The Effect of Resistance Training with Black Seed Supplementation on Glycemic indices in Overweight Women

Document Type : Research Paper

Authors

Department of Physical Education and Sports Science, Gac.C., Islamic Azad University, Gachsaran, Iran

Abstract

Introduction: Today, obesity and insulin resistance are considered important factors in the development of metabolic diseases. This study aimed to investigate the effect of resistance training combined with black seed consumption on some glycemic indicators  in overweight women. Methods: In this quasi-experimental study, the researchers randomly divided 48 overweight and obese women into control, training, black seed, and training combined with black seed groups. The two training groups (with and without black seed administration) performed a resistance training program at 50–80% of 1RM, three sessions per week. The black seed group consumed two 1000 mg capsules daily. Tukey’s post-hoc test and one-way ANOVA were used to perform intergroup data analysis, while intragroup analysis was conducted using a paired sample t –test with version 26 of SPSS. Results: Compared to their pre-test values and the control group, all three experimental groups demonstrated a reduction in blood glucose, insulin, and insulin resistance index after eight weeks. A significant decrease was observed in the insulin resistance index (p = 0.0001) and insulin (p = 0.001) and glucose (p = 0.001) levels in the resistance training + black seed group. Additionally, the Training + black seed group showed a significant reduction in blood glucose (p = 0.001) and insulin resistance index (p = 0.01) compared to the resistance training group alone. Conclusion: Resistance training combined with black seed consumption can improve blood glucose, insulin levels, and insulin resistance index in overweight or obese individuals, who are predisposed to diabetes and cardiovascular diseases. 

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  1. Heymsfield S B, Wadden TA. Mechanisms, pathophysiology, and management of obesity, N Engl J Med. 2017; 376(3): 254-66. DOI: 10.1056/NEJMra1514009.
  2. Shrewsbury VA, Burrows T, Ho M, Jensen M, Garnett SP, Stewart L, et al. Update of the best practice dietetic management of overweight and obese children and adolescents: a systematic review protocol. JBI Database System Rev Implement Rep. 2018; 16(7), 1495-502. DOI: 10.11124/JBISRIR-2017-003603

3- Su, LiQiang, JinMei Fu, ShunLi Sun, GuangGao Zhao, Wei Cheng, ChuanChuan Dou, and MingHui Quan. "Effects of HIIT and MICT on cardiovascular risk factors in adults with overweight and/or obesity: A meta-analysis." PloS one 14, no. 1 (2019): e0210644.

  1. Aynalem, Shiferaw Birhanu, and Ayalew Jejaw Zeleke. "Prevalence of diabetes mellitus and its risk factors among individuals aged 15 years and above in Mizan‐Aman town, Southwest Ethiopia, 2016: a cross sectional study." International journal of endocrinology 2018, no. 1 (2018): 9317987.
  2. Türk, Y., W. Theel, M. J. Kasteleyn, F. M. E. Franssen, P. S. Hiemstra, A. Rudolphus, C. Taube, and G. J. Braunstahl. "High intensity training in obesity: a Meta‐analysis." Obesity science & practice 3, no. 3 (2017): 258-271.
  3. Lidegaard LP, Schwennesen N, Willaing I, Færch K. Barriers to and motivators for physical activity among people with Type 2 diabetes: patients' perspectives. Diabetic Medicine. 2016 Dec;33(12):1677-85.
  4. Li S, Yuan S, Zhang J, Xu F, Zhu F. The effect of periodic resistance training on obese patients with type 2 diabetic nephropathy. Scientific Reports. 2024 Feb 2;14(1):2761.
  5. Fathi R, Aslani Moghanjoughi S, Talebi E, Safarzadeh A, Seighal H. Effect of eight-week resistance training on plasma visfatin levels and its relation to insulin resistance in insulinresistant male rats. Iranian journal of Diabetes and Metabolism. 2015; 14(6): 390-8.
  6. Basami M, Ahmadizad S, Rahmani H, Nikseresht A. Effect of resistance exercise and its volume on plasma visfatin concentration and its relationship with insulin resistance index, interlukin-6 and growth hormone in men. J Sport Bioethics Sci. 2013; 5(9):33-41.

10.Saghebjoo M, Daste gardi S, Afzalpour ME, Hedayati M. Effects of aerobic and resistance training on plasma visfatin levels in overweight women. journal Koomesh. 2011;13(2):225- 32

11.Hosseini SA, Hamzavi K, Safarzadeh H, Salehi O. Interactive effect of swimming training and fenugreek (Trigonella foenum graecum L.) extract on glycemic indices and lipid profile in diabetic rats. Archives of Physiology and Biochemistry. 2023 Mar 4;129(2):349-53.

