Characterization of White Chocolate Enriched with Free or Encapsulated Pomegranate Extract

Document Type : Research Paper


Assistant Professor of Food Science, Department of Food Science and Technology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran



Introduction: Chocolate is a popular food product. White chocolate is produced extensively and has lower functionality compared to other chocolates. The present study aimed to characterize white chocolate enriched with free or encapsulated pomegranate extract. Methods: To increase the functionality of white chocolate, pomegranate extract was added in the free or encapsulated forms at various concentrations (1, 2, 3, 4, and 5 g/200 g). The coacervation was performed for the encapsulation of pomegranate extract. The quality parameters of the chocolate samples were also evaluated, including the color index, melting behavior, flow behavior (rheometer and scanning electron microscopy), particle size distribution, texture analysis, and organoleptic properties. Other analysis included the determination of the total phenolic compounds and antioxidant properties of the samples. Results: The addition of pomegranate extract (free/encapsulated) decreased Tonset, Tpeak, and DH of white chocolate compared to the control samples. In addition, the added pomegranate extract reduced the casson yield stress, while it increased casson viscosity. The extract also changed the color indices and particle size distribution (P<0.05). The white chocolate samples containing pomegranate extract (free/encapsulated) had a higher phenolic content and antioxidant activity than the control samples. Moreover, sensory properties were affected by chocolate formulation, and the samples containing encapsulated pomegranate extract had superior organoleptic properties compared to the samples with free pomegranate extract. Conclusion: According to the results, free or encapsulated pomegranate extract could enhance the functionality of white chocolate in term of antioxidant activity. Furthermore, organoleptic properties were affected by chocolate formulation, and the samples containing encapsulated pomegranate extract had better organoleptic properties compared to those containing the free form of the extract.


  1. Lončarević I, Pajin B, Fišteš A, Tumbas Šaponjac V, Petrović J, Jovanović P, Vulić J, Zarić D. Enrichment of white chocolate with blackberry juice encapsulate: Impact on physical properties, sensory characteristics and polyphenol content. LWT - Food Sci. Technol  2018; 92:458–464.
  2. Lončarević I, Pajin B,  Petrović J, Danica Zarić D,  Šaponjac VT, Fišteš A, Petar Jovanović P. The physical properties, polyphenol content and sensory characteristics of white chocolate enriched with black tea extract. Food in Health and Disease, scientific-professional journal of nutrition and dietetics 2019; 8 (2) 83-88.
  3.  Lončarević I,  Pajin B,   Šaponjac VT,   Petrović J,   Vulić J,   Fišteš A,  Jovanović P. Physical, sensorial and bioactive characteristics of white chocolate with encapsulated green tea extract.  Quality and shelf life of white chocolate with green tea encapsulate. J Sci Food Agric 2019; 99(13): 5834-5841.
  4. Ozkan G, Franco P, De Marco I, Xiao J, Capanoglu E. A review of microencapsulation methods for food antioxidants: Principles, advantages, drawbacks and applications. Food Chem 2019; 272 :494–506.
  5. Ballesteros L F, Ramirez M J, Orrego C E, Teixeira J A, Mussatto S I. Encapsulation of antioxidant phenolic compounds extracted from spent coffee grounds by freeze-drying and spray-drying using different coating materials. Food Chem 2017; 237:623-631.
  6. Longtin R. The pomegranate: nature's power fruit? J Natl Cancer Inst. 2003; 95, 346–348.
  7. Gil M I, Tomas-Barberan F A, Hess-Pierce B, Holcroft D M, Kader A A. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem 2000; 48, 4581–4589.
  8. Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, et al. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr 2000; 71, 1062–1076.
  9. Jurenka J.  Therapeutic Applications of Pomegranate (Punica granatum L.): A Review. Altern Med Rev 2008;13(2): 128-44.
  10. Li Y, Guo C,  Yang J, Wei J, Xu J, Shuang Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem 2006;  96 : 254–260.
  11. Duhoranimana E, Karangwa E, Lai Lingfeng, Xu, Xing, Yu, Jingyang, Xia, Shuqin, et al. Effect of sodium carboxymethyl cellulose on complex coacervates formation with gelatin: Coacervates characterization stabilization and formation mechanism. Food Hydrocolloids. 2017; 69: 111–120.
  12. Muhoza B, Xia S, Cai J, Zhang X, Duhoranimana E, Su J. Gelatin and pectin complex coacervates as carriers for cinnamaldehyde: Effect of pectin esterification degree on coacervate formation, and enhanced thermal stability. Food Hydrocolloids 2019; 87: 712–722.
  13. Toker OS, Konar N, Rasouli Pirouzian H, Oba S, Genc Polat D, Palabiyik I, Poyrazoglu, ES, Sagdic O. Developing functional white chocolate by incorporating different forms of EPA and DHA - Effects on product quality. LWT - Food Sci. Technol 2018; 87: 177-185.
  14. Todorovic V, Redovnikovic IR, Todorovic Z, Jankovic G, Dodevska M, Sobajic S. Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia. J Food Compos Anal. 2015; 41: 137-143.
  15. Singleton VL, Orthofer R, Lamuela-Ravent, RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 1999; 299: 152-78.
  16. Ruiz-Navajas Y, Viuda-Martos M, Sendra E, Perez-Alvarez J A, Fernández-López J. In vitro antibacterial and antioxidant properties of chitosan edible films incorporated with Thymus moroderi or Thymus piperella essential oils. J Food Control. 2013; 30, 386–392.
  17. Farzanmehr H, Abbasi S, Sahari MA. Effect of sugar replacer on some physicochemical, rheological and sensory properties of milk chocolate. Iran J Nutr Sci Food Technol 2008; 3(3):65–82 [in Persian].
  18. Glicerina V, Balestra F, Rosa M D, Romani S. Rheological, textural and calorimetric modifications of dark chocolate during process. J. Food Eng 2013; 119(1): 173-179.
  19. Botelho PB, Galasso M, Dias V, Mandrioli M, Lobato LP, Rodriguez-Estrada MT. Castro IA. Oxidative stability of functional phytosterol-enriched dark chocolate. LWT- Food Sci Technol 2014; 55(2): 444-451.
  20. Lindecrantz A. Investigation of seedpowder technology for pre-crystallization processing for dark chocolate- Effect on fat crystal structure and storage stability. 2014; Master of Science Thesis in the Master Degree Program Biotechnology. Gothenburg, Sweden: Chalmers University of Technology.
  21. Bolenz S, Holm M, Langkrär C. Improving particle size distribution and flow properties of milk chocolate produced by ball mill and blending. Eur. Food Res. Technol 2014; 238(1): 139–147.
  22. Pajin B, Dokić Lj, Zarić D, Šoronja-Simović D, Lončarević I, Nikolić I. Crystallization and rheological properties of soya milk chocolate produced in a ball mill. J. Food Eng2013; 114(1): 70–74.