Effects of Microwave Radiation, Organic Acid and Salt Combination on Survival of Pseudomonas Aeruginosa Inoculated In Veal Meat Stored In Refrigerator

Document Type : Research Paper

Authors

1 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Professor Department of Faculty of Veterinary Medicine Ferdowsi University Of Mashhad , Mashhad , Iran

3 Department of Clinical Sciences. Faculty of Veterinary Medicine. Ferdowsi University Of Mashhad, Mashhad, Iran

Abstract

Introduction: Different species of Pseudomonas bacteria are found in abundance in environment. Although they are weak pathogens, they have great importance in food hygiene and human health because they are psychrotrophic bacteria, can grow and proliferate at refrigerated temperatures, and can produce proteolytic and lipolytic enzymes. Method: In this study, the simultaneous effect of different doses of microwave radiation and different concentrations of salt and lactic acid on Pseudomonas aeruginosa inoculated onto veal meat pieces. For this purpose, 108 samples were evaluated in 9 treatments on days 0, 3, 6, 9, 12, and 15 during chilled storage in -4⁰ C. The control group was treated with distilled water. The bacterial count was done in Pseudomonas Agar medium and that showed that different concentrations of acid, salt, and microwave exposure time and their interactions had a significant effect on the mean logarithm number of bacteria during the refrigerating period. Result: In this study, the interaction between microwave radiation, organic acid and salt cause to more reduction in the count of bacteria than the control group. There was a significant difference between the number of bacteria under acid and salt treatments and the control group (p<0.001)In this study, by controlling the effect of salt concentration and microwave duration, using 2.5 and 5% lactic acid led to reduce the logarithm of the bacteria during the refrigeration  period in comparison with the acid-free samples (P<0.001) and as well as, 5% acid significantly decrease the logarithm of the number of bacteria during the refrigeration  period compared to the samples treated with 2.5% acid (P <0.001). Also, by controlling the impact of the concentration if the acid and microwave duration, using 4 and 6% salt significantly decrease the logarithm of the number of bacteria during the refrigeration period compared to non-salty samples (p <0.001). Samples containing of 6% and 4% salt did not indicate a significant difference in the logarithm of the number of bacteria during the refrigeration period (p = 0.89). Also, the microwave duration of 9 s (65 °C) significantly decreases the logarithm of the bacteria compared to 7 (55 °C), 5 (45 °C) and 3 s (30 °C) (P <0.001). Also, using lactic acid (5%) and salt (6%) the microwave duration was reduced from 9 s (65 °C) to 5 s (45 °C) in order to complete elimination of bacteria. The results were analyzed by mixed repeated measure ANOVA (SPSS version 21). Conclusion: In summary, the results of this study showed that the use of organic acid, salt, and microwave irradiation on pseudomonas aeruginosa, which was inoculated into meat parts during storage in the refrigerator, reduced the bacterial count during this period. In this study, the combination of microwave radiation, organic acid and salt concentration caused a greater reduction in bacteria count compared to the control group, and there was a significant difference between the bacterial count when each these three factors were used comparing with control group.