  1. Hosseini, Seyed Ali, Nasibeh Kazemi, Fateme Hajipour, and Sirous Farsi. "Hypoglycemic effects of nigella sativa extract and endurance training in streptozotocin induced diabetic rats." Iranian Journal of Diabetes and Obesity 9, no. 1 (2017): 25-31.
  2. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie SS, Al-Yahya AA, Al-Shabanah OA, Hafez MM, Nagi MN. Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxidative medicine and cellular longevity. 2010;3(4):254-61.
  3. Bamosa, Abdullah O., Huda Kaatabi, Fatma M. Lebdaa, Abdul-Muhssen Elq, and Ali Al-Sultanb. "Effect of Nigella sativa seeds on the glycemic control of patients with type 2 diabetes mellitus." Indian J Physiol Pharmacol 54, no. 4 (2010): 344-54.
  4. Heydari, M., and J. Dehghanizadeh. "Effect of aerobic exercise training and consumption of Nigella on the working memory of inactive middle-aged women." (2019): 20203074193.
  5. Shidfar F, Jazayeri S, Mousavi SN, Malek M, fateme HOSSEINI A, Khoshpey B. Does supplementation with royal jelly improve oxidative stress and insulin resistance in type 2 diabetic patients?. Iranian journal of public health. 2015 Jun;44(6):797.
  6. Azizbeigi K, Azarbayjani MA, Atashak S, Stannard SR. Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress. Res Sports Med 2015; 23(1):73-87.
  7. Patel V, Menezes H, Menezes C, Bouwer S, Bostick-Smith CA, Speelman DL. Regular mindful yoga practice as a method to improve androgen levels in women with polycystic ovary syndrome: a randomized, controlled trial. Journal of Osteopathic Medicine. 2020 May 1;120(5):323-35.
  8. Goto K, Ishii N, Kizuka T, Kraemer RR, Honda Y, Takamatsu K. Hormonal and metabolic responses to slow movement resistance exercise with different durations of concentric and eccentric actions. European journal of applied physiology. 2009 Jul;106(5):731-9.
  9. 20. Monroe, Jacob C., Kelly M. Naugle, and Keith E. Naugle. "Effect of acute bouts of volume-matched high-intensity resistance training protocols on blood glucose levels." The Journal of Strength & Conditioning Research 34, no. 2 (2020): 445-450
  10. Mehrzad Samarin M, Gholami M, Shahmohamadi F. The effect of eight weeks of ketogenic diet and resistance training on insulin resistance index and lipid profile in women with polycystic ovary syndrome. Journal of Applied Health Studies in Sport Physiology. 2024 Mar 20;11(1):67-81.
  11. Saremi A, Rajabi M. Effect of resistance training and omega-3 supplementation on metabolic parameters and anti-Mullerian hormone level in women with polycyctic ovarian syndrome. Pajoohandeh Journal. 2016 Aug 10;21(3):114-21.
  12. Gohari I, Khajehlandi A, Mohammadi A. Effect of high-intensity interval training with royal jelly consumption on serum levels of glucose, insulin, and insulin resistance index of overweight and obese middle-aged men: A quasi-experimental study. Jundishapur Journal of Chronic Disease Care. 2022 Jan 1;11(4).
  13. Park S, Hong SM, Lee JE, Sung SR. Exercise improves glucose homeostasis that has been impaired by a high-fat diet by potentiating pancreatic β-cell function and mass through IRS2 in diabetic rats. Journal of applied physiology. 2007 Nov;103(5):1764-71.
  14. Goodpaster BH, Katsiaras A, Kelley DE. Enhanced fat oxidation through physical activity is associated with improvements in insulin sensitivity in obesity. Diabetes 2003; 52: 2191-7.
  15. Parhizkar S, Latiff LA, Rahman SA, Dollah MA. Preventive effect of Nigella sativa on metabolic syndrome in menopause induced rats. J Med Plants Res. 2011 Apr 18;5(8):1478-84.
  16. Shah AS, Khan GM, Badshah A, Shah SU, Shah KU, Mirza SA, Khan KA. Nigella sativa provides protection against metabolic syndrome. African Journal of Biotechnology. 2012;11(48):10919-25.
  17. Jelodar GA, Maleki M, Motadayen MH, Sirus S. Effect of fenugreek, onion and garlic on blood glucose and histopathology of pancreas of alloxan-induced diabetic rats.
  18. Farah N, Benghuzzi H, Tucci M, Cason Z. The effects of isolated antioxidants from black seed on the cellular metabolism of A549 cells. Biomedical Sciences Instrumentation. 2005 Jan 1;41:211-6.
  19. Fararh KM, Atoji Y, Shimizu Y, Shiina T, Nikami H, Takewaki T. Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters. Research in veterinary science. 2004 Oct 1;77(2):123-9.
  20. Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin–nicotinamide induced diabetic rats. Life Sci 2009; 85(23-26): 830-834.
  21. Meddah B, Ducroc R, El Abbes Faouzi M, Eto B, Mahraoui L, Benhaddou-Andaloussi A, et al. Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats. J Ethnopharmacol 2009; 121(3): 419-424
  22. Benhaddou-Andaloussi A, Martineau LC, Spoor D, Vuong T, Leduc C, Joly E, Burt A, Meddah B, Settaf A, Arnason JT, Prentki M. Antidiabetic activity of Nigella sativa. Seed extract in cultured pancreatic β-cells, skeletal muscle cells, and adipocytes. Pharmaceutical biology. 2008 Jan 1;46(1-2):96-104.
  23. Heinonen MV, Laaksonen DE, Karhu T, Karhunen L, Laitinen T, Kainulainen S,et al. Effect of dietinduced weight loss on plasma apelin and cytokine levels in individuals with the metabolic syndrome. Nutr Metab Cardiovas. 2009; 19(9): 626-33
  24. Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine 2002; 9:69-74.
  25. Altan MF, Kanter M, Donmez S, Kartal ME, Buyukabs S. Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats. Acta Histochem 2007; 109: 304-14.

Articles in Press, Accepted Manuscript
Available Online from 10 August 2025
  • Receive Date: 25 June 2025
  • Revise Date: 08 August 2025
  • Accept Date: 09 August 2025