L.) Peel Extract as a Source of Antioxidants. J Food Res. 2014;3.
  15. Ozcan M, Dursun N, Sağlam C. Heavy Metals Bounding Ability of Pomegranate (Punica granatum) Peel in Model System. Int J Food Prop. 2011;14:550–6.
  16. Rasheed M, Tayh W. Removal of Heavy Metals from Wastewater Using Pomegranate Peel. IOP Conf Ser Mater Sci Eng. 2020;881:12187.
  17. Aksu DS, Sağlam YS, Yildirim S, Aksu T. Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats. Cell Mol Biol. 2017;63(10 SE-Original Research Articles):33–42.
  18. Čobanová K, Váradyová Z, Grešáková Ľ, Kucková K, Mravčáková D, Várady M. Does herbal and/or zinc dietary supplementation improve the antioxidant and mineral status of lambs with parasite infection?. Antioxidants. 2020;9(12):1172.
  19. Stef DS, Gergen I. Effect of mineral-enriched diet and medicinal herbs on Fe, Mn, Zn, and Cu uptake in chicken. Chem Cent J. 2012;6(1):19.
  20. Fiesel A, Ehrmann M, Geßner DK, Most E, Eder K. Effects of polyphenol-rich plant products from grape or hop as feed supplements on iron, zinc and copper status in piglets. Arch Anim Nutr. 2015;69(4):276–84.
  21. Kalay M, Canli M. Elimination of essential (Cu, Zn) and non-essential (Cd, Pb) metals from tissues of a freshwater fish Tilapia zilli. Turkish J Zool. 2000;24:429–36.
  22. Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Toxicology. 2011;283(2):65–87.
  23. Lönnerdal B. Dietary Factors Influencing Zinc Absorption. J Nutr. 2000;130(5):1378S-1383S.
  24. Okolie N, Okugbo O. A comparative study of malondialdehyde contents of some meat and fish samples processed by different methods. J Pharm Sci Innov. 2013;2:26–9.
  25. Rajeshkumar S, Liu Y, Ma J, Duan HY, Li X. Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L. Fish Shellfish Immunol. 2017;70:461–72.
  26. Orji OU, Awoke JN, Harbor C, Igwenyi IO, Obasi OD, Ezeani NN, et al. Ethanol leaf extract of Psychotria microphylla rich in quercetin restores heavy metal induced redox imbalance in rats. Heliyon. 2020;6(9):e04999.
  27. Jafari SS, Rahbarian R, Noghreie M. Protective effect of pomegranate peel (Punica granatum) ethanolic extract on common carp (Cyprinus carpio) exposed to cadmium. Iran Sci Fish J. 2023;31(6):95–105.
  28. Kumar D, Singh S, Singh AK, Rizvi SI. Pomegranate (Punica granatum) peel extract provides protection against mercuric chloride-induced oxidative stress in Wistar strain rats. Pharm Biol. 2013;51(4):441–6.
  29. Aksu DS, Saglam YS, Yildirim S, Aksu T. Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats. Cell Mol Biol. 2017;6(10):33–43.
  30. Milton Prabu S, Muthumani M, Shagirtha K. Protective effect of Piper betle leaf extract against cadmium-induced oxidative stress and hepatic dysfunction in rats. Saudi J Biol Sci. 2012;19(2):229–39.
  31. Vieira C, Guerra-Rivas C, Martínez B, Rubio B, Manso T. Effects of grape pomace supplementation on the diet of lactating ewes as compared to vitamin E on the meat shelf life of suckling lambs. Meat Sci. 2022;184:108666.
  32. Nardoia M, Ruiz-Capillas C, Casamassima D, Herrero AM, Pintado T, Jiménez-Colmenero F, et al. Effect of polyphenols dietary grape by-products on chicken patties. Eur Food Res Technol. 2018;244(2):367–77.
  33. Zhao C, Sakaguchi T, Fujita K, Ito H, Nishida N, Nagatomo A, et al. Pomegranate-derived polyphenols reduce reactive oxygen species production via SIRT3-Mediated SOD2 activation. Saretzki G, editor. Oxid Med Cell Longev. 2016;2016:2927131.
  34. Kolgiri V, Patil VW. Protein carbonyl content: a novel biomarker for aging in HIV/AIDS patients. Brazilian J Infect Dis. 2017;21(1):35–41.
  35. Ortuño J, Serrano R, Jordán MJ, Bañón S. Relationship between antioxidant status and oxidative stability in lamb meat reinforced with dietary rosemary diterpenes. Food Chem. 2016;190:1056–63.
  36. Adhami VM, Mukhtar H. Anti-Oxidants from Green Tea and Pomegranate for Chemoprevention of Prostate Cancer. Mol Biotechnol. 2007;37(1):52–7.