Keywords

References

1.  Poole K. Pseudomonas aeruginosa: resistance to the max. Frontiers in microbiology. 2011; 2:65.
2.  Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical microbiology reviews. 2009;22(4):582-610.
3.  Eckmanns T, Oppert M, Martin M, Amorosa R, Zuschneid I, Frei U, et al. An outbreak of hospital-acquired Pseudomonas aeruginosa infection caused by contaminated bottled water in intensive care units. Clinical Microbiology and Infection. 2008;14(5):454-8.
4.  Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends in microbiology. 2011;19(8):419-26.
5.  Kipnis E, Sawa T, Wiener-Kronish J. Targeting mechanisms of Pseudomonas aeruginosa pathogenesis. Medicine et maladies infectieuses. 2006;36(2):78-91.
6.  Jefferies J, Cooper T, Yam T, Clarke S. Pseudomonas aeruginosa outbreaks in the neonatal intensive care unit–a systematic review of risk factors and environmental sources. Journal of medical microbiology. 2012;61(8):1052-61.
7.  Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, et al. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 2006;312(5779):1526-30.
8.  Mesaros N, Nordmann P, Plésiat P, Roussel-Delvallez M, Van Eldere J, Glupczynski Y, et al. Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clinical microbiology and infection. 2007;13(6):560-78.
9.  Isara A, Isah E, Lofor P, Ojide C. Food contamination in fast food restaurants in Benin City, Edo State, Nigeria: Implications for food hygiene and safety. Public health. 2010;124(8):467-71.
10. Montville TJ, Matthews KR. Food microbiology: an introduction: ASM Press; 2007.
11. Rahman MS. Food Preservation.  Handbook of Food Preservation, Second Edition: CRC press; 2007. p. 14-29.
12. Cotter PD, Hill C, Ross RP. Food microbiology: bacteriocins: developing innate immunity for food. Nature Reviews Microbiology. 2005;3(10):777.
13. Cleveland J, Montville TJ, Nes IF, Chikindas ML. Bacteriocins: safe, natural antimicrobials for food preservation. International journal of food microbiology. 2001;71(1):1-20.
14. Tiwari BK, Valdramidis VP, O’Donnell CP, Muthukumarappan K, Bourke P, Cullen P. Application of natural antimicrobials for food preservation. Journal of agricultural and food chemistry. 2009;57(14):5987-6000.
15. Piper P, Calderon CO, Hatzixanthis K, Mollapour M. Weak acid adaptation: the stress response that confers yeasts with resistance to organic acid food preservatives. Microbiology. 2001;147(10):2635-42.
16. Hazan R, Levine A, Abeliovich H. Benzoic acid, a weak organic acid food preservative, exerts specific effects on intracellular membrane trafficking pathways in Saccharomyces cerevisiae. Applied and environmental microbiology. 2004;70(8):4449-57.
17. Thomas LV, Wimpenny J. Investigation of the effect of combined variations in temperature, pH, and NaCl concentration on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus. Applied and environmental microbiology. 1996;62(6):2006-12.
18. Brul S, Coote P. Preservative agents in foods: mode of action and microbial resistance mechanisms. International journal of food microbiology. 1999;50(1-2):1-17.
19. de Mendonça AJ, Vaz MI, de Mendonça DI. Activity coefficients in the evaluation of food preservatives. Innovative Food Science & Emerging Technologies. 2001;2(3):175-9.
20. Quintavalla S, Vicini L. Antimicrobial food packaging in meat industry. Meat science. 2002;62(3):373-80.
21. Knorr D. Technology aspects related to microorganisms in functional foods. Trends in Food Science & Technology. 1998;9(8-9):295-306.
22. Merritt ME, Donaldson JR. Effect of bile salts on the DNA and membrane integrity of enteric bacteria. Journal of medical microbiology. 2009;58(12):1533-41.
23. Woo I-S, Rhee I-K, Park H-D. Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure. Applied and Environmental Microbiology. 2000;66(5):2243-7.
24. Chandrasekaran S, Ramanathan S, Basak T. Microwave food processing—A review. Food Research International. 2013;52(1):243-61.
25. de Pomerai DI, Smith B, Dawe A, North K, Smith T, Archer DB, et al. Microwave radiation can alter protein conformation without bulk heating. FEBS letters. 2003;543(1-3):93-7.
26. Celandroni F, Longo I, Tosoratti N, Giannessi F, Ghelardi E, Salvetti S, et al. Effect of microwave radiation on Bacillus subtilis spores. Journal of Applied Microbiology. 2004;97(6):1220-7.
27. Gracey JF. Meat hygiene: Baillière Tindall; 1986.
28. Hathaway S. Risk analysis and meat hygiene. REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES. 1993; 12:1265-.
29. CHUNG K-T, DICKSON JS, GROUSE JD. Attachment and proliferation of bacteria on meat. Journal of Food Protection. 1989;52(3):173-7.
30. Al-Haddad KS, Al-Qassemi RA, Robinson R. The use of gaseous ozone and gas packaging to control populations of Salmonella infantis and Pseudomonas aeruginosa on the skin of chicken portions. Food Control. 2005;16(5):405-10.
