Introduction: Today, the use of medicinal herbs to prevent and slow down the progression of Parkinson's disease has attracted the attention of researchers. This research seeks to answer the question of whether the extract of leaf of olive can be used as an appropriate alternative to levodopa in reducing the symptoms of Parkinson's. Methods: In this study, 60 small mice were assigned in 6 groups including control, Parkinson, levodopa, and 3 groups receiving the hydroalcoholic extract of olive leaf in three doses of 50,100 and 200 mg/kg. All groups except the control group received paroxetine for 19 hours at subcutaneous intervals of 48 hours. After induction of Parkinson's by Rotenone, the drug group received levodopa ½ mg/kg of mice weight, and the extract groups received an intraperitoneal injection in three doses of 50, 100 and 200 mg/kg of mice, respectively, and 55 minutes after injection, each mice was individually evaluated behavioral by both Rearing test and bar test, and standard assessment indicators were checked and recorded through the analysis the review and recorded. Results: The results show that extract in a dose of 100 mg/kg in bar test compared with the drug group and other doses of the extract significantly increased the holding time of the bar by the mouse which indicating a reduction in the symptoms of Parkinson's. Conclusion: Based on the results, it seems that the hydroalcoholic extract of the olive leaf with a dose of 100 mg/kg of mice weight can be considered as a suitable alternative to levodopa in reducing Parkinson's symptoms.