Short-term Effects of Pomegranate and Barberry Juice Consumption on Hemodynamic Parameters, and Blood Acidosis Following Force-Velocity Based Exercises in Male Athletes

Articles in Press

Document Type : Research Paper

Authors

1 Exercise Physiology Department, , Faculty of Physical Education and Sports Science, University of Guilan, Rasht, Iran

2 Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

The effects of using the combined supplement of pomegranate and barberry juice and the impact of the exercise sequence on hematological, hemodynamic, and acidity responses during and after sports preparation remain unclear. This study aimed to examine the responses of hematology, hemodynamics, and acidity to the sequence of intense anaerobic-resistance exercises after consuming the combined supplement of pomegranate and barberry juice in athletes. A total of 12 athletes, (average age of 24.33 ± 0.778 y, height of 169.75 ± 11.08 cm, weight of 66.93 ± 11.71 kg) participated in a double-blind crossover design, receiving either the combined supplement (220 mL of pomegranate and barberry juice) or a placebo in four separate trials. The exercise protocol included resistance-anaerobic exercises, performed sequentially in four different trials. The exercise protocol included anaerobic-resistance exercises, performed sequentially in four trials with different orders: power, velocity, and strength with supplement and placebo; power, strength, and velocity with supplement and placebo. The results showed that in all four trials, the consumption of the combined pomegranate and barberry juice supplement led to a significant decrease in MCHC (P = 0.007) and an increase in respiratory rate (P = 0.024) compared to the placebo. Although no significant differences were observed in hematological markers (HCT, MCH, HGB, WBC, LYM, PLT) between the groups, significant intra-group changes were found for each variable (P < 0.05). In terms of hemodynamic indicators (HR, SBP, DBP, MAP, SaO2, BR), no significant differences were found between the trials, but significant intra-group changes were noted (P < 0.05). Similarly, no significant changes were observed in the acidity markers (LA, pH, HCO3-) between the groups, but significant intra-group differences were present (P < 0.05). The findings of this study indicate that the Consumption of pomegranate and barberry juice supplement improves athletic performance and reduces metabolite accumulation. Additionally, the order of exercises affects physiological responses. These findings highlight the importance of supplementation and proper exercise sequencing in minimizing the negative effects of anaerobic training.