31. Peck MW, Goodburn KE, Betts RP, Stringer SC. Assessment of the potential for growth and neurotoxin formation by non-proteolytic Clostridium botulinum in short shelf-life commercial foods designed to be stored chilled. Trends in Food Science & Technology. 2008;19(4):207-16.
32. Liu PV. The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. III. Identity of the lethal toxins produced in vitro and in vivo. Journal of Infectious Diseases. 1966;116(4):481-9.
33. Liu PV. Extracellular toxins of Pseudomonas aeruginosa. Journal of Infectious Diseases. 1974;130(Supplement): S94-S9.
34. Aubry C, Goulard C, Nahori M-A, Cayet N, Decalf J, Sachse M, et al. OatA, a peptidoglycan O-acetyltransferase involved in Listeria monocytogenes immune escape, is critical for virulence. Journal of Infectious Diseases. 2011;204(5):731-40.
35. Davidson PM, Taylor TM, Schmidt SE. Chemical preservatives and natural antimicrobial compounds.  Food microbiology: American Society of Microbiology; 2013. p. 765-801.
36. Koyuncu F. Organic acid composition of native black mulberry fruit. Chemistry of natural compounds. 2004;40(4):367-9.
37. Casaburi A, Piombino P, Nychas GJ, Villani F, Ercolini D. Bacterial populations and the volatilome associated to meat spoilage. Food microbiology. 2015 Feb 1; 45:83-102.
38. Davidson, P. M., Taylor, T. M., & Schmidt, S. E. (2013). Chemical preservatives and natural antimicrobial compounds. In Food microbiology (pp. 765-801). American Society of Microbiology.
39. Koyuncu, F. (2004). Organic acid composition of native black mulberry fruit. Chemistry of natural compounds, 40(4), 367-369.
40. Theron MM, Lues JF. Organic acids and meat preservation: a review. Food Reviews International. 2007;23(2):141-58.
41. Russell NJ, Gould GW. Food preservatives: Springer Science & Business Media; 2003.
42 Foster JW. Hall HK. Adaptive acidification tolerance response of salmonella typhimurium. Journal of Bacteriology.1990: 172,771-778. Ndraha N, Hsiao HI, Vlajic J, Yang MF, Lin HT. Time-temperature abuse in the food cold chain: Review of issues, challenges, and recommendations. Food Control. 2018 Jul 1; 89:12-21.
43. Mani-Lopez E, Garcia HS, López-Malo A. Organic acids as antimicrobials to control Salmonella in meat and poultry products. Food Research International. 2012 Mar 1;45(2):713-21
44. Jay JM, Loessner MJ, Golden DA. Modern food microbiology. Springer Science & Business Media; 2008 Feb 5.
45. Ndraha N, Hsiao HI, Vlajic J, Yang MF, Lin HT. Time-temperature abuse in the food cold chain: Review of issues, challenges, and recommendations. Food Control. 2018 Jul 1; 89:12-21.
46. Arnaut-Rollier I, De Zutter L, Van Hoof J. Identities of the Pseudomonas spp. in flora from chilled chicken. International journal of food microbiology. 1999 May 1;48(2):87-96.
47.Ghazvini A, Rudsari HV, Takami GA, Masuleh AR, Ashourpour A. Disinfection efficiency of three anti-fungal agents (nanosil, Chloramine-T and hydrogen peroxide) on Persian sturgeon (Acipenser persicus, Borodin 1897) larvae. International Journal of Biology. 2012;4(1):138.
48. Thomas, L.V. and Wimpenny, J.W., 1996. Investigation of the effect of combined variations in temperature, pH, and NaCl concentration on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus. Applied and environmental microbiology, 62(6), pp.2006-2012.
49. 48. Zeinali T, Khandvi S, Jamshidi A, Azizzadeh M. Growth response of Salmonella typhimurium as a function of temperature, pH, organic and inorganic acids, and NaCl concentration. Iranian Journal of Veterinary Science and Technology. 2014 Apr 1;4(1):9-18.
50. Le Marc Y, Plowman J, Aldus CF, Munoz-Cuevas M, Baranyi J, Peck MW. Modelling the growth of Clostridium perfringens during the cooling of bulk meat. International journal of food microbiology. 2008 Nov 30;128(1):41-50.
51. Brudzinski L, Harrison MA. Influence of incubation conditions on survival and acid tolerance response of Escherichia coli O157: H7 and non-O157: H7 isolates exposed to acetic acid. Journal of Food Protection. 1998 May;61(5):542-6.
52. Presser Ka, et al. Modelling the growth rate of Escherichia coli as a function of pH and lactic acid concentration. Individual and Combined Effects of pH and Lactic Acid Concentration on Listeria innocua Inactivation: Appl Environ Microbiology. 1998;64:1773- 1779.
53. Juven BJ. Et al. Influence of organic juice composition on the thermal resistance of spoilage yeasts. Journal of food science. 1978;43: 1074-1080.
54. Goncalves AC, Almeida RC, Alves MA, Almeida PF. Quantitative investigation on the effects of chemical treatments in reducing Listeria monocytogenes populations on chicken breast meat. Food control. 2005 Sep 1;16(7):617-22.

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History

  • Receive Date: 16 March 2019
  • Revise Date: 29 April 2019
  • Accept Date: 21 October 2019

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