Journal of Nutrition,Fasting and Health

Journal of Nutrition,Fasting and Health

Combined Training Attenuates Lipid Dysregulation and Liver Injury Markers in a Rat Model of Diet‑Induced NAFLD

Articles in Press

Document Type : Research Paper

Authors
Seyed Ali Mirghani 1
Mania Roozbayani 2
Saleh Rahmati 3
Lida Moradi 4
1 Department of Physical Education and Sports Sciences, CT.C., Islamic Azad University Tehran, Iran.
2 Department of Exercise Physiology, CT.C., Islamic Azad University, Tehran, Iran.
3 Department of Physical Education, Par.C., Islamic Azad University, Pardis, Iran.
4 Department of Physical Education and Sports Sciences, NT.C., Islamic Azad University, Tehran, Iran.
10.22038/jnfh.2026.92075.1604
Abstract
Introduction: This study aimed to evaluate the effects of combined exercise training on dyslipidemia and liver injury biomarkers in rats with diet-induced nonalcoholic fatty liver disease (NAFLD).
Methods: Twenty male Wistar rats were allocated to three groups: normal control, NAFLD, and NAFLD plus combined training. NAFLD was induced by feeding the rats a high-fat, high-fructose diet for 15 weeks. After NAFLD was confirmed, the combined training group completed an eight-week exercise program. Serum lipid parameters and hepatic enzyme activities were measured to assess metabolic and hepatic changes. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test.
Results: Compared with the NAFLD group, rats that underwent combined training showed significant reductions in triglyceride (TG) and aspartate aminotransferase (AST) levels (P<0.001). However, combined training did not significantly alter low-density lipoprotein (LDL) or alkaline phosphatase (ALP) levels (P≥0.05).
Conclusion: Combined training improved the lipid profile and reduced markers of liver injury in rats with diet-induced NAFLD, supporting its potential as a nonpharmacological strategy for attenuating NAFLD-related metabolic disturbances. Larger studies are needed to confirm these findings.
Keywords
High-Intensity Interval Training
Resistance Training
Hepatic Enzymes
NAFLD Rat Model
Subjects
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Journal of Nutrition,Fasting and Health

Articles in Press, Accepted Manuscript
Available Online from 09 September 2026

  • Receive Date 19 October 2025
  • Revise Date 19 July 2026
  • Accept Date 26 July 2026