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References

  1. McCarthy JP, Agre JC, Graf BK, Pozniak MA, Vailas AC. Compatibility of adaptive responses with combining strength and endurance training. Medicine and science in sports and exercise. 1995;27(3):429-36.
  2. Ahmadizad S, El-Sayed MS. The acute effects of resistance exercise on the main determinants of blood rheology. Journal of sports sciences. 2005;23(3):243-9.
  3. Hu M, Finni T, Sedliak M, Zhou W, Alen M, Cheng S. Seasonal variation of red blood cell variables in physically inactive men: effects of strength training. International journal of sports medicine. 2008;29(07):564-8.
  4. Morici G, Zangla D, Santoro A, Pelosi E, Petrucci E, Gioia M, et al. Supramaximal exercise mobilizes hematopoietic progenitors and reticulocytes in athletes. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2005;289(5):R1496-R503.
  5. El-Sayed MS, Sale C, Jones P, Chester M. Blood hemostasis in exercise and training. Medicine and science in sports and exercise. 2000;32(5):918-25.
  6. Alam T, Rahman SN, Alam T, Habib N, Umar BU, Banna QR, et al. Effect of physical exercise on some hematological parameters in female athletes in Bangladesh. JNMA J Nepal Med Assoc. 2014;52(195):892-6.
  7. Lorzadeh E, Heidary Z, Mohammadi M, Nadjarzadeh A, Ramezani-Jolfaie N, Salehi-Abargouei A. Does pomegranate consumption improve oxidative stress? A systematic review and meta-analysis of randomized controlled clinical trials. Clinical Nutrition ESPEN. 2022;47:117-27.
  8. Ammar A, Bailey SJ, Chtourou H, Trabelsi K, Turki M, Hökelmann A, et al. Effects of pomegranate supplementation on exercise performance and post-exercise recovery in healthy adults: a systematic review. British Journal of Nutrition. 2018;120(11):1201-16.
  9. Kulkarni S, Dhir A. Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2010;24(3):317-24.
  10. Belyani S, Kazeminasab F, Niazi M, Bagheri R, Hesari MM, Rosenkranz SK, et al. The Effects of Pomegranate Supplementation on Markers of Exercise-Induced Muscle Damage: A Systematic Review and Meta-Analysis. Current Developments in Nutrition. 2025;9(2).
  11. Fiorenza M, Hostrup M, Gunnarsson TP, Shirai Y, Schena F, Iaia FM, et al. Neuromuscular fatigue and metabolism during high-intensity intermittent exercise. Medicine and science in sports and exercise. 2019;51(8):1642-52.
  12. Farinatti PT, da Silva NS, Monteiro WD. Influence of exercise order on the number of repetitions, oxygen uptake, and rate of perceived exertion during strength training in younger and older women. The Journal of Strength & Conditioning Research. 2013;27(3):776-85.
  13. He F, Li J, Liu Z, Chuang C-C, Yang W, Zuo L. Redox mechanism of reactive oxygen species in exercise. Frontiers in physiology. 2016;7:486.
  14. Madan K, Bhardwaj P, Thareja S, Gupta SD, Saraya A. Oxidant stress and antioxidant status among patients with nonalcoholic fatty liver disease (NAFLD). Journal of clinical gastroenterology. 2006;40(10):930-5.
  15. Miszko TA, Cress ME, Slade JM, Covey CJ, Agrawal SK, Doerr CE. Effect of strength and power training on physical function in community-dwelling older adults. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2003;58(2):M171-M5.
  16. Trombold JR, Reinfeld AS, Casler JR, Coyle EF. The effect of pomegranate juice supplementation on strength and soreness after eccentric exercise. J Strength Cond Res. 2011;25(7):1782-8.
  17. de Salles BF, Simão R, Miranda F, Novaes Jda S, Lemos A, Willardson JM. Rest interval between sets in strength training. Sports Med. 2009;39(9):765-77.
  18. Grgic J, Lazinica B, Schoenfeld BJ, Pedisic Z. Test-Retest Reliability of the One-Repetition Maximum (1RM) Strength Assessment: a Systematic Review. Sports Med Open. 2020;6(1):31.
  19. Hernández Belmonte A. Effects of resistance training modality on physical performance and muscle structure. Proyecto de investigación. 2023.
  20. Arazi H, Salehi A, Hosseini Y, Jahanmahin M. The response of hematological factors to a circuit resistance training program with various intensities in athlete male students. Scientific Journal of Iranian Blood Transfusion Organization. 2012;9(1).
  21. Stefanović R, Ristić B, Drvenica I. Red blood cell adaptations induced by exercise. Srpski medicinski časopis Lekarske komore. 2024;5(2):215-27.
  22. Manthou E, Georgakouli K, Deli CK, Sotiropoulos A, Fatouros IG, Kouretas D, et al. Effect of pomegranate juice consumption on biochemical parameters and complete blood count. Experimental and therapeutic medicine. 2017;14(2):1756-62.
  23. Asghar Z, Masood Z. Evaluation of antioxidant properties of silymarin and its potential to inhibit peroxyl radicals in vitro. Pakistan Journal of Pharmaceutical Sciences. 2008;21(3).
  24. Özbay S, Ulupinar S. The Effect of Moderate-Intensity Technical Training and Resistance Training on Selected Hematological Parameters of Elite Wrestlers. Universal Journal of Educational Research. 2018;6(11):2679-82.
  25. Moradnia M, Mohammadkhani N, Azizi B, Mohammadi M, Ebrahimpour S, Tabatabaei-Malazy O, et al. The power of Punica granatum: A natural remedy for oxidative stress and inflammation; a narrative review. Journal of Ethnopharmacology. 2024:118243.
  26. Sadeghian Rad M, Mehrbani J. Short-term effect of caffeine and beetroot juice supplementation on antioxidants and performance in endurance athletes. Journal of Applied Exercise Physiology. 2021;17(34):3-4.
  27. Ammar A, Turki M, Hammouda O, Chtourou H, Trabelsi K, Bouaziz M, et al. Effects of pomegranate juice supplementation on oxidative stress biomarkers following weightlifting exercise. Nutrients. 2017;9(8):819.
  28. Chiron F, Thomas C, Bardin J, Mullie F, Bennett S, Chéradame J, et al. Influence of Ingestion of Bicarbonate-Rich Water Combined with an Alkalizing or Acidizing Diet on Acid-Base Balance and Anaerobic Performance. Journal of Human Kinetics. 2024;93:105.
  29. Beaver WL, Wasserman K, Whipp BJ. Bicarbonate buffering of lactic acid generated during exercise. Journal of Applied Physiology. 1986;60(2):472-8.
  30. Gough LA, Rimmer S, Osler CJ, Higgins MF. Ingestion of sodium bicarbonate (NaHCO3) following a fatiguing bout of exercise accelerates postexercise acid-base balance recovery and improves subsequent high-intensity cycling time to exhaustion. International journal of sport nutrition and exercise metabolism. 2017;27(5):429-38.
  31. Faizal P, Satheesan B, Vinod B, Augusti KT. Evaluation of antioxidant, lipid peroxidation and toxic effects after pomegranate intake in healthy human volunteers. International Journal of Clinical Medicine. 2017;8(1):12-20.
  32. Talbott SM, Hantla D, Capelli B, Ding L, Li Y, Artaria C. Effect of Astaxanthin supplementation on psychophysiological heart-brain axis dynamics in healthy subjects. Functional Foods in Health and Disease. 2019;9(8):521-31.
  33. Zhou K, Shang Z, Yuan C, Guo Z, Wang Y, Bao D, et al. Can molecular hydrogen supplementation enhance physical performance in healthy adults? A systematic review and meta-analysis. Frontiers in Nutrition. 2024;11:1387657.
  34. Menezes EF, Peixoto LG, Teixeira RR, Justino AB, Puga GM, Espindola FS. Potential benefits of nitrate supplementation on antioxidant defense system and blood pressure responses after exercise performance. Oxidative medicine and cellular longevity. 2019;2019(1):7218936.
  35. conductor M, Farshad G, JAVAD K, Hussain AT, Mandana G. The Effect of Short-Term Caffeine and Beet Juice Supplementation with High and Low Doses and Placebo on Vasodilatory Vasodilation Indices and Exercise Performance in Elite Male Runners. 2021.
  36. Ahmad A, Javad M, TAHEREH H, Zainab Sa-I, Maryam MS. The Protective Effect of Aerobic Training with Pomegranate Juice Concentrate on Heart Damage Indices in Type 2 Diabetic Women. 2019.
Journal of Nutrition,Fasting and Health

Articles in Press, Accepted Manuscript
Available Online from 02 September 2025

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History

  • Receive Date: 16 April 2025
  • Revise Date: 09 August 2025
  • Accept Date: 02 September 2025